Ring1b a302 869a

Manufactured by Fortis Life Sciences

Sourced in United Kingdom

RING1B (A302-869A) is a lab equipment product developed by Fortis Life Sciences. It serves as a tool for researchers and scientists to study the RING1B protein, which plays a role in the regulation of gene expression and chromatin remodeling.

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Lab products found in correlation

Ha ab9110, by Abcam (1 mentions) Sc 33613, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ring1b a302 869a

Generating Antibodies for SCML2 Protein

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Rabbit antibody against SCML2 was generated using an antigen consisting of a central region of SCML2 fused to GST and affinity-purified using the same antigen fused to a 6xHis tag. The following antibodies were used for IP and ChIP experiments: HA (ab9110; Abcam, Cambridge, England), BMI1 (A301-694A; Bethyl, Montgomery, TX), RING1B (A302-869A; Bethyl), GAL4 (06-262; Millipore). Antibodies against GST (sc-33613; Santa Cruz Biotechnology, Santa Cruz, CA) or RBP1 (Reinberg lab) were used for Western Blots.

Bonasio R., Lecona E., Narendra V., Voigt P., Parisi F., Kluger Y, & Reinberg D. (2014). Interactions with RNA direct the Polycomb group protein SCML2 to chromatin where it represses target genes. eLife, 3, e02637.

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Chromatin Profiling by CUT&RUN

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CUT&RUN experiments were performed as described (Skene & Henikoff, 2017) (link). The antibodies we used were from Cell Signaling Technology H3K27me3 (9733S), EZH2 (5246S), SUZ12 (3737S) and Bethyl laboratories RING1b (A302-869A). Libraries were constructed as described in the RNA-seq section. Two replicates were completed and representative experiments are shown.

Miller S.A., Damle M., & Kingston R.E. (2020). H3K27me3 is dispensable for early differentiation but required to maintain differentiated cell identity.

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