Ventana iview dab detection kit

Manufactured by Roche

Sourced in United States

The Ventana iView DAB Detection Kit is a laboratory reagent used in immunohistochemistry (IHC) procedures. The kit provides the necessary components to detect the presence of specific target proteins in tissue samples using a chromogenic detection method.

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Lab products found in correlation

Benchmark xt, by Roche (2 mentions) Amab90655, by Atlas Antibodies (1 mentions) Imagescope aperio analysis software, by Leica (1 mentions) Anti cleaved caspase 3 antibody, by Biocare Medical (1 mentions) Anti ki67, by Biocare Medical (1 mentions) Foxp3 antibody, by Abcam (1 mentions) Biotin sp conjugated affinipure goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Ventana benchmark ultra, by Roche (1 mentions)

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IVIEW DAB Detection Kit (139 citations) DAB detection kit (57 citations) IView DAB (4 citations) DAB kit (4 citations)

7 protocols using ventana iview dab detection kit

IHC Staining of PGK1 in ESCC

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To detect the expression of PGK1 in the tissue by IHC staining, 3-μm-thick sections from each FFPE tissue block were de-waxed with xylene and rehydrated through a graded series of ethanol, prepared by Zhongshan Hospital, Fudan University.
Total PGK1 immunostaining was performed on representative samples from normal to progressive ESCC. The IHC assay using PGK1 rabbit antibody (Wuhan Fine Biotech Co., Ltd, Catalog: FNab06354, dilution 1:200) was performed with Ventana iView DAB Detection Kit on a BenchMark XT automated staining system (Ventana Medical Systems, Tucson, AZ). Normal IgG from the same species of primary antibody diluted to match the concentration of the primary antibody was used as the negative control. For PGK1 negative cases, the experiments were repeated on the whole section in order to exclude heterogeneity. For assessment of staining, slides were scanned with the ScanScope System (Aperio, CA) and viewed with ImageScope (Aperio).

Li L., Jiang D., Zhang Q., Liu H., Xu F., Guo C., Qin Z., Wang H., Feng J., Liu Y., Chen W., Zhang X., Bai L., Tian S., Tan S., Xu C., Song Q., Liu Y., Zhong Y., Chen T., Zhou P., Zhao J.Y., Hou Y, & Ding C. (2023). Integrative proteogenomic characterization of early esophageal cancer. Nature Communications, 14, 1666.

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Quantitative RBM3 Immunohistochemistry Protocol

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Immunohistochemistry was performed by using a Ventana automated system and Ventana iVIEW DAB detection kit (Ventana Medical Systems, Inc, Tucson, Arizona) at the immunohistochemistry laboratory of McGill University Health Centre. Mouse antihuman monoclonal RBM3 antibody AMAb90655 (Atlas Antibodies, Stockholm, Sweden) was used. Nuclear staining intensity was evaluated semi-quantitatively by two independent evaluators (LF and FB) as follows: 0 = negative, 1 = weak staining, 2 = moderate staining, and 3 = intense staining. The few discrepancies that existed between the two evaluators were discussed, and a consensus was reached. For each core, the percentages of stained tumor cells of each intensity were estimated. The H-score, defined as the sum of the product of the percentage of tumor cells showing RBM3 labeling (0–100) multiplied by the labeling intensity (0–3), was calculated. For statistical purposes, the H-score results of sample duplicates were averaged in order to obtain a single value.

Florianova L., Xu B., Traboulsi S., Elmansi H., Tanguay S., Aprikian A., Kassouf W, & Brimo F. (2015). Evaluation of RNA-binding motif protein 3 expression in urothelial carcinoma of the bladder: an immunohistochemical study. World Journal of Surgical Oncology, 13, 317.

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Multiplex IHC Profiling of MMR and PD-L1

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IHC for four MMR proteins (MLH1, MSH2, MSH6, and PMS2) and PD-L1 was performed on TMAs. IHC analysis of the above-mentioned proteins used the following primary antibodies: mouse anti-human MLH-1 (clone ES05; Dako, Glostrup, Denmark), mouse anti-human MSH-2 (clone FE11; Dako, Glostrup, Denmark), rabbit anti-human MSH-6 (clone EP49; Dako, Glostrup, Denmark), rabbit anti-human PMS2 (clone EP51; Dako, Glostrup, Denmark), and rabbit anti-human PD-L1 (SP142; OriGene Technologies, Maryland, USA), and was performed with the Ventana iView DAB Detection Kit on a BenchMark XT automated staining system (Ventana Medical Systems, Tucson, AZ).

Jiang D., Song Q., Wei X., Yu Z., Liu Y., Wang H., Wang X., Huang J., Su J., Hong Y., Xu Y., Xu C, & Hou Y. (2022). PMS2 Expression With Combination of PD-L1 and TILs for Predicting Survival of Esophageal Squamous Cell Carcinoma. Frontiers in Oncology, 12, 897527.

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Immunohistochemistry Staining Protocol for ABCC2 and LGR5

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IHC was performed using a Ventana automated system and Ventana Iview DAB Detection Kit (Ventana Medical Systems, Inc., Tucson, AZ). IHC staining of ATP binding cassette subfamily C member 2 (ABCC2) and leucine-rich repeatcontaining G-protein coupled receptor 5 (LGR5) was performed by the streptavidin-biotin-peroxidase complex protocol using an ABCC2-specific mouse monoclonal antibody (Monosan, Uden, the Netherlands; catalog number MON9026; dilution 1:200) and an LGR5-specific mouse monoclonal antibody (dilution 1:100). A standard previously published protocol was used. 12 IHC Scoring, Quantitative Image Analysis, and Statistical Analysis Slides stained with BCL2, CK7, and LGR5 were scanned and analyzed with the Aperio ImageScope analysis software version 12.3.2.8013 (Leica Biosystems, Wetzlar, Germany). The algorithm used (nsr) combines staining intensity and percentage to provide a quantitative measurement of strong positive staining cells.
b-Cateninestained tissue slides, as well as the PRCC tissue microarray stained slides, were assessed manually using a combined intensity and percentage scoring via two independent pathologists (R.M.S. and G.M.Y.; each category has a score from 1 to 3, and then both scores are added together). 38, 39 End-stage (n = 3)

Saleeb R.M., Farag M., Ding Q., Downes M., Bjarnason G., Brimo F., Plant P., Rotondo F., Lichner Z., Finelli A, & Yousef G.M. (2019). Integrated Molecular Analysis of Papillary Renal Cell Carcinoma and Precursor Lesions Unfolds Evolutionary Process from Kidney Progenitor-Like Cells. The American journal of pathology, 189(10).

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Immunohistochemical Analysis of Apoptosis and Proliferation Markers

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The paraffin-embedded tissues were sectioned at 5 μm. The BenchMark XT automated slide staining system (Ventana Medical Systems, Inc., Tucson, Arizona) was used for the optimization and staining of all antibodies. The Ventana iView DAB detection kit (Roche Diagnostics, Indianapolis, IN, USA) was used as the chromogen and the slides were counterstained with hematoxylin. Omission of the primary antibody served as the negative control. Anti-cleaved caspase-3 antibody (BioCare Medical, Concord, CA, USA) was diluted 1:100 and incubated for 1 h and 32 min, and anti-Ki67 (BioCare Medical) was diluted 1:100 and incubated for 2 h. Foxp3+ antibody (Ab20034, Abcam, Waltham, Boston, MA, USA) was diluted 1:100. The secondary antibody used is a Biotin-SP-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) diluted 1:1000 from Jackson ImmunoResearch (West Grove, PA, USA).

Zhuang Y., Coppock J.D., Haugrud A.B., Lee J.H., Messerli S.M, & Miskimins W.K. (2024). Dichloroacetate and Quercetin Prevent Cell Proliferation, Induce Cell Death and Slow Tumor Growth in a Mouse Model of HPV-Positive Head and Neck Cancer. Cancers, 16(8), 1525.

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Immunohistochemical Staining of GFP

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Tissues were processed on a Leica 300 ASP tissue processor (Buffalo Grove IL) and sectioned at 5 μm. The BenchMark XT automated slide staining system (Medical Systems Inc., Tucson, AZ) was used for antibody labeling with chicken anti-GFP (1 : 250, ab13970, Abcam, Cambridge UK). Antigen retrieval was performed using the Ventana CC1 solution (950-124, Roche, Basel, Switzerland), a basic pH Tris-based buffer, and the Ventana iView DAB detection kit (760-091, Roche) was used as the chromogen. Slides were counterstained with hematoxylin and scanned using a Leica Aperio VERSA digital slide scanner.

Dunigan-Russell K., Silverberg M., Lin V.Y., Li R., Wall S.B., Li Q., Nicola T., Gotham J., Crowe D.R., Vitiello P.F., Agarwal A, & Tipple T.E. (2020). Club Cell Heme Oxygenase-1 Deletion: Effects in Hyperoxia-Exposed Adult Mice. Oxidative Medicine and Cellular Longevity, 2020, 2908271.

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Immunohistochemical Analysis of FCGBP and TFF3

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Formalin-fixed, paraffin-embedded serial tissue sections (3 µm) were dewaxed in xylol and rehydrated by descending concentrations of ethanol. For each specimen standard, hematoxylin and eosin (HE) staining, Alcian blue/PAS-staining and immunohistochemistry were performed. For antigen detection, we used the automated immunohistochemistry slide staining system VENTANA BenchMark ULTRA (Roche Diagnostics GmbH, Mannheim, Germany), the VENTANA iVIEW DAB Detection Kit (Roche Diagnostics GmbH) and the indirect biotin-streptavidin method before counterstaining with haemalaun solution. Antigen retrieval was performed with CC1mild (pH 8.5, 36 min, 95 °C) or CC2mild (pH 6.0, 44 min, 91 °C), respectively, followed by incubation with specific primary antibodies recognizing FCGBP (see Section 4.3) or TFF3 (see Section 4.3), at 36 °C for 32 min, dilution 1:500.

Laskou A., Znalesniak E.B., Harder S., Schlüter H., Jechorek D., Langer K., Strecker C., Matthes C., Tchaikovski S.N, & Hoffmann W. (2024). Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier. International Journal of Molecular Sciences, 25(4), 2287.

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