Sc 17768

Manufactured by Santa Cruz Biotechnology

SC-17768 is a lab equipment product from Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to provide a reliable and consistent tool for researchers in their scientific investigations. Further details on the specific features and intended use are not available.

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Lab products found in correlation

His tag, by Santa Cruz Biotechnology (1 mentions) Cfp c192, by American Type Culture Collection (1 mentions) Tsg101 c 2, by Santa Cruz Biotechnology (1 mentions) Ab2914, by Abcam (1 mentions) Sc 46692, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc 17768

Exosome Protein Profiling by Immunoblotting

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Exosomes (10 μg or 10⁹ particles) were resuspended in PBS with protease inhibitors. The suspension was mixed with Laemmli buffer, heated at 95 °C for 5 min, and chilled on ice for 5 min before loading onto SDS gel. Immunoblots probed with antibodies for proteins: Tsg-101 (C-2, 1:1000, Santa Cruz), CFP (C192, 1:1000, ATCC), 19kDa-lipoprotein (IT-19, 1:1000, ATCC), His-tag (1:500, Santa Cruz), CD81 (1:500, SBI), CD63 (1:500, Systems Bioscience), and, DsRed (632393, 1:1000, Clontech). Primary antibody incubation was followed with HRP-conjugated secondary antibodies (1:25,000, Pierce) and detected using enhanced chemiluminecence kit (Pierce). As loading controls, primary mouse mAbs against either alpha-Tubulin, 1/1000 dilution (Cat. T9026, Sigma) or Lamp-1 1/500 dilution (SC-17768, Santa Cruz Biotechnology or 1D4B, DHSB) were used.

Smith V.L., Cheng Y., Bryant B.R, & Schorey J.S. (2017). Exosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection. Scientific Reports, 7, 43578.

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Endocytic Trafficking of β-arrestin in N2A-D2R Cells

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Briefly, N2A-D2R cells were stimulated with vehicle or quinpirole (1μM, 5 or 30 min) followed by fixation, permeabilization and blocking with 6% horse serum in PBS. Cytoplasmic β-arrestin and Rab5 (a marker for the early endosome), transferrin receptors (a marker for the recycling endosome) or LAMP1 (a marker for the late endosome) were probed with the following primary antibodies: rabbit anti-β-arrestin (ab2914, Abcam), mouse anti-Rab5 (sc-46692, Santa Cruz), goat anti-Transferrin (ab166929, Abcam), and mouse anti-LAMP1 (sc-17768, Santa Cruz). Then cells were incubated with rabbit anti-mouse Alexa 405, mouse anti-rabbit Alexa 555 and donkey anti-goat Alexa 633, respectively. Colocalization of β-arrestin with Rab5, transferrin receptors or LAMP1 was calculated using Coloc2 software (FIJI). Data are presented relative to the level of colocalization between β-arrestin and each endocytic marker in control cells with vehicle treatment.

Luessen D.J., Hinshaw T.P., Sun H., Howlett A.C., Marrs G., McCool B.A, & Chen R. (2016). RGS2 modulates the activity and internalization of dopamine D2 receptors in neuroblastoma N2A cells. Neuropharmacology, 110(Pt A), 297-307.

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