Two types of high-resolution microscopes were used in this study. The LSM 880 inverted confocal laser scanning microscope with Airyscan (Carl Zeiss Microscopy) accompanied with 63×/1.40 numerical aperture (NA) plan-apochromat differential interference contrast (DIC) oil immersion objective and 32-channel gallium arsenide phosphide (GaAsP)-photomultiplier tubes (PMT) area detector and 488 nm, 561 nm and 633 laser lines was used in this study. Images were acquired and processed using ZEN imaging software (Zeiss). The Leica TCS SP8 gated STED super-resolution confocal microscope (Leica Microsystems) is coupled with Leica harmonic compound (HC) plan apochromatic (PL APO) 100×/1.40 oil STED objective and Leica gated HyD hybrid detector. Briefly, a white light excitation laser accompanied with 592 nm, 660 nm and 775 nm depletion lasers was used in this study. Images were acquired using LAS X software (Leica Microsystems) and final images were deconvolved using Huygens deconvolution software (Huygens Essentials, Scientific Volume Imaging).
Gated hyd hybrid detector
Manufactured by Leica
The Leica gated HyD hybrid detector is a high-performance detector designed for confocal microscopy applications. It combines the sensitivity of a photomultiplier tube (PMT) with the low-noise characteristics of a hybrid detector. The gated feature allows for the reduction of background signal and improved signal-to-noise ratio.
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Lab products found in correlation
Las x software, by Leica (2 mentions)
Lsm 880, by Zeiss (2 mentions)
Zen imaging software, by Zeiss (2 mentions)
Airyscan, by Zeiss (2 mentions)
Tcs sp8 gated sted super resolution confocal microscope, by Leica (2 mentions)
Harmonic compound hc plan apochromatic pl apo 100 1.40 oil sted objective, by Leica (2 mentions)
2 protocols using gated hyd hybrid detector
1
High-Resolution Microscopy Techniques
2
Multimodal Microscopy for Superresolution Imaging
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Two types of high-resolution microscopes were used in this study. The LSM 880 inverted confocal laser scanning microscope with Airyscan (Carl Zeiss Microscopy) accompanied with 63×/1.40 numerical aperture (NA) plan-apochromat differential interference contrast (DIC) oil immersion objective and 32-channel gallium arsenide phosphide (GaAsP)-photomultiplier tubes (PMT) area detector and 488 nm, 561 nm and 633 laser lines was used in this study. Images were acquired and processed using ZEN imaging software (Zeiss). The Leica TCS SP8 gated STED superresolution confocal microscope (Leica Microsystems) is coupled with Leica harmonic compound (HC) plan apochromatic (PL APO) 100×/1.40 oil STED objective and Leica gated HyD hybrid detector. Briefly, a white light excitation laser accompanied with 592 nm, 660 nm and 775 nm depletion lasers was used in this study. Images were acquired using LAS X software (Leica Microsystems) and final images were deconvolved using Huygens deconvolution software (Huygens Essentials, Scientific Volume Imaging).
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