The insulin secreting cell line MIN6 was cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (FBS, Gibco, Thermo Fisher), 50 IU/mL penicillin, 50 mg/mL streptomycin, and 70 mmol/L β-mercaptoethanol (Millipore). The cells were maintained at 37°C in a humidified atmosphere of 5% CO2. Recombinant mouse interleukin-1β (IL-1β) was purchased from Sigma. IFN-γ and TNF-α were purchased from R&D Systems. Hoechst dye 33342 was purchased from Beyotime (Jiangshu, China).
Recombinant mouse interleukin 1β il 1β
Manufactured by Merck Group
Recombinant mouse interleukin-1β (IL-1β) is a laboratory-produced version of a naturally occurring protein found in mice. IL-1β is a cytokine that plays a role in the immune response and inflammation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
β mercaptoethanol, by Merck Group (2 mentions)
Tnf α, by R&D Systems (2 mentions)
Ifn γ, by R&D Systems (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Hoechst 33342 dye, by Beyotime (1 mentions)
2 protocols using recombinant mouse interleukin 1β il 1β
1
Culturing MIN6 Beta Cells
2
Mouse MIN6 Cell Culture Protocol
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The mouse insulin secreting cell MIN6 was cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco Laboratories, Grand Island, NY, USA) supplemented with 15% heat-inactivated fetal calf serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA), 50 IU/mL penicillin and 50 mg/mL streptomycin (Thermo Fisher Scientific), and 70 mmol/L β-mercaptoethanol (Millipore), at 37°C in a humidified atmosphere of 5% CO2. Recombinant mouse interleukin-1β (IL-1β) was obtained from Sigma. IFN-γ and TNF-α were purchased from R&D Systems.
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