E coli dmt cells

High-fidelity DNA polymerase, restriction endonuclease (NdeI, XhoI), and dNTP were purchased from TaKaRa (Otsu, Japan). pEASY-E2 expression vector and pMD19-T vector were purchased from TaKaRa (Otsu, Japan). pPICZA expression vector was purchased from Invitrogen (Shanghai, China). The plasmid mini-prep kit and DNA gel extraction kit were purchased from Omega (Taipei, USA). One Step cloning kit was purchased from Vazyme biotech (Nanjing, China). Fast MultiSite Mutagenesis System and Bradford protein assay kit were purchased from TransGen (Beijing, China). Escherichia coli Trans1-T1 cells, E. coli BL21 (DE3) cells, and E. coli DMT cells were purchased from TransGen (Beijing, China). All other chemicals were of analytical grade and commercially available.

High fidelity DNA polymerase, restriction endonuclease (EcoRI, NotI) and dNTP from TaKaRa (Otsu, Japan). Plasmid mini-prep kit and DNA gel extraction kit from Omega (Taipei, USA). One-step cloning kit from Vazyme biotech (Nanjing, China). Fast MultiSite Mutagenesis System, Bradford protein assay kit, Escherichia coli Trans I-T1 cells and E.coli DMT cells from TransGen (Beijing, China). All other chemicals were of analytical grade and commercially available.