Co trimoxazole

DEC isolates were subjected to antimicrobial susceptibility testing. The disk diffusion test was performed according to Clinical and Laboratory Standards Institute guidelines for amikacin (30 μg/disk), gentamicin (10 μg/disk), ciprofloxacin (5 μg/disk), levofloxacin (5 μg/disk), tetracycline (30 μg/disk), chloramphenicol (30 μg/disk), co-trimoxazole (1.25/23.75 μg/disk), cefoperazone-sulbactam (75/30 μg/disk), ceftazidime (30 μg/disk), and ceftriaxone (30 μg/disk); all these disks were procured from Bio-Rad, USA. ATCC 25922 (E. coli) was used as a quality control for antimicrobial susceptibility testing [18 ].

Antimicrobial susceptibility testing was performed according to the 2016 recommendations of the Antibiogram Committee of the French Society for Microbiology¹. Disk diffusion method was performed for amikacin, amoxicillin +/- clavulanic acid, aztreonam, cefalotin, cefepime, cefoxitin, cefpirom, cefotaxime, ceftazidime, chloramphenicol, ciprofloxacin, colistin, cotrimoxazole, fosfomycin, gentamicin, imipenem, isepamicin, levofloxacin, moxalactam, nalidixic acid, netilmicin, ofloxacin, piperacillin, piperacillin/tazobactam, tetracycline, ticarcillin +/- clavulanic acid, and tobramycin (Bio-Rad^®). E-test (bioMérieux^®) was performed when inhibition zone diameters were observed around disks, and minimal inhibitory concentration (MIC) results that differed by more than two dilutions were considered as different. For ciprofloxacin, MICs were determined by the agar dilution reference method.