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Mcaspase 1

Manufactured by Adipogen
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MCaspase-1 is a recombinant mouse caspase-1 protein. Caspase-1 is a key enzyme involved in the processing and activation of inflammatory cytokines. This product can be used for in vitro research purposes.

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Lab products found in correlation

Citrullinated histone h3, by Abcam (2 mentions) Mnlrp6, by Merck Group (2 mentions) Hnlrp6, by Abcepta (2 mentions) Hnlrp3, by Cell Signaling Technology (2 mentions) Mil 1β, by Abcam (2 mentions) Complete protease phosphatase inhibitor, by Roche (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Triton x 100, by Roche (2 mentions)

2 protocols using mcaspase 1

1

Lung Cytokine Profiling by ELISA and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting for lung homogenates and cell lysates was performed as described in earlier publications22 , 30 (link). Antibodies to mCaspase-1 (Adipogen), mIl-1β (Abcam), mNLRP6 (Sigma), hNLRP6 (Abgent), hNLRP3 (Cell Signaling), citrullinated histone H3 (Abcam) and GAPDH (Cell signaling) were used for immunoblotting. Human IL-1β and IL-18 and mouse TNF-α, MCP-1, IL-1β, IL-6, G-CSF, and IL-17A (eBioscience), and mouse CXCL1, CXCL2, and CXCL5 (R&D systems) were quantified according to the manufacturers’ protocols. To perform ELISA in mouse lung homogenates, snap frozen lung lobes were thawed, homogenized in 2 mL PBS containing 0.1% triton X-100 supplemented with complete protease/phosphatase inhibitor (1 tablet/50 mL) (Roche), centrifuged, and filtered prior to use for ELISA. Readings were normalized to total protein of lung homogenates and expressed as pg/mg.
Cai S., Paudel S., Jin L., Ghimire L., Taylor C.M., Wakamatsu N., Bhattarai D, & Jeyaseelan S. (2020). NLRP6 Modulates Neutrophil Homeostasis in Bacterial Pneumonia-Derived Sepsis. Mucosal immunology, 14(3), 574-584.
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2

Lung Cytokine Profiling by ELISA and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting for lung homogenates and cell lysates was performed as described in earlier publications22 , 30 (link). Antibodies to mCaspase-1 (Adipogen), mIl-1β (Abcam), mNLRP6 (Sigma), hNLRP6 (Abgent), hNLRP3 (Cell Signaling), citrullinated histone H3 (Abcam) and GAPDH (Cell signaling) were used for immunoblotting. Human IL-1β and IL-18 and mouse TNF-α, MCP-1, IL-1β, IL-6, G-CSF, and IL-17A (eBioscience), and mouse CXCL1, CXCL2, and CXCL5 (R&D systems) were quantified according to the manufacturers’ protocols. To perform ELISA in mouse lung homogenates, snap frozen lung lobes were thawed, homogenized in 2 mL PBS containing 0.1% triton X-100 supplemented with complete protease/phosphatase inhibitor (1 tablet/50 mL) (Roche), centrifuged, and filtered prior to use for ELISA. Readings were normalized to total protein of lung homogenates and expressed as pg/mg.
Cai S., Paudel S., Jin L., Ghimire L., Taylor C.M., Wakamatsu N., Bhattarai D, & Jeyaseelan S. (2020). NLRP6 Modulates Neutrophil Homeostasis in Bacterial Pneumonia-Derived Sepsis. Mucosal immunology, 14(3), 574-584.
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