Human GBC cell lines NOZ, GBC-SD, SGC-996, EH-GB-1, and EH-GB-2 were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, People’s Republic of China). NOZ cells were maintained in William’s medium (Gibco, Grand Island, NY, USA) supplemented with 100 U/mL penicillin–streptomycin (Hyclone, Logan, UT, USA) and 10% fetal bovine serum (FBS; Gibco). GBC-SD cells were maintained in DMEM (Dulbeccos’ Modified Eagle’s Medium) medium (Gibco) containing 10% FBS. These cell lines were cultured at 37°C in a humidified incubator containing 5% CO2.
Eh gb 1
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The EH-GB-1 is a cell culture incubator designed for maintaining optimal growth conditions for a variety of cell lines. It features precise temperature and humidity control, as well as a CO2 regulation system to ensure a stable, well-regulated environment for cell cultivation.
Automatically generated - may contain errors
Lab products found in correlation
Gbc sd, by Thermo Fisher Scientific (4 mentions)
Gbc sd, by National Collection of Authenticated Cell Cultures (4 mentions)
Sgc 996, by National Collection of Authenticated Cell Cultures (4 mentions)
William s medium, by Thermo Fisher Scientific (3 mentions)
Sgc 996, by Thermo Fisher Scientific (3 mentions)
Eh gb1, by Thermo Fisher Scientific (3 mentions)
Eh gb 2, by National Collection of Authenticated Cell Cultures (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Paxillin, by Cell Signaling Technology (1 mentions)
Vinculin, by Cell Signaling Technology (1 mentions)
Ehgb 2, by Thermo Fisher Scientific (1 mentions)
Ocug 1, by Thermo Fisher Scientific (1 mentions)
Ocug 1 cell line, by Health Science Research Resources Bank (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Recombinant human tgf β1, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Puromycin, by MedChemExpress (1 mentions)
4 protocols using eh gb 1
1
Establishment and Maintenance of Human GBC Cell Lines
2
Establishing Human Gallbladder Cancer Cell Lines
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The human GBC cell lines NOZ, EH-GB-1, EH-GB-2, SGC-996 and GBC-SD were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The OCUG-1 cell line was obtained from the Health Science Research Resources Bank (Osaka, Japan). The NOZ cell line was maintained in William’s medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco). The GBC-SD cells were maintained in DMEM medium (Gibco). The EH-GB-1, EH-GB-2, SGC-996 and OCUG-1 cells were cultured in RPMI 1640 (Gibco). DHA was purchased from Selleck Chemicals and dissolved in DMSO. Primary antibodies against TCTP, vinculin, paxillin and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA).
3
Culturing Human Gallbladder Cancer Cell Lines
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The human gallbladder cancer cell lines GBC-SD, SGC-996, NOZ, OCUG, and EHGB-1 were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). GBC-SD, OCUG, and EHGB-1 cells were cultured in high-glucose DMEM (Gibco, USA). SGC996 cells were cultured in RPMI 1640 medium (Gibco). NOZ cells were cultured in William's medium (Gibco). All of the above media were supplemented with 10% fetal bovine serum (FBS, Gibco), 100 μg/mL streptomycin, and 100 U/mL penicillin (Hyclone, USA). The cells were maintained at 37°C in a humidified atmosphere with 5% CO2. For TGF-β treatment, cells were stimulated with 10 ng/mL human recombinant TGF-β1 (Peprotech, USA) diluted with serum-free medium containing BSA for 24 or 48 h.
4
Cell Culture Conditions for Gallbladder Cancer
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NOZ cells were obtained from the Health Science Research Resources Bank (Osaka, Japan), GBC-SD, SGC-996, and EH-GB1 cells were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences, and human embryonic kidney 293 T (HEK293T) cells were purchased from the American Type Culture Collection. NOZ and GBC-SD both were P53 WT genotype (P53+/+). GBC-SD, SGC-996, EH-GB1, and HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco), and NOZ cells were cultured in William’s E medium (Gibco). All cell lines were supplemented with 10% fetal bovine serum (Gibco), penicillin (100 mg ml−1) and streptomycin (100 mg ml−1) and were incubated in a humidified chamber with 5% CO2 at 37 °C. All cell cultures were ensured to be mycoplasma-negative cultures by monthly mycoplasma tests and were passaged with 0.25% trypsin containing 2.21 mm ethylenediaminetetraacetic acid (EDTA) in PBS when the cells reached 80~90% confluency. Gemcitabine (GEMZAR) was purchased from Eli Lilly. Cisplatin, cycloheximide, doxorubicin, and puromycin were purchased from MedChem Express.
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