Mouse anti vimentin antibody

Cells were transfected with miRNA oligonucleotides or inhibitors, as described in the section of oligonucleotide transfection, plated onto glass coverslips in Millicell EZ SLIDES (Merck Millipore, Bedford, MA, USA) and stained after 96 h. For E-Cadherin and Vimentin staining, cells were fixed in 4% paraformaldehyde, permeabilized in 0.1% Triton X-100 and probed with rabbit-anti-E-Cadherin antibody (1:200; Proteintech Technology, Chicago, IL, USA) or mouse-anti-Vimentin antibody (1:1000; Proteintech Technology) overnight at 4 °C. The primary antibody was detected using anti-rabbit-Alexa594 or anti-mouse-Alexa488-conjugated antibodies (1:200; Invitrogen). To detect nuclei, cells were co-stained with 4,6-diamidino-2-phenylindole. Fluorescence was observed and imaged using a ZEISS ScopeA1 microscope (Carl Zeiss, Jena, Germany).