Enzymatic colorimetric kit

Manufactured by Pars Azmoon

The Enzymatic Colorimetric Kit is a laboratory equipment used for the quantitative determination of various analytes in biological samples. It utilizes enzymatic reactions coupled with color-producing substrates to enable colorimetric detection and measurement of the target analyte.

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Lab products found in correlation

Specord 50 plus, by Analytik Jena (1 mentions)

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Enzymatic kits (23 citations)

5 protocols using enzymatic colorimetric kit

Fasting Blood Markers Evaluation

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At baseline and end of the intervention, after 10 to 12 hours of fasting 5 ml of
blood sample was taken from all participants. Serum samples were disjoined from
the whole blood by centrifugation at 2606.8×g for 10 minutes
and were stored at —80°C before analysis. The fasting venous blood samples were
assembled in ethylenediaminetetraacetic acid-treated vacutainers to evaluate
fasting blood glucose and inflammatory indices.
Colorimetric enzymatic kit (Pars Azmoon Co.) was used for measuring fasting blood
glucose concentration. TNF-α was also measured using ELISA method by Orgenium
kit. In addition, hs-CRP concentration was determined by means of ELISA (via
Hitachi911 chemistry and immunoassay(Sentinel) analysis).

Shareghfarid E., Nadjarzadeh A., Heidarzadeh-Esfahani N., Azamian Y, & Hajiahmadi S. (2022). The Effect of Flaxseed Oil Supplementation on Body Composition and Inflammation Indices in Overweight Adults With Pre-Diabetes. Nutrition and Metabolic Insights, 15, 11786388221090083.

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Hepatic Lipid Extraction and TG Quantification

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A small piece of frozen liver (~150 mg) was homogenized in 3 ml of chloroform/methanol (2:1). The hepatic lipids were extracted, using the method suggested by [14] . TG content was measured by colorimetric-enzymatic kit (Parsazmun, Iran) and expressed as mg/g tissue.

Afshari A., Salimi F., Babaie M., Bakhtiyari S., Hassanzadeh G., Shabani M., & Nowrouzi A. (2020). Differential Expression of Gluconeogenic Enzymes in Early- and Late- Stage Diabetes: The Effect of Citrullus Colocynthis Seed Extract on Hyperglycemia and Hyperlipidemia.

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Serum Creatine Phosphokinase Assay

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The activity of serum creatine phosphokinase (CPK) was assayed by an enzymatic colorimetric kit (Pars Azmoon Co., Tehran, Iran). Based on the kit's procedure, creatine kinase converts creatine into ADP and phosphocreatine. The absorbance change per minute was detected at 340 nm, and its data were expressed as U/L.

Gholami A., Ataei S., Ahmadimoghaddam D., Omidifar N, & Nili-Ahmadabadi A. (2021). Pentoxifylline Attenuates Arsenic Trioxide-Induced Cardiac Oxidative Damage in Mice. Oxidative Medicine and Cellular Longevity, 2021, 6406318.

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Serum Lactate Dehydrogenase Activity Assay

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Lactate dehydrogenase (LDH) activity in serum sample was measured by determining the rate of oxidation of NADH by an enzymatic colorimetric kit (Pars Azmoon Co., Tehran, Iran). The absorbance change per minute was detected at 340 nm using spectrophotometric instrument (Analytik Jena Specord 50 Plus), and its results were expressed as U/L.

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Hippocampal LDH Activity Assay

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Lactate dehydrogenase (LDH) activity in the hippocampus homogenate sample was determined by measuring the rate of oxidation of NADH using an enzymatic colorimetric kit (Pars Azmoon Co., Tehran, Iran). The change of absorbance was detected at 340 nm and results were expressed as unit/mg protein.

Nili-Ahmadabadi A., Ali-Heidar F., Ranjbar A., Mousavi L., Ahmadimoghaddam D., Larki-Harchegani A, & Ghafouri-Khosrowshahi A. (2018). Protective effect of amlodipine on diazinon-induced changes on oxidative/antioxidant balance in rat hippocampus. Research in Pharmaceutical Sciences, 13(4), 368-376.

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