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Anti cd8 pacific blue

Manufactured by Bio-Rad
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The Anti-CD8-Pacific Blue is a fluorescent-labeled antibody that specifically binds to the CD8 surface marker. It is a tool used in flow cytometry applications to identify and analyze CD8-positive cells.

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Lab products found in correlation

Golgiplug, by BD (2 mentions) Cytofix cytoperm kit, by BD (2 mentions) Ca2 ionomycin, by Merck Group (2 mentions) Brefeldin a, by Bio-Rad (2 mentions) Anti cd4 fitc, by Bio-Rad (2 mentions) Anti cd3, by Bio-Rad (1 mentions)

2 protocols using anti cd8 pacific blue

1

Quantifying IFN-γ Production in T Cells

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For assaying IFNγ production, 4 × 105 PBMCs were stimulated for 5 h in the presence of 2 ng/mL PMA, 4 μg/mL Ca2+ ionomycin (Sigma-Aldrich), and Brefeldin A (BD GolgiPlug; BD Biosciences, San Jose, CA, USA) adapted from described stimulation conditions [32 (link)] or overnight with 40 μg/mL T. cruzi lysate at 37 °C. Brefeldin A was added 5 h prior to end of incubation, and the cells were stained with anti-CD8-Pacific Blue and anti-CD4-FITC (AbD Serotec, Raleigh, NC, USA) followed by intracellular staining with anti-bovine IFN-γ AF647 (AbD Serotec) according to the BD Cytofix/Cytoperm kit (BD Biosciences). Samples were fixed in 2% formaldehyde prior to analysis by flow cytometry.
Hartley A.N., Cooley G., Gwyn S., Orozco M.M, & Tarleton R.L. (2014). Frequency of IFNγ-producing T cells correlates with seroreactivity and activated T cells during canine Trypanosoma cruzi infection. Veterinary Research, 45(1), 6.
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2

Canine Vaccine IFNγ Response Assay

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For assaying IFNγ levels, 4×105 PBMCs were stimulated for 5hr in the presence of 2ng/mL phorbol 12-myristate 13-acetate (PMA), 4µg/mL Ca2+ ionomycin (Sigma-Aldrich), and brefeldin A (BD GolgiPlug; BD Biosciences) (Pedersen et al., 2002 ). For polyclonal activation, PBMCs were plated with 15µg/mL anti-CD3 (AbD Serotec) or diluted whole vaccine antigens, incubated overnight at 37°C and brefeldin A added 5hr prior to end of incubation. Canine vaccines were IMRAB 3TF (Rabies; Merial, Athens, GA, USA), Duramune 5 (Canine distemper-Adenovirus Type 2-Parainfluenza-Parvovirus (DAPP); Fort Dodge Animal Health, Fort Dodge, IA, USA), and Leptovax 4 (Leptospirosis bacterial extract; Fort Dodge Animal Health) vaccines. Cells were stained with anti-CD8-Pacific Blue and anti-CD4-FITC (AbD Serotec) followed by intracellular staining with anti-bovine anti-IFN-γ AF647 (AbD Serotec) according to the BD Cytofix/Cytoperm kit (BD Biosciences).
Hartley A.N, & Tarleton R.L. (2015). CHEMOKINE RECEPTOR 7 (CCR7)-EXPRESSION AND IFNγ PRODUCTION DEFINE VACCINE-SPECIFIC CANINE T CELL SUBSETS. Veterinary immunology and immunopathology, 164(0), 127-136.
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