The embryo (25 d) and extra-embryonic membranes (25 and 45 d) were retrieved by uterine lavage [29 (link)]. The tissue samples were fixed in 10% formaldehyde for 24 h, transferred to 70% methanol and paraffin embedded [29 (link)]. The expression of eca-miR-409-3p was investigated by chromogenic ISH using a dual digoxigenin (DIG)-labeled LNA™ probe specific to hsa-miR-409-3p (610701-360; miRCURY LNA™, Exiqon, Vedbaek, Denmark) as previously described [29 (link)] on 25 d embryos (n = 3) along with the extra-embryonic membranes. A dual DIG-labeled LNA™ probe specific to U6 snRNA (#699002-360; Exiqon) and a scrambled miRNA probe (#699003-360; Exiqon) were used as positive and negative controls, respectively.
Scrambled mirna probe
Manufactured by Qiagen
Sourced in Denmark
The Scrambled miRNA probe is a laboratory equipment designed for the detection and analysis of microRNA (miRNA) expression. It serves as a negative control in miRNA studies, allowing researchers to evaluate the specificity of miRNA detection assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using scrambled mirna probe
1
Chromogenic ISH analysis of miR-409-3p
2
In Situ Hybridization of KSHV/EBV miRNAs in CLL
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miRNA-ISH for two KSHV/HHV8 miRNAs (KSHV-miR-K12-4-3p, KSHV-miR-K12-10b) and one EBV miRNA (EBV-miR-BHRF1-1) were performed in 8 BM biopsy specimens of patients with CLL as previously described (Ferrajoli et al., 2015 (link)). Briefly, double digoxigenin-labeled locked nucleic acid probes (LNA; Exiqon, Vedbaek, Denmark) antisense to the above miRNAs (Fig. 5 and Supplementary Figs. S4 and S8) were hybridized on tissue sections for 3 h at 55 °C. Detection was accomplished with anti-DIG alkaline phosphate Fab fragment followed by nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) color development (Ventana, Roche, Basel, CH). U6 probe reactivity was used as positive control, and the scrambled-miRNA probe (Exiqon Vedbaek, Denmark) was used as negative control for staining.
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