Blood samples were collected at euthanasia. Serum active GLP-1 concentration was measured using an insulin enzyme-linked immunosorbent assay (ELISA) kit (IBL, Osaka, Japan). In addition, the serum stromal cell-derived factor-1α (SDF-1α) and platelet-derived growth factor (PDGF) concentration was measured using ELISA kits DSA00 and MBB00, respectively (R&D Systems, Minneapolis, MN, USA). Urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) concentration was measured using a kit (JaICA, Shizuoka, Japan). Urinary creatinine levels were also measured using a kit (Wako, Osaka, Japan) to adjust the urine concentration, as previously described [23 (link)].
Mbb00
Manufactured by R&D Systems
Sourced in United States
MBB00 is a laboratory instrument designed for the measurement and analysis of various biological samples. The core function of this product is to provide reliable and accurate data on the characteristics of the samples under investigation. No further details on the intended use or specific applications of this product are provided.
Automatically generated - may contain errors
Lab products found in correlation
Mmv00, by R&D Systems (2 mentions)
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Db100b, by R&D Systems (1 mentions)
Mb100b, by R&D Systems (1 mentions)
Dbb00, by R&D Systems (1 mentions)
Mang20, by R&D Systems (1 mentions)
C3228, by Merck Group (1 mentions)
Dfb50, by R&D Systems (1 mentions)
P8340, by Merck Group (1 mentions)
4 protocols using mbb00
1
Biomarker Measurement in Euthanized Subjects
2
Quantification of TGF-β1 and PDGF-BB
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We determined the concentration of active TGF-β1 in the serum using the TGF-β1 ELISA Development kit (human: R&D Systems, DB100B; mouse: R&D Systems, MB100B) and PDGF-BB using PDGF-BB ELISA Development kit (human: R&D Systems, DBB00; mouse: R&D Systems, MBB00) following the manufacturer’s instructions.
3
Quantitative Tissue Biomarker Assay
4
Quantifying Growth Factors in Tumor Cells
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Enzyme-linked immunosorbent assay was used for measuring human FGF-2, mouse VEGF, and mouse PDGFB protein concentrations. To detect protein levels in tissues and cells, tumor tissues and cells in culture were homogenized in a lysis buffer (C3228, Sigma), containing proteinase inhibitor cocktails (1:100, P8340, Sigma), followed by 15 min centrifugation. The supernatants were transferred to a new collection tube and either immediately used or stored at −20 °C or −80 °C until further analysis. Conditioned media were collected from confluent E0771 and T241 cells at 24 and 48 h. Cell debris was removed by centrifugation and supernatants were stored at −20 °C or −80 °C until analysis. The human FGF-2 (DFB50, R&D systems), mouse VEGF (MMV00), and mouse PDGF-BB ELISA assays (MBB00, R&D Systems) were performed according to the manufacturer’s protocol with the standard curve. Absorbance values were detected at 450 nm using a microplate reader and values were calculated using the formula obtained from the trendline.
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