Uplc ms instrument

Melting points were determined by a Mettler Toledo FP 62 melting point apparatus and are uncorrected. ¹H NMR spectra were recorded at 400 MHz on a Varian Unitynova 400 NMR spectrometer using tetramethylsilane as an internal standard, and then ¹³C NMR spectra were recorded at 100 MHz. Mass spectra were run on a Waters UPLC-MS instrument. UV spectra were determined by a Lambda 25 spectrometer. HPLC spectra were run through a Waters SunfireTM C18, 5 µm, 4.6 mm × 250 mm column on a Waters apparatus (pump: e 2695; detector: 2998). TLC plates (GF 254) were bought from Branch Qingdao Haiyang Chemical Plant. All the solvents and commercial materials are bought from Sinopharm.

¹H NMR spectra were recorded at 400 MHz on a Varian Unity INOVA 400 MHz NMR spectrometer using tetramethylsilane as an internal standard, and ¹³C NMR spectra were recorded at 100 MHz. Mass spectra were run on a Waters UPLC-MS instrument. Melting points were determined by a Mettler Toledo FP62 melting point apparatus and are uncorrected. Thin-layer chromatography plates (GF 254) were bought from Branch Qingdao Haiyang Chemical Plant.