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Pe conjugated anti mouse siinfekl peptide bound to h 2kb

Manufactured by Thermo Fisher Scientific

The PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb is a laboratory reagent used for flow cytometry applications. It is a fluorescently labeled antibody that specifically binds to the SIINFEKL peptide when presented on the H-2Kb major histocompatibility complex on mouse cells. This product can be used to detect and quantify the presence of SIINFEKL-specific CD8+ T cells in mouse samples.

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2 protocols using pe conjugated anti mouse siinfekl peptide bound to h 2kb

1

Quantifying SIINFEKL-Presenting DCs in dLN

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To analyze OVA257–264 (SIINFEKL) peptide (Anaspec) presenting DCs, dLNs were isolated and digested on day 7 post vaccination. dLN cells were enumerated and stained with APC conjugated anti-mouse CD11c (eBioscience 17–0114) and PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb (eBioscience 12–5743) for 15 minutes on ice. Cells were washed and assessed by flow cytometry.
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2

Quantifying SIINFEKL-Presenting DCs in dLN

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze OVA257–264 (SIINFEKL) peptide (Anaspec) presenting DCs, dLNs were isolated and digested on day 7 post vaccination. dLN cells were enumerated and stained with APC conjugated anti-mouse CD11c (eBioscience 17–0114) and PE conjugated anti-mouse SIINFEKL peptide bound to H-2Kb (eBioscience 12–5743) for 15 minutes on ice. Cells were washed and assessed by flow cytometry.
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