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Alexa 594 goat anti mouse igg h l

Manufactured by Thermo Fisher Scientific
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Alexa 594 goat anti-mouse IgG (H+L) is a secondary antibody conjugated with the Alexa Fluor 594 dye. It is designed to detect and visualize mouse immunoglobulins (IgG) in various immunoassay applications.

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Lab products found in correlation

Ab13970, by Abcam (1 mentions) Click it edu alexa fluor 647 imaging kit, by Thermo Fisher Scientific (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg h l alexa488, by Thermo Fisher Scientific (1 mentions) Mms 435p, by Fortrea (1 mentions)

Close spelling variants of this product

Alexa Fluor® 594 goat anti-mouse IgG (H+L) secondary antibody Alexa Fluor 594 goat anti-mouse IgG (H+L) Alexa Fluor 594–goat anti-mouse IgG (H + L) cross-adsorbed secondary antibodies Alexa 594-conjugated goat anti-mouse IgG (H+L) Alexa Fluor™ 594 Goat Anti-Mouse IgG (H+L) antibody Alexa Fluor 594® F(ab’)2 fragment of goat anti-mouse IgG (H + L) F (ab’) 2-goat anti-mouse IgG (H+L) Alexa Fluor 594 (GAR48810900223, Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor™ 594 Goat anti-mouse IgG (H+L) Goat anti-mouse Alexa 594

4 protocols using alexa 594 goat anti mouse igg h l

1

Immunofluorescence Staining of Myofibers

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Myofibers were fixed in 4% paraformaldehyde for 10 min, treated with 0.5% triton and blocked in 10% goat serum/10% swine serum (Moyle and Zammit, 2014 (link)). The following antibodies were used: EPHB1 (Rabbit Abcam® ab5414) 1/100, PAX7 monoclonal (DSHB®, PAX7-c) 1/50, MYOD monoclonal (DAKO®, clone 5.8A, M3512) 1/60, MYOD (Rabbit Santa Cruz Biotech®, sc-760) 1/50, MYOG monoclonal (DSHB®, F5D) 1/50, CD-31 (PECAM-1) (Rat BD Pharmigen®, 550274) 1/500, and GFP (Rabbit Life Technologies®) 1/500, or (Chicken Abcam® ab13970) 1/200. Secondary antibodies employed to reveal the staining were Alexa 594 goat anti-mouse IgG (H+L), Alexa 488 goat anti-rabbit IgG (H+L) (Life Technologies®), and DyLight-405 donkey anti-chicken IgY (IgG) (H+L), and Cy5-Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch®). Nuclei were counterstained with DAPI.
Alonso-Martin S., Rochat A., Mademtzoglou D., Morais J., de Reyniès A., Auradé F., Chang T.H., Zammit P.S, & Relaix F. (2016). Gene Expression Profiling of Muscle Stem Cells Identifies Novel Regulators of Postnatal Myogenesis. Frontiers in Cell and Developmental Biology, 4, 58.
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2

Immunofluorescence Staining of Muscle Markers

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The following antibodies were used: MYOD, MYOG, and GFP (as above), PH3 (Rabbit Merck-Millipore®, 06-570) 1/50, Ki67 (BD Pharmigen®, 556003) 1/150, HA (Rabbit Sigma-Aldrich®, H6908) 1/400, and GFP monoclonal (Sigma®) 1/50. Secondary antibodies included Alexa 488 goat anti-mouse IgG (H+L), Alexa 594 goat anti-mouse IgG (H+L), Alexa 488 goat anti-rabbit IgG (H+L), Alexa 594 goat anti-rabbit IgG (H+L) (Life Technologies®). EdU reaction was performed with Click-iT® EdU Alexa Fluor® 647 Imaging Kit (ThermoFisher Scientific®). Nuclei were counterstained with DAPI.
Alonso-Martin S., Rochat A., Mademtzoglou D., Morais J., de Reyniès A., Auradé F., Chang T.H., Zammit P.S, & Relaix F. (2016). Gene Expression Profiling of Muscle Stem Cells Identifies Novel Regulators of Postnatal Myogenesis. Frontiers in Cell and Developmental Biology, 4, 58.
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3

Immunofluorescence Assay of HCV Infection

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Huh7.5 cells were infected with viruses H77/JFH1 or H77/JFH1L665W with a multiplicity of infection (MOI) equal to 0.01 and incubated for 24 hours. We plated 2.5x104 infected Huh7.5 cells per well in an 8 wells slide and incubated them for an additional 24 hours. Cells were fixed with para-formaldehyde at room temperature for 15 minutes and the cells were permeabilized with 0.1% Tween20. Antibody anti-NS5A in combination with either AR4A or AR5A was used as primary antibodies. We used a combination of Alexa594 goat anti-mouse IgG (H+L) (Invitrogen) and Alexa488 goat anti-human (Invitrogen) as secondary antibodies with a Hoechst 33342 (Molecular Probes) counterstain for nuclei.
Velázquez-Moctezuma R., Law M., Bukh J, & Prentoe J. (2017). Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A. PLoS Pathogens, 13(2), e1006214.
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4

Antibody Panel for Neural Cell Characterization

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Primary antibodies used for immunostaining and immunoblot included rabbit anti-MAP2 (CST, #4542, 1:1000), mouse anti-tubulin β III (Covance, MMS-435P, 1:5000), rabbit anti-tubulin β III (Covance, PRB-435P-100, 1:1000), chicken anti-beta-tubulin 3 (Aves Labs, AB_2313564, 1:1000), rabbit anti-p62/SQSTM1 (Abcam, ab109012, 1:1000), mouse anti-p62/SQSTM1 (CST, #88588, 1:1000), rabbit anti-STAT3 (CST, #4904, 1:1000), rabbit anti-GAPDH (Santa Cruz, sc-32233, 1:5000), rabbit anti-DARPP-32(19A3) (CST, #2306, 1:200), rat anti-CTIP2 (Abcam, ab18465, 1:500), rabbit anti-DLX1 (Millipore, AB5724, 1:100), rabbit anti-DLX2 (Abcam, ab135620, 1:100), and rabbit anti-MYT1L (Proteintech, 25234-1-AP, 1:500) antibodies. The secondary antibodies for immunostaining included Alexa-488 Goat anti-mouse IgG (H+L) (Invitrogen, A-11029, 1:2000), Alexa-488 Goat anti-rabbit IgG (H+L) (Invitrogen, A-11034, 1:2000), Alexa-568 Goat anti-mouse IgG (H+L) (Invitrogen, A-11031, 1:2000), Alexa-568 Goat anti-rabbit IgG (H+L) (Invitrogen, A-11016, 1:2000), Alexa-594 Goat anti-mouse IgG (H+L) (Invitrogen, A-11032, 1:2000), Alexa-594 Goat anti-rabbit IgG (H+L) (Invitrogen, A-11012, 1:2000), Alexa-647 Goat anti-chicken IgY (Invitrogen, A-21449, 1:2000), and Alexa-594 Goat anti-rat IgG (H+L) (Invitrogen, A-11007, 1:2000).
Oh Y.M., Lee S.W., Kim W.K., Chen S., Church V.A., Cates K., Li T., Zhang B., Dolle R.E., Dahiya S., Pak S.C., Silverman G.A., Perlmutter D.H, & Yoo A.S. (2022). Age-related Huntington’s disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy. Nature neuroscience, 25(11), 1420-1433.
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