Goat anti albumin

ApoB-100, apoAI, albumin, α-tubulin, and GFP-tagged mouse LDLR and human LDLR expression was determined by western blotting. For separation, 7% SDS-polyacrylamide gels were used for apoB-100, albumin, α-tubulin, and LDLR, whereas 10% SDS-polyacrylamide gels were used for apoAI. Molecular weights were determined by reference to a prestained protein ladder (Gene DireX, Taipei, Taiwan). Primary antibodies used for experiments were rabbit anti-apoB-100 (Acris Antibodies GmbH, Herford, Germany; diluted 500-fold), rabbit anti-apoAI (Santa Cruz Biotechnology, Inc., CA; diluted 200-fold), goat anti-albumin (Santa Cruz Biotechnology, Inc.; diluted 500-fold), rabbit anti-α-tubulin (Abcam, Cambridge, MA; diluted 1,000-fold) and rabbit anti-GFP (Life Technologies, Gaithersburg, MD; diluted 2,000-fold), and rabbit anti-LDLR (Novus, Littleton, CO; diluted 100-fold). For detection, membranes were incubated with horseradish peroxidase-labeled secondary antibody (anti-rabbit IgG (GE Healthcare) or anti-goat IgG (Santa Cruz Biotechnology, Inc.)) diluted 5,000-fold in TBS-T (TBS-Tween20) containing 0.1% bovine serum albumin (BSA) for 1 h at room temperature. Proteins were visualized using an ECL Prime Western Blotting Detection System (GE Healthcare) with a luminescent image analyzer (Bio-Rad Laboratories, Tokyo, Japan). Full length figures of membranes are shown in Supplementary Figs S5 and S8.