GeneChip Zebrafish Genome Microarrays (Affymetrix) were processed by the MCG Genomic Core. Each run involved sibling zebrafish and consisted of 23 RNA samples across each regenerative paradigm—1 pretreatment, and 11 time points from the control and Mtz treatment groups. RNA sample quality was assessed by concentration and the RNA Integrity Number (RIN), an algorithm that assigns integrity values to RNA measurements [106 (link)]. Only samples attaining a minimal concentration of ≥100ng/μL (5μL total) and a RNA integrity number (RIN) value of ≥8 were used for microarray hybridizations [106 (link)]. Runs where a minimum of 21 of 23 samples were deemed of good quality were utilized in the microarray studies. Three runs were processed per regenerative paradigm resulting in six microarray sets (S1 Table ) and a total 138 microarrays overall.
Genechip zebrafish genome microarrays
Manufactured by Thermo Fisher Scientific
The GeneChip Zebrafish Genome Microarrays are high-density oligonucleotide microarrays designed for the analysis of zebrafish gene expression. The arrays contain probes covering a comprehensive set of zebrafish transcripts, enabling researchers to study genome-wide expression patterns in this important model organism.
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Lab products found in correlation
2 protocols using genechip zebrafish genome microarrays
1
Zebrafish Transcriptomic Analysis via Affymetrix Microarrays
2
Zebrafish Genome Microarray Analysis
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GeneChip Zebrafish Genome Microarrays (Affymetrix) were processed by the MCG Genomic Core. Each run involved sibling zebrafish and consisted of 23 RNA samples across each regenerative paradigm-1 pretreatment, and 11 time points from the control and MTZ treatment groups. RNA sample quality was assessed by concentration and the RNA Integrity Number (RIN), an algorithm that assigns integrity values to RNA measurements (93) . Only samples attaining a minimal concentration of ≥100ng/µL (5µL total) and a RNA integrity number (RIN) value of ≥8 were used for microarray hybridizations (93) . Runs where a minimum of 21 of 23 samples were deemed of good quality were utilized in the microarray studies. Three runs were processed per regenerative paradigm resulting in six microarray sets (Supp. Table 1) and a total 138 microarrays overall.
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