Germ-free (GF) C57BL/6 wild-type (WT) mice were kept in germ-free flexible film isolators (Class Biologically Clean Ltd) at Rockefeller University. For wounding experiments, GF C57BL/6 mice were exported to isocages bioexclusion system (Tecniplast, PA, USA) and housed in isocages for the duration of the experiment. Wounding of GF mice was performed in a sterile hood using sterile autoclaved instruments. Wounding of specific-pathogen-free (SPF) C57BL/6 WT mice was performed in the same hood after GF mice were transferred into the isocages. Both GF and SPF mice were then housed in the isocages under the same conditions for 1 or 5 days as described before harvesting skin wounds. Mice housed in the isocages were provided with autoclaved food and water.
Isocages bioexclusion system
Manufactured by Tecniplast
Sourced in United States
The Isocages bioexclusion system is a laboratory equipment designed for housing rodents in a highly controlled environment. It features a sealed, filtrated cage system that isolates the animals from external contaminants, maintaining a stable and pathogen-free environment. The core function of the Isocages is to provide a secure and sterile enclosure for research animals, ensuring their health and the integrity of scientific experiments.
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Lab products found in correlation
2 protocols using isocages bioexclusion system
1
Wound Healing in Germ-Free Mice
2
Wound Healing in Germ-Free Mice
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Germ-free (GF) C57BL/6 wild-type (WT) mice were kept in germ-free flexible film isolators (Class Biologically Clean Ltd) at Rockefeller University. For wounding experiments, GF C57BL/6 mice were exported to isocages bioexclusion system (Tecniplast, PA, USA) and housed in isocages for the duration of the experiment. Wounding of GF mice was performed in a sterile hood using sterile autoclaved instruments. Wounding of specific-pathogen-free (SPF) C57BL/6 WT mice was performed in the same hood after GF mice were transferred into the isocages. Both GF and SPF mice were then housed in the isocages under the same conditions for 1 or 5 days as described before harvesting skin wounds. Mice housed in the isocages were provided with autoclaved food and water.
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