Control mirna

Manufactured by Bioneer

Control miRNA is a laboratory reagent used as a reference or control sample in experiments involving microRNA (miRNA) analysis. Its core function is to serve as a standardized sample to validate and normalize miRNA quantification data.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions) Smad2 3103, by Cell Signaling Technology (1 mentions) G9545, by Merck Group (1 mentions) Scramble sirna, by Bioneer (1 mentions) Sc 81189, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Control sirna, by Bioneer (1 mentions)

5 protocols using control mirna

miR-150 Mimics and Inhibitors Transfection

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HeLa cells were transfected with 100 nM hsa-miR-150-5p mimics (miR-150 mimics), 100 nM hsa-miR-150-5p inhibitors (miR-150 inhibitors), or 100 nM miRNA control (Bioneer) for 72 h using Lipofectamine RNAiMAX Transfection reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

Oboshi W., Hayashi K., Takeuchi H., Ikeda K., Yamaguchi Y., Kimura A., Nakamura T, & Yukimasa N. (2020). MicroRNA-150 suppresses p27Kip1 expression and promotes cell proliferation in HeLa human cervical cancer cells. Oncology Letters, 20(5), 210.

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Quantification of Smooth Muscle Markers

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Human recombinant platelet derived growth factor-BB (PDGF-BB) was purchased from Sigma (St. Louis, MO, USA). MiRNA-18a-5p and the miRNA control were purchased from Bioneer Co. (Daejeon, Korea).
Gapdh (sc-32233), syndecan4 (sc-15350), smooth muscle (SM) 22α (sc-373928), and SM α-actin (sc-130617) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Smad2 (#3103) was purchased from Cell Signaling Technology (Danvers, MA, USA).

Kee H.J., Kim G.R., Cho S.N., Kwon J.S., Ahn Y., Kook H, & Jeong M.H. (2014). miR-18a-5p MicroRNA Increases Vascular Smooth Muscle Cell Differentiation by Downregulating Syndecan4. Korean Circulation Journal, 44(4), 255-263.

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miR-134-5p Regulation of RUNX2

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miR‐134‐5p mimic, control miRNA, HDAC5 siRNA and scramble siRNA were purchased from Bioneer Corp. Antibodies against RUNX2 (23981, Abcam) and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (G9545, Sigma) were used at 1:1000 dilution.

Choe N., Shin S., Joung H., Ryu J., Kim Y., Ahn Y., Kook H, & Kwon D. (2020). The microRNA miR‐134‐5p induces calcium deposition by inhibiting histone deacetylase 5 in vascular smooth muscle cells. Journal of Cellular and Molecular Medicine, 24(18), 10542-10550.

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siRNA Design and Synthesis for circSmoc1-2

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The small interfering RNAs against circSmoc1-2 were produced using two different tools, the siDesign center from Horizon Discovery (https://horizondiscovery.com/design-center/) and the i-Score designer (https://www.med.nagoya-u.ac.jp/neurogenetics/i_Score/i_score.html).⁴⁷ (link) The siRNAs against circSmoc1-2 were designed to target the splicing junction, and siRNA against Adam19 mRNA for humans was constructed to base pair with exons within Adam19. These designed siRNAs were synthesized by Bioneer. The predesigned Adam19 siRNA for rat species, negative control siRNA, miR-874-3p mimic, and control miRNA were purchased from Bioneer. In addition, both the miR-874-3p inhibitor and negative miRNA inhibitor control were purchased from Qiagen.

Ryu J., Choe N., Kwon D.H., Shin S., Lim Y.H., Yoon G., Kim J.H., Kim H.S., Lee I.K., Ahn Y., Park W.J., Kook H, & Kim Y.K. (2021). Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells. Molecular Therapy. Nucleic Acids, 27, 645-655.

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Osteogenic Differentiation Regulatory Factors

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miR-27a-3p mimic and inhibitor, control miRNA, Atf3 siRNA, and control siRNA were purchased from Bioneer (Daejeon, Korea). Antibodies against Atf3 (sc-81189, Santa Cruz Biotechnology, CA, USA), Runx2 (23981, Abcam, Cambridge, UK), Alp (108337, Abcam), β-actin (47673, Cell Signaling Technology, Danvers, MA, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G9545, Sigma, St. Louis, MO, USA) were used at 1:1,000 dilution.

Choe N., Kwon D.H., Ryu J., Shin S., Cho H.J., Joung H., Eom G.H., Ahn Y., Park W.J., Nam K.I., Kim Y.K, & Kook H. (2020). miR-27a-3p Targets ATF3 to Reduce Calcium Deposition in Vascular Smooth Muscle Cells. Molecular Therapy. Nucleic Acids, 22, 627-639.

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