Diagnostic kit

Manufactured by Fujifilm

Fujifilm's diagnostic kits are laboratory equipment designed for the analysis and identification of various biological samples. These kits provide a standardized and reliable platform for healthcare professionals to conduct a range of diagnostic tests. The core function of the diagnostic kits is to facilitate the accurate detection and evaluation of specific analytes or biomarkers, enabling targeted medical decision-making.

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Lab products found in correlation

Diagnostic kits, by EKF Diagnostics (1 mentions) Wako kit, by Fujifilm (1 mentions)

Close spelling variants of this product

Wako Clinical Diagnostics kits Wako Diagnostics kits for Cholesterol E

4 protocols using diagnostic kit

Quantitating Liver Lipid Profile

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Aliquots of liver homogenate (5 mg protein from above) were also used for quantitating lipids including total cholesterol (TC), free cholesterol (C), triacylglycerol (TG), and phospholipid (PL) after solvent-extraction, thin layer chromatography (TLC), and quantitation as in (42 (link)). Alternately lipid classes were determined directly in the homogenate using diagnostic kits available from Wako Chemicals (Richmond, VA). Quantitation did not significantly differ between the two methods. To determine cholesteryl ester concentration (CE), free (non-esterified) cholesterol concentration (C) was subtracted from the total cholesterol. Serum β-hydroxybutyrate (β-Hydroxybutyrate LiquiColor, β-OHB) and high density lipoprotein cholesterol (Direct HDL-Cholesterol, HDL-C) were determined using diagnostic kits from Stanbio Laboratory (Boerne, TX). Serum apolipoprotein B (apoB) and apolipoprotein A-I (apoA1) were quantitated with diagnostic kits from Diazyme Labs (Poway, CA). Serum non-HDL-C was calculated by subtracting serum HDL-C from serum TC.

Landrock D., Milligan S., Martin G., McIntosh A., Landrock K.K., Schroeder F, & Kier A.B. (2017). EFFECT OF Fabp1/Scp-2/Scp-x ABLATION ON WHOLE BODY AND HEPATIC PHENOTYPE OF PHYTOL-FED MALE MICE. Lipids, 52(5), 385-397.

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Hepatic Lipid Profiling in Mice

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Portions of liver (0.1g wet weight) were homogenized in 0.5mL PBS (pH 7.4) using a motor-driven pestle (Tekmar Co, Cincinnati, OH) at 2000 rpm for 5 min. Liver homogenate, serum, and individual lipoprotein classes (LDL, HDL, VLDL) isolated from serum were analyzed for individual lipid classes using diagnostic kits (Wako, Richmond, VA) for total cholesterol (TC), free cholesterol (FC), triglyceride (TG), phospholipid (PL), and HDL cholesterol (HDL-C). Cholesteryl ester (CE) levels were calculated by subtraction of FC from TC. Serum non-HDL-C was determined by subtraction of serum HDL-C from serum TC. All diagnostic kits were used as per the manufacturer’s instructions, modified for use with 96-well plates and reader.

McIntosh A.L., Atshaves B.P., Martin G.G., Landrock D., Milligan S., Landrock K.K., Huang H., Storey S.M., Mackie J., Schroeder F, & Kier A.B. (2019). Effect of Liver Fatty Acid Binding Protein (L-FABP) Gene Ablation on Lipid Metabolism in High Glucose Diet (HGD) Pair-Fed Mice. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864(7), 985-1004.

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Triacylglycerol Measurement in Mouse Liver

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Frozen mouse liver (100–200 mg wet weight) was homogenized in 1.0 mL of ice-cold homogenization buffer as described earlier [28 ]. Triacylglycerol (TG) was measured in liver homogenate using a diagnostic kit from Wako Chemicals (Richmond, VA) as per the manufacturer’s instructions. Measuring triacyglycerol in liver homogenate with the Wako kit versus chemical analysis (i.e. solvent extraction, thin layer chromatography, elution and colorimetric measurement) revealed no significant differences between the two methods [29 (link)].

Martin G.G., Seeger D.R., McIntosh A.L., Chung S., Milligan S., Landrock D., Dangott L.J., Golovko M.Y., Murphy E.J., Kier A.B, & Schroeder F. (2018). Scp-2/Scp-x Ablation in Fabp1 Null Mice Differentially Impacts Hepatic Endocannabinoid Level Depending on Dietary Fat. Archives of biochemistry and biophysics, 650, 93-102.

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Hepatic Lipid Fractions Extraction and Analysis

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Hepatic lipid fractions were extracted using Folch solution (a mixture of 1 vol. of methanol and 2 vol. of chloroform), as described previously^{14 (link)}. The amounts of TG, total cholesterol, and NEFA in liver and serum were then measured using colorimetric enzyme assays (Wako Pure Chemical Industries; Tokyo, Japan). Liver injury was assessed by measuring serum ALT and AST levels using a diagnostic kit (Wako Pure Chemical Industries).

Arai T., Tanaka M, & Goda N. (2018). HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver. Scientific Reports, 8, 14230.

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