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3 protocols using ethyl acetate etoac

1

LC-MS Quantification of Synthetic Drug DL0410

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DL0410 (purity >99%) was synthesized by the Institute of the Materia Medica, Chinese Academy of Medical Sciences (Beijing, China). Phenacetin (internal standard; IS) was obtained from the National Institutes for Food and Drug Control (Beijing, China). Acetonitrile (ACN; CH3CN; LC-MS-grade) and methanol (MeOH; LC-MS grade) were purchased from J.T. Baker; Thermo Fisher Scientific, Inc., (Waltham, MA, USA). Formicacid [HCOOH; high (H)PLC-grade] was obtained from Tedia (Fairfield, CT, USA). Recombinant CYP450s (CYP1A2, CYP2C9, CYP2D6 and CYP3A4) were obtained from Corning Life Sciences (Tewksbury, MA, USA). Potassium phosphate buffer (pH 7.4) was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Ethyl acetate (EtOAc) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Anticoagulation tubes with heparin were obtained from Jiangsu Kangjian Healthcare Co., Ltd., (Taizhou, China). Pure water was purchased from the Hangzhou Wahaha Company (Hangzhou, China), with all other chemical reagents being of analytical grade level or higher.
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2

Versatile Doxorubicin Nanocarriers Synthesis

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o-Nitrobenzyl alcohol (98%), N,N-diisopropylethylamine (DIPEA, 98%), triphosgene (98%), bis(trimethoxysilylpropyl)amine (98%) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX‧HCl) was purchased from Wuhan Dongkang Source Technology Co., Ltd. (Wuhan, China). Triethylamine (Et3N), hexane, dichloromethane (DCM), petroleum ether (PE), tetrahydrofuran (THF), ethyl acetate (EtOAc), isopropyl alcohol (i-PrOH), and methanol (MeOH) were sources from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). All organic solvents were distilled over a suitable drying reagent before use. All other reagents were analytical reagent grade and were purchased from Tianjin fuyu fine chemical Co., Ltd. (Tianjin, China).
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3

Characterization of Plastic Materials

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The PC commodities (pellet, bucket, veil, goggle, disc, tube), polyethylene terephthalate (PET) bottle materials, and AcrylonitrileButadieneStyrene copolymer (ABS) granular material were purchased on Taobao.com or Amazon.cn and used without further purification. N,N′-dimethyl-ethylenediamine (DMEDA) (98%, Macklin, Shanghai, China), 1,3-dimethyl-2-imidazolidinone (DMI) (>99%, Aladdin, Shanghai, China), and bisphenol A (99%, Macklin) were used without further purification. Dibromomethane (98%, Energy Chemical, Shanghai, China) and hexamethylbenzene (98%, Energy Chemical) were used as internal standards. Dichloromethane (DCM) (>99%), tetrahydrofuran (THF) (>99%), ethyl acetate (EtOAc) (>99%), toluene (Tol.) (>99%), and acetone (Ace.) (>99%) were purchased as solvents at Sinopharm Chemical Reagent Co. (Shanghai, China). Chloroform-d (>99%) was purchased at Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). Dimethyl sulfoxide-d6 (>99%) and benzene-d6 (>99%) were purchased at Energy Chemical. Chromatographic THF was purchased from Honeywell LTD (Tokyo, Japan) for the analysis of GPC measurements.
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