Yeast extract peptone dextrose ypd medium

The bacterial and fungal strains (Pseudomonas, Paenibacillus, Aspergillus, Saccharomyces, and Schizosaccharomyces) used in this study are listed in Table 2. For routine culture, Pseudomonas and Paenibacillus cells were grown in liquid LB medium or on LB agar plates at 30°C. Aspergillus nidulans wild-type and mutant strains were cultured in supplemented minimal medium at 28°C overnight in chambered cover glasses (20 (link)). Yeast strains were grown in yeast extract-peptone-dextrose (YPD) medium (Sigma-Aldrich, St. Louis, MO, USA) or on YPD agar (Sigma) plates at 30°C. An orbital shaker (600 rpm) was used for liquid cultures.

Candida albicans (900228), C. parapsilosis (90018), C. krusei (6258), and C. glabrata (90030) were purchased from the American Type Culture Collection (ATCC), C. lipolytica was kindly provided by Andreas Sonesson (Lund University), and the SKCA23-ACTgLuc strain was the gift of Patrick Van Dijck (VIB-KU Leuven). All yeast species were maintained by subculturing every 3 weeks on Sabouraud dextrose agar (SDA; Sigma-Aldrich, St. Louis, MO) plates. Before each experiment, one colony of yeast was inoculated in 5 ml of yeast extract-peptone-dextrose (YPD) medium (Sigma-Aldrich) and allowed to grow overnight at 29°C. The day after, the optical density at 620 nm (OD₆₂₀) was measured using a spectrophotometer (Genesys 20; Thermo Scientific, Rochester, NY). When the culture had reached the mid-logarithmic phase, i.e., ∼0.6 OD, the medium was removed by centrifuging the culture at 5,600 × g (1-6P; Sigma, USA) for 10 min. The pellet was resuspended in 5 ml of 10 mM Tris at pH 7.4 and centrifuged once more. After washing, the pellet was resuspended in 10 mM Tris at pH 7.4 (0.6 OD/ml) to obtain a 1% solution of yeast, which corresponds to approximately 1 × 10⁹ CFU/ml.