Tissues harvested from mice were placed in 4% formalin, followed by 70% alcohol and PBS before embedding. Tissues were then embedded in paraffin, sectioned, and stained with hematoxylin and eosin. Tissue sections were evaluated by a board-certified pathologist (M.C.). Images were visualized using an Olympus Vanox AHBS3 microscope with an Olympus SPlan Apo × 20/ 0.70 NA objective (Olympus, Woodbury, NY). A diagnostic instrument spot RT color digital camera utilizing Spot software version 4.0.2 was used to acquire the images (Diagnostic Instruments, Sterling Heights, MI). Murine immunohistochemistry was performed as previously described (DuPage et al., 2011 (link)). Tumor tissues were fixed in 4% paraformaldehyde, processed, embedded in paraffin, then cut into 5 mm sections. Paraffin sections were blocked with 3% hydrogen peroxide solution (Sigma Cat H1009), vector streptavidin/biotin (Vector Laboratories cat. SP-2002), and CAS-Block protein block (ThermoFisher Cat. 008120), then stained with CD8 antibody (Biorbyt Cat orb10325). For human immunohistochemistry, blocks were obtained from clinical trials and cut into different sections. Sections were then duo-stained with CD8 antibody (Dako Cat. M7103) and MART-1 antibody (Novus Biotechne Cat. NBP1–30151) using the Envision G2 Doublestain Kit (Dako Cat. K5361).
Cd8 antibody
Manufactured by Biorbyt
Sourced in United States
The CD8 antibody is a laboratory reagent used for the detection and identification of CD8-positive cells, a subset of T lymphocytes. The CD8 molecule is a co-receptor that assists the T cell receptor in the recognition of antigen-major histocompatibility complex class I complexes. The CD8 antibody can be used in various immunological techniques to study the function and distribution of CD8-positive cells.
Automatically generated - may contain errors
2 protocols using cd8 antibody
1
Murine and Human Tissue Immunohistochemistry
2
Hepatoma Tissue Immunohistochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunohistochemical analysis, paraffin‐embedded hepatoma tissue blocks were sectioned into 3‐μm‐thick slices and mounted on poly(lysine)‐coated slides. The slides were deparaffinized, blocked with 3% hydrogen peroxide for 10 min, and then subjected to antigen retrieval in 10 mM citrate buffer for 15 min in a microwave. The slides were incubated with CD31, CD44, CD133, and MDR‐1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA); Ki‐67 and PD‐L1 antibody (Thermo Fisher Scientific, Waltham, MA, USA); CD68 antibody (clone ED‐1) (EMD Millipore, Temecula, CA, USA); FOXP3 antibody (Bioss Antibodies, Woburn, MA, USA); and CD8 antibody (Biorbyt, Cambridge, UK), at 4°C overnight. After the sections were washed with PBS, they were incubated with horseradish peroxidase/Fab polymer conjugate (polymer detection system; Zymed Laboratories, South San Francisco, CA) for 30 min and detected using diaminobenzidine (1:20 dilution; Zymed Laboratories). TUNEL analysis was performed using the in situ Cell Death Detection Kit, Fluorescein (Roche, Indianapolis, IN), according to the manufacturer's protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!