Ft ir 610 spectrometer

Optical microscopy investigation was effectuated using a transmitted light biological microscope (ZEISS Axioscope 5, Oberocken, Germany).
Scanning Electron Microscopy was effectuated using a Hitachi SU8230 Scanning Electron Microscope, Tokyo, Japan, at an acceleration voltage of 30 kV in low-vacuum mode without sample metallization. Elemental analysis was effectuated with an Energy-Dispersive Spectroscopy (EDS) detector: X-Max 1160 EDX (Oxford Instruments, Oxford, UK). The culture medium within wells was removed before SEM investigation and afterwards rinsed with ultrapure water and stored under a desiccator for 1 h until their surface was completely dry.
Fourier-transform infrared spectroscopy (FTIR) was performed using an FTIR 610 spectrometer (Jasco Corporation, Tokyo, Japan) in the wavenumber range of 4000–400 cm⁻¹, using the potassium bromide pellet technique. Each spectrum was registered at a resolution of 4 cm⁻¹ and represents the average of 100 scans.

All chemicals and solvents were of commercial reagent quality and were used without further purification unless otherwise stated. DMA was dried over molecular sieves of size 4 Å and distilled under reduced pressure. TEOA was distilled under reduced pressure. Tetraethylammonium tetrafluoroborate was dried in vacuo at 100°C overnight before use. [Ir(piq)₂(BL)](PF₆) (Kuramochi and Ishitani, 2016 (link)), BNAH (Mauzerall and Westheimer, 1955 (link)), BI(OH)H (Hasegawa et al., 2005 (link); Zhu et al., 2008 (link)), Ru (Anderson et al., 1995 (link)), and BL (Sun et al., 1997 (link)) were synthesized according to literature procedures. ¹H NMR spectra were recorded on an AL400 NMR spectrometer. IR spectra were measured in dichloromethane on a JASCO FT/IR-610 spectrometer. Electrospray ionization–mass spectroscopy (ESI-MS) was undertaken using a SHIMADZU LCMS-2010A system with acetonitrile as a mobile phase. UV–vis absorption spectra were recorded with a JASCO V-670 instrument. Emission spectra were measured at 25°C under an Ar atmosphere using a JASCO FP-8600 spectrofluorometer with correlation for the detector sensitivity. Emission quenching experiments were performed in DMA or DMA/TEOA (5:1 v/v) solutions containing a complex and several different concentrations of BNAH or BI(OH)H.