We purchased wild type C57BL/6 mice (male, 10-week-old, 25–30 g on arrival) from Charles River Laboratories (Wilmington, MA). The mice were housed and kept in a colony room with food and water available ad libitum. The colony room was maintained on a 12:12 light to dark cycle and ambient temperature at 23.0 °C ± 1.5 °C. All procedures were conducted in accordance with the National Institutes of Health’s Guide for the Care and Use of Experimental Animals and were approved by Eastern Virginia Medical School’s Institutional Animal Care and Use Committee (protocol number: 20-010). The CRHR2 specific antagonist, As-2B, was purchased from Tocris (catalog# 2391).
As 2b
Manufactured by Bio-Techne
The As-2B is a laboratory equipment product designed for general use in research and analytical environments. It serves as a centrifuge, providing a core function of separating components within liquid samples through the application of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using as 2b
1
Behavioral Characterization of C57BL/6 Mice
2
Pharmacological Modulation of Addiction-Related Behaviors
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Methamphetamine hydrochloride (METH: 2mg/kg, IP; Sigma-Aldrich) or cocaine hydrochloride (COC: 15mg/kg, IP; in the mini-pump, the concentration was 22.5mg/ml or 11.25mg/ml; NIDA) were dissolved in sterile 0.9% saline and administered at the designated doses. In some experiments, mice also received injections (IP) of vehicle (0.9% DMSO/25% hydropropylβ-cyclodextrin; HPβCD) or racemic (+/−) Blebbistatin (blebb; Tocris). Blebb was diluted to 1mg/ml in HPβCD vehicle, and administered at the dose of 10mg/kg, as previously described [4 (link),6 (link),8 (link)]. In some experiments, vehicle (sterile saline) or the selective CRF2 antagonist Astressin-2B [AS2B, Tocris; 20 (link),21 (link),22 (link)] was infused into BLA (1ug/0.5ul/side at a rate of 0.25ul/minute) 20 minutes before conditioning, or 15 minutes after the final conditioning session. AS2B dose, rate, and times of infusions were based on previous research [23 (link)–25 (link)]. Additionally, in other experiments, vehicle (sterile saline) or the selective CRF2 agonist Urocortin 3 (UCN3, Bachem) was infused into BLA (2ug/0.66ul/side at a rate of 0.3ul/minute) 15 minutes after the final conditioning session or 20 minutes before each PM conditioning session (CS+). UCN3 dose and rate were based on previous research [26 (link)–29 (link)].
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