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Triglyceride content assay kit

Manufactured by Solarbio
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The Triglyceride content assay kit is a laboratory tool used to quantify the amount of triglycerides present in a given sample. It provides a standardized method for the analysis of this important metabolic marker.

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Lab products found in correlation

Bca protein concentration assay kit, by Beyotime (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Anti occludin antibody, by Abcam (1 mentions) Oil red o, by Merck Group (1 mentions) Superreal premix plus sybr green, by Tiangen Biotech (1 mentions) Vitamin d3, by Solarbio (1 mentions) Mouse tnf alpha elisa kit, by RayBiotech (1 mentions) Mouse il 6 elisa kit, by RayBiotech (1 mentions) Total cholestenone content assay kit, by Solarbio (1 mentions) Total sod activity detection kit, by Beyotime (1 mentions) Anti fas antibody, by Abcam (1 mentions) Anti zo1 tight junction protein antibody, by Abcam (1 mentions) Cdna synthesis kit, by Tiangen Biotech (1 mentions) Hdl and ldl vldl cholesterol assay kit, by Abcam (1 mentions) Total cholesterol assay kit, by Nanjing Jiancheng (1 mentions) A111 1 1, by Nanjing Jiancheng (1 mentions)

6 protocols using triglyceride content assay kit

1

Metabolite Quantification Protocol

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The levels of glucose, lactate, NADH and NAD + were measured using commercial assay kits (Beyotime Biotechnology) following the manufacturer’s instructions. The triglyceride (TG) content was measured using a Triglyceride Content Assay Kit (Solarbio Life Science, China).
Jian L., Gao X., Wang C., Sun X., Xu Y., Han R., Wang Y., Xu S., Ding L., Zhou J., Gu Y., Zhao Y., Yang Y., Yuan Y., Ye J, & Zhang L. (2023). Perilipin 5 deficiency aggravates cardiac hypertrophy by stimulating lactate production in leptin-deficient mice. Biology Direct, 18, 54.
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2

Triglyceride Content Determination in Adipocytes

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The cellular triglyceride content was determined by the triglyceride content assay kit (BC0625, Solarbio, China). Differentiated 3T3-L1 adipocytes were lysed by reagent I and sonicated for 1 min. After centrifugation (8,000g for 10 min at 4°C), the supernatant was collected for triglyceride determination according to the instruction of the manufacturer.
Tao J., Yu X.L., Yuan Y.J., Shen X., Liu J., Gu P.P., Wang Z., Ma Y.T, & Li G.Q. (2022). DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse Model. Frontiers in Endocrinology, 12, 723623.
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3

Investigating Liver Inflammation and Metabolism

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Vitamin C (Tianjin Kingyork, H12020392), Vitamin D3 (Beijing Solarbio Science & Technology, V8070), Mouse TNF-alpha ELISA Kit (RayBiotech, P06804), Mouse IL-6 ELISA Kit (RayBiotech, P08505), Mouse IL-1β ELISA Kit (Shanghai Tongwei Biotechnology, TW56961), Mouse insulin ELISA Kit (Shanghai Tongwei Biotechnology, TW085566), Mouse endotoxin (ET) ELISA Kit (Jiangsu Meimian, MM-0369M1), Total Cholestenone Content Assay Kit (Beijing Solarbio Science & Technology, BC 1985), Triglyceride Content Assay Kit (Beijing Solarbio Science & Technology, BC0625), Total SOD activity detection kit (Shanghai Beyotime Biotechnology, S0101S), Lipid oxidation detection kit (Shanghai Beyotime Biotechnology, S0131S), BCA protein concentration assay kit (Shanghai Beyotime Biotechnology, P0010), Oil red O (Sigma, O0625), Anti-FXR1 antibody (Abcam, ab155124), BSEP Monoclonal antibody (Proteintech Group, 67512-1-Ig), Anti-Fas antibody (Abcam, ab271016), Anti-ZO1 tight junction protein antibody (Abcam, ab221547), Anti-Occludin antibody (Abcam, ab222691), Anti-SLC10A2/ASBT antibody (Abcam, ab203205), TRIzol Reagent (Thermo Fisher, 15596026), cDNA synthesis kit (Beijing Tiangen Biotech, KR118), and SuperReal PreMix Plus (SYBR Green) (Beijing Tiangen Biotech, FP205) were used.
Chen Q., Zhao L., Mei L., Zhao X., Han P., Liu J., Meng C., Li R., Zhong R., Wang K, & Li J. (2023). Vitamin C and vitamin D3 alleviate metabolic-associated fatty liver disease by regulating the gut microbiota and bile acid metabolism via the gut-liver axis. Frontiers in Pharmacology, 14, 1163694.
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4

Triglyceride Content Assay in OMECs

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For the OMECs transfected with the miR-199a-3p mimic, miR-199a-3p inhibitor, miR-199a-3p mimic NC, and miR-19a-3p inhibitor NC, the supernatant was collected by centrifugation from 300 μl of lysate solution added 48 h after transfection. The content of triglycerides in OMECs was measured in quadruplicate using a triglyceride content assay kit (Solarbio, Beijing, China) by a microplate reader (Thermo Lifetech, MA, USA).
Wang J., Hao Z., Hu L., Qiao L., Luo Y., Hu J., Liu X., Li S., Zhao F., Shen J., Li M, & Zhao Z. (2022). MicroRNA-199a-3p regulates proliferation and milk fat synthesis of ovine mammary epithelial cells by targeting VLDLR. Frontiers in Veterinary Science, 9, 948873.
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5

Biochemical Markers Quantification

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The levels of urinary albumin, serum creatinine, and blood urea nitrogen were measured using the corresponding assay kits following the manufacturer's instructions (Jiancheng Biological Technology Institute). Plasma triglyceride and total cholesterol levels were determined using the triglyceride content assay kit (BC0620, Solarbio) and the total cholesterol assay kit (A111–1–1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively. The contents of high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL)/very-low-density lipoprotein (VLDL) cholesterol were detected using the HDL and LDL/VLDL cholesterol assay kit (ab65390, Abcam).
Zhang Z., Fu X., Zhou F., Zhang D., Xu Y., Fan Z., Wen S., Shao Y., Yao Z, & He Y. (2024). Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy. Journal of Diabetes Research, 2024, 6942156.
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6

Lipid Profile Biochemical Indicators

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Blood biochemical indicators of the lipid profile were assessed. The concentrations of total cholesterol (TC) were measured by Micro total cholesterol (TC) content assay kit (Solarbio, Beijing, China) and triglyceride (TG) was assessed by triglyceride content assay kit (Solarbio, Beijing, China) according to the manufacturer’s instructions.
Zhang X., Yang G., Chen Y., Mu Z., Zhou H, & Zhang L. (2022). Resveratrol pre-treatment alleviated caerulein-induced acute pancreatitis in high-fat diet-feeding mice via suppressing the NF-κB proinflammatory signaling and improving the gut microbiota. BMC Complementary Medicine and Therapies, 22, 189.
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