The HPLC instrument was a 1290 series HPLC system (Agilent, Santa Clara, CA, USA), which comprised a pump (G4220A), an autosampler (G4226A), and a thermostatted column compartment (G1316C). An API 4000 triple quadrupole system (Sciex, Concord, ON, Canada) was employed as the mass spectrometric detector. The data were processed using the Analyst® 1.6.2 software.
Api 4000 triple quadrupole system
Manufactured by AB Sciex
Sourced in Canada
The API 4000 triple quadrupole system is a highly sensitive and precise mass spectrometry instrument designed for quantitative and qualitative analysis. It features two quadrupole mass analyzers and a collision cell, enabling multiple-reaction monitoring (MRM) and enhanced selectivity for accurate and reliable results.
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Lab products found in correlation
2 protocols using api 4000 triple quadrupole system
1
HPLC-MS/MS Analytical Platform
2
Ovarian Steroid Quantification by LC-MS/MS
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Ovaries were isolated 8.5–9 h before the onset of light and immediately frozen with liquid nitrogen. Collected samples were stored at −80 °C until analysis. Ovarian 17β-estradiol and 17α,20β-DHP content was measured by LC-MS/MS. Lipids were extracted using tert-butyl methyl ether and purified using Oasis MAX (Waters). Lipids were derivatized to picolinyl esters by a reaction with picolinic acid for the detection of poorly ionized steroids, especially those of low abundance. Analytes were separated by the Nexera UPHPLC system (Shimadzu) and detected with an API 4000 triple quadrupole system (Sciex). Positive electrospray ionization mode was used. Lipid extraction and analysis by LC-MS/MS were performed at Aska Pharma Medical.
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