Znso4 7h2o

Wheat bran/wheat flour (Roland Mills Nord GmbH & Co. KG, Bremen, Germany); H₂O₂ (Sigma Aldrich, Munich, Germany); HNO₃ (Sigma Aldrich, Munich, Germany); Indium Standard for ICP-MS TraceCERT (Sigma Aldrich, Munich, Germany); Multielement Standard Solution 6 for ICP TraceCERT (Sigma Aldrich, Munich, Germany); Rhodium ICP-MS Standard TraceCERT (Sigma Aldrich, Munich, Germany); ZnSO₄∙7H₂O (Sigma Aldrich, Munich, Germany).

Three hair dyes; Arianor Madder red 306003 (R), Arianor Straw Yellow 306005 (Y), Arianor Ebony 306020 (E), and ZnSO₄.7H₂O were purchased from Sigma Aldrich (Darmstadt, Germany) and used without further purification. Three stock solutions of 1000 mg·L⁻¹ concentrations were prepared and then diluted to the required working concentrations (25–150 mg·L⁻¹). The pH of solutions was adjusted using 0.1 M NaOH and 0.1 M HCl solutions. The functional groups on the oak cupule powder, O, and oak coated with ZnO, COZ, before and after the adsorption processes were characterized utilizing a TENSOR FTIR instrument from BRUKER. The FT-IR measurements were taken within the wavenumber region of 500–4000 cm⁻¹. Adsorbent material was portrayed utilizing scanning electron microscopy (SEM; Model: A Phenom XL G2 scanning electron microscope from Thermo Fisher Scientific, Waltham, MA, USA). With the use of the PANalytical B. V. Lelyweg 17602 EA ALMELO instrument, X-ray diffraction (XRD) investigations were conducted.

To detect the assimilation of selected neurotransmitters, we used a modified M9 minimal medium (pH 7.4) [43 ], consisting of 0.6% Na₂HPO₄ (Sigma Aldrich, USA), 0.3% KH₂PO₄ (Sigma-Aldrich, USA), 0.05% NaCl (Fisher Scientific, USA), 0.1% NH₄Cl (Merck, Germany), and 0.15% agar in Milli-Q water. To the autoclaved and cooled medium were added 5 mL of 40% (w/v) glucose, and 1 mL of microelements [3 mM (NH₄)₆Mo₇O₂₄ × 4H₂O (Sigma Aldrich, USA), 400 mM H₃BO₃ (Merck, Germany), 30 mM CoCl₂ × 6H₂O (Merck, Germany), 10 mM CuSO₄ × 5H₂O (Merck, Germany), 80 mM MnCl₂ × 4H₂O (Sigma Aldrich, USA), 10 mM ZnSO₄ × 7H₂O (Sigma Aldrich, USA), 1 mL of 1 M MgSO₄ (Carlo Erba, Milano, Italy), 100 µL of 1 M CaCl₂ (Gram-mol, Zagreb, Croatia), and 200 µL of 5 mM FeSO₄ (Sigma Aldrich, USA)]. After autoclaving, we added filter-sterilized 0.1 M of one of the selected neurotransmitters: acetylcholine (ACh), γ-aminobutyric acid (GABA), glycine (Gly), glutamate (Glu), and dopamine (DA) (all Sigma-Aldrich, USA). M9 minimal medium (pH 7.4) without added neurotransmitters was used as a control. Plates were inoculated with 10 µL of cell suspension or plugs of mycelium in three replicates and incubated for one month at 24 °C and 37 °C. Assimilation was assessed by comparing growth on plates with and without added neurotransmitters.

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 1-aminobeznotriazole (ABT) were obtained from Sigma-Aldrich (Germany). Stock solutions of ABT were prepared in ethanol at a concentration of 50 mM.
o-tolidine was purchased from Chempur (Poland). A stock solution of o-tolidine (10 mg mL^-1) was prepared in ethanol. Liquid chromatography solvents were obtained from Avantor Performance Materials (Poland).
The azo dyes Reactive Orange 16 (CAS 12225–83-1), Acid Red 27 (CAS 915–67-3), Acid Orange 7 (CAS 633–96-5), Reactive Red 120 (CAS 61951–82-4), and Reactive Black 5 (CAS 17095–24-8) were purchased from Sigma-Aldrich (Germany). Stock solutions were prepared in water at a concentration of 50 mg mL^-1.
Cd(NO₃)₂ × 4 H₂O, K₂Cr₂O₇, and ZnSO₄ × 7 H₂O were purchased from Sigma-Aldrich (Germany). Metal stock solutions were prepared by dissolving in deionized water at a concentration of 1 M.
Samples of landfill leachate (L1 and L2) collected from the landfill of the former “Boruta” Dye Industry Plant in Zgierz, Poland (Fig. 1) were kindly supported by the Voivodeship Inspectorate of Environmental Protection in Łódź, Poland.

ZnSO₄.7H₂O (>99.0%), Li₂SO₄·H₂O (>99.0%), and ethylene diamine tetraacetic acid disodium salt (EDTA-2Na, A.R.> 99.0%) were prepared from Sigma-Aldrich Chemical Co. All reagents were used directly without further purification. All aqueous electrolytes used deionized water as the solvent.

CellMask™DeepRed (ThermoFisher Scientific, Waltham, MA, USA); Cy3^® goat anti rabbit IgG (Jackson ImmunoResearch, Dianova, Hamburg, Germany); Chelex^® 100 Resin (Bio-Rad, Hercules, CA, USA); Dulbecco’s modified Eagles medium (DMEM) (PAN-Biotech, Aidenbach, Germany); Hoechst 33258 (Sigma Aldrich, Munich, Germany); fluorescein isothiocyanate (FITC)-dextran 20 (TdB, Uppsala, Sweden); fetal calf serum (FCS) (CCPro, Oberdorla, Germany); mucin from porcine stomach Type II (Sigma Aldrich, Munich, Germany); PAR (Sigma Aldrich, Munich, Germany); Transwell inserts (Corning, New York, NY, USA); N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) (Sigma Aldrich, Munich, Germany); WillCo-dish^® glass bottom dish (WillCo, Amsterdam, The Netherlands); Zincon (Sigma Aldrich, Munich, Germany); ZnSO₄·7H₂O (Sigma Aldrich, Munich, Germany). All other chemicals were purchased from standard sources.