This investigation was performed by injecting ISG through a 1-mL syringe through an 18-Guage needle into PBS (pH 6.8). The images indicate the gradual solution–gel transformation as a turbid layer occurred with time after the aqueous phase of surrounding agarose diffused to induce the phase separation of the formula. This transformation was recorded at different time intervals (i.e., 1, 5, 10, 15, and 30 min). The microscopic aspect of gel formation was investigated by injecting 150 µL samples into the agarose well (diameter of 7 mm). This well of agarose gel represents the crevicular pocket in humans. The change of this cross-sectional view of IBU matrix-like morphology is photographed under a stereo microscope (SZX10, Olympus Corp., Tokyo, Japan) at 0, 1, 5, 10, 20, and 30 min with SZX10 Series software.4.3.5.
Szx10
Manufactured by Olympus
Sourced in Japan, United States, Germany, China
The SZX10 is a stereo zoom microscope designed for a wide range of laboratory applications. It offers continuous zoom magnification from 8x to 80x, allowing users to observe specimens in detail. The SZX10 features a high-performance optical system and a compact, ergonomic design to provide clear, high-resolution images.
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Lab products found in correlation
Cellsens dimension desktop, by Olympus (4 mentions)
Cellsens software, by Olympus (3 mentions)
Eos 7d, by Canon (3 mentions)
Nova nanosem 450, by Thermo Fisher Scientific (3 mentions)
Msp 1s, by Hitachi (2 mentions)
Agar gel, by Nacalai Tesque (2 mentions)
Concanavalin a (cona), by Merck Group (2 mentions)
E 410, by Olympus (2 mentions)
Tm 1000, by Hitachi (2 mentions)
Femtojet 4i, by Eppendorf (2 mentions)
Femtotips 2, by Eppendorf (2 mentions)
Eclipse e400, by Nikon (2 mentions)
T 3400 sem, by Hitachi (2 mentions)
Lmp agarose, by Promega (2 mentions)
Szx16, by Olympus (2 mentions)
Uc50 camera, by Olympus (2 mentions)
Cellsens imaging software, by Olympus (2 mentions)
E 330, by Olympus (2 mentions)
S 3400n sem, by Hitachi (2 mentions)
Smz1500, by Nikon (2 mentions)
258 protocols using szx10
1
Monitoring In-Situ Gel Formation
2
Comprehensive Invertebrate Parasite Identification
3
Granulometric Analysis and Tensile Testing of ZnO Powder
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The granulometric composition analysis of the ZnO powder involved the utilization of the laser analyzer Fritsch Analysette 22, manufactured by FRITSCH GmbH in Germany. This analysis was conducted following the dispersion of the powder in isopropanol. Surfaces of produced and tested samples were observed with the help of digital microscope Keyence VHX-2000 (Keyence Ltd., Osaka, Japan) and scanning electron microscope (SEM) TESCAN VEGA (high vacuum mode was selected) and OLYMPUS SZX10 (Olympus Corporation, Tokyo, Japan). Tensile tests of manufactured specimens were performed with the help of tensile testing machine Zwick/Roell Z150 (ZwickRoell GmbH & Co. KG, Ulm, Germany). The applied loading speed was 5 mm·min−1. The reference specimens (0 wt.%), fixed in the Zwick/Roell Z020 (ZwickRoell GmbH & Co. KG) tensile testing machine, were subjected to the same loading parameters. The recorded data underwent analysis utilizing the TestXpert software (ZwickRoell GmbH & Co. KG, Ulm, Germany, version II). This software facilitated the determination of the modulus of elasticity by extracting it from the slope of the stress-strain curve within the linear region. The dimensions of specimens were measured with the help of the micrometer screw gauge (±0.005 mm).
4
Myxobacterial Predation Assay on P. aeruginosa
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A predation assay was performed on TPM plates (10 mM Tris-HCl (pH 7.6), 1 mM KPO4 pH 7.6, 8 mM MgSO4, 1.5% agar) using the colony-induced predation method as described [36 (link),37 (link)]. Overnight cultures of P. aeruginosa PAO1 were resuspended in an LB medium and cultured to the mid-exponential phase. The cells were collected, washed twice with MMC buffer (10 mM MOPS pH 7.6, 4 mM MgSO4, 2 mM CaCl2), and resuspended in MMC to OD550 nm = 50. In total, 100 µL of PAO1 cells was inoculated on TPM (1.5% agar) plates and allowed to dry. For monitoring the effect of different iron concentrations on myxobacterial predation (200 µM, 500 µM and 800 µM FeCl3) was added to the TPM (1.5% agar) plates. The M. xanthus strains were grown in CTT for 24 h, and the cells were in the exponential growth phases (OD550 nm ~0.6). The cells were collected by centrifugation and washed twice with MMC buffer and resuspended in MMC buffer to OD550 nm = 10 (1 × 1010 cells mL−1). Next, 3 µL of the cells was spotted at a 2 mm distance from the PAO1 colony. The plates were cultured at 30 °C for the indicated time. The lysed area of P. aeruginosa was observed with a stereomicroscope (Olympus SZX10, Olympus Corporation, Tokyo, Japan).
5
Zebrafish Embryo Development Assay
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All animal experimental procedures were done following approval from the Faculty of Medicine Institutional Animal Care and Use Committee, University of Malaya (ethics number: 2013-05-07/FAR/R/VI), and the animal care and use guidelines of this committee were followed. Adult zebrafish were maintained in a recirculating aquaculture tank (Zebtec; Tecniplast, Buguggiate, Italy) at 28°C with 14-/10-hour day/night light cycle. Embryos were obtained by natural spawning of adult zebrafish (4–8 months old). One hundred and eighty healthy embryos were selected at 24 hours postfertilization (hpf) and dechorionated manually. Embryos were then treated by incubating them in system water containing 20 μg/mL girinimbine for 24 hours. DMSO (1%) was used as negative control. Following 24-hour exposure, embryos were returned to system water for another 24 hours and monitored up to 72 hpf by stereomicroscopy (Olympus SZX10; Olympus Corporation) for viability and developmental abnormalities.
6
Predatory Microbe Interactions on Agar
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C. silvisoli c25j21 was inoculated on VY/2 agar plates (0.5% dried baker’s yeast, 0.1% CaCl2·2H2O, 1.5% agar) incubated at 30 °C for 5 days. Strain Curtobacterium flaccumfaciens GDMCC 1.343 and Pseudomonas syringae GDMCC 1.330 were inoculated in NB liquid medium and shaken at 160 rpm for 3 days at 30 °C. The freshly cultured pathogen was collected by centrifugation, washed, and resuspended in TPM buffer (10 mM Tris–HCl pH 7.6, 8 mM MgSO4, 1 mM KH2PO4) to adjust the optical density to OD600 = 5. Then a spot of 150 µL pathogen bacterial suspension was inoculated on the center of TPM agar (10 mM Tris–HCl-HCl pH 7.6, 8 mM MgSO4, 1 mM KH2PO4, 1.5% agar). A 5 mm diameter plug strain of c25j21 was inoculated on the center of the pathogen lawn and the plates were incubated at 30 °C. The zones of predation were observed with a stereomicroscope (Olympus SZX10, Olympus Corporation, Tokyo, Japan).
7
Characterization of Printed Materials
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The morphology of the specimens was observed by an optical microscope (OLYMPUS SZX10, Olympus Corporation, Tokyo, Japan) and a scanning electron microscope (SEM, JEOL JSM5510LV, Tokyo, Japan) equipped with energy dispersive X-ray spectroscopy (EDX, Oxford 7582, Oxfordshire, UK). The crystal structure was characterized by X-ray diffraction (XRD-6000 Cu-Ka radiation, Shimadzu, Tokyo, Japan). Thermogravimetric analysis and differential thermal analysis (DTA) (DTA-TG, Shimadzu DTG-60H, Shima, Tokyo, Japan) were used in the range from room temperature to 1400 °C in a flowing air atmosphere with a heating rate of 5 °C/min. The UV-vis-NIR spectra were obtained with a Cary 5000 UV-vis-NIR spectrophotometer. X-ray computed tomography (CT) scan of the printed part was carried out by using a high-resolution XCT system Phoenix Nanotom® m (GE Phoenix, Lewistown, PA, USA). The density was measured by the Archimedes’ method in water. To calculate the amount of shrinkage of the SLS parts, the dimension of cubic parts was measured by digital Vernier caliper.
8
Surface Characterization of PVC-P Membranes
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The surface condition of the collected PVC-P membrane Specimens was checked using an OLYMPUS SZX10 optical stereo microscope with the associated AnalySIS Auto hardware (Olympus Corporation, Tokyo, Japan). The thicknesses of the Specimens were also measured on a specially prepared Specimen containing pieces of all three PVC-P membranes Specimend, which were embedded in a Bakelite paste and the surfaces finely polished.
9
Scanning Electron Microscopy of Callitettix versicolor Antennae
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All Callitettix versicolor (Fabricius) specimens used in this study were collected in the Guizhou Province, China and preserved in 70% ethanol and stored at 4 °C.
Heads with antennae were separated from the rest of the body and rinsed in 70% ethanol in an ultrasonic cleaning. Dehydration was achieved with a graded ethanol series of 75%, 80%, 90%, and 95% each for 20 min and twice in 99.9% ethanol solutions for 30 min. The 99.9% ethanol was then substituted successively by mixing alcohol and tert-butyl ethanol with in proportions of 3:1, 1:1 and 1:3. Finally, specimens were placed in pure tert-butyl ethanol for 30 min. Specimens were dried in a freeze-drier (VFD-21S, SHINKKU VD, Tokyo, Japan) for 3 h. Thereafter, the antennae were removed from the dried samples under a stereomicroscope (Olympus SZX10, Olympus, Tokyo, Japan) and placed on aluminum stubs in various positions using double-sided sticky tape. The antennae were sputter-coated with gold-palladium (MSP-1S, Hitachi, Tokyo, Japan) and examined and observed at 15 kV in a Nova nano SEM450. Antennae of male and female adults were observed for comparison.
Heads with antennae were separated from the rest of the body and rinsed in 70% ethanol in an ultrasonic cleaning. Dehydration was achieved with a graded ethanol series of 75%, 80%, 90%, and 95% each for 20 min and twice in 99.9% ethanol solutions for 30 min. The 99.9% ethanol was then substituted successively by mixing alcohol and tert-butyl ethanol with in proportions of 3:1, 1:1 and 1:3. Finally, specimens were placed in pure tert-butyl ethanol for 30 min. Specimens were dried in a freeze-drier (VFD-21S, SHINKKU VD, Tokyo, Japan) for 3 h. Thereafter, the antennae were removed from the dried samples under a stereomicroscope (Olympus SZX10, Olympus, Tokyo, Japan) and placed on aluminum stubs in various positions using double-sided sticky tape. The antennae were sputter-coated with gold-palladium (MSP-1S, Hitachi, Tokyo, Japan) and examined and observed at 15 kV in a Nova nano SEM450. Antennae of male and female adults were observed for comparison.
10
Ultrastructural Analysis of Crustacean Tissues
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Intestines and hepatopancreases were removed from adult females (n = 5) under a stereomicroscope Olympus SZX10 using dissecting needles and micro forceps. Samples were immediately fixed in ice-cold 4% paraformaldehyde. Afterwards, samples were washed in phosphate buffered saline (PBS) (3x, 15 min each) and post-fixed in 1% aqueous osmium tetroxide for 2 h at room temperature. In-block staining with 2% aqueous uranyl acetate was performed overnight at room temperature, followed by washing in deionized water, dehydration in an ascending series of acetone (30–100%) and embedding in Durcupan resin (Honywell-Fluka, US). Semi-thin sections were cut to 0.5 μm, stained with 1% toluidine blue and inspected under a light microscope for orientation. Ultrathin sections were cut to 0.06-0.07 μm, placed on 100-mesh copper grids, stained with uranyl acetate and lead citrate according to Reynolds [52 (link)] and inspected under Jeol 1010 (Jeol, Japan) TEM operating at an accelerating voltage of 80 kV. Images were captured with a Mega View III camera (Olympus Soft Images Solutions GmBH, Germany) and assembled and annotated in Photoshop CS5 software (Adobe Systems, US).
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