For experiments investigating the effects of MSC administration, in vitro expanded and characterized MSCs were treated with either HGF‐specific small interfering RNA (siRNA) or control siRNA and injected into the tail veins of mice at 3 hours post‐transplantation (0.5 × 106 cells suspended in 100 μl of sterile saline) as previously described 11 , 14 , 23 . For experiments investigating the effects of HGF administration, 5 μl of either 0.1% murine recombinant HGF protein (R&D systems, Minneapolis, MN) in phosphate‐buffered saline (PBS) or protein control (mouse serum, Sigma‐Aldrich, St. Louis, MO) was applied topically to the ocular surface twice daily for 14 days following corneal transplantation.
Murine recombinant hgf protein
Manufactured by R&D Systems
Murine recombinant HGF protein is a laboratory reagent produced by R&D Systems. It is the mouse-derived version of the hepatocyte growth factor (HGF) protein. HGF is a multifunctional cytokine that plays a role in cell growth, motility, and morphogenesis.
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Lab products found in correlation
2 protocols using murine recombinant hgf protein
1
MSC and HGF Treatment Effects in Corneal Transplant
2
Evaluating HGF Penetration and Effects on LPS-Induced Corneal Haze
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To test the penetrance of HGF to the corneal epithelium, mice were topically treated with 3 µl of 0.1% His-tagged mouse recombinant HGF (His-Tag) (Sino Biological) following the second intrastromal injection of LPS. Intracorneal diffusion of HGF was confirmed with immunohistochemistry analysis using anti-his antibodies.
To evaluate the effect of HGF on LPS-induced corneal haze, mice were divided into two treatment groups: 0.1% murine recombinant HGF protein (R&D Systems). Mice were treated with 3 µl of HGF or control PBS, using a micropipette thrice daily for 5 days.
To evaluate the effect of HGF on LPS-induced corneal haze, mice were divided into two treatment groups: 0.1% murine recombinant HGF protein (R&D Systems). Mice were treated with 3 µl of HGF or control PBS, using a micropipette thrice daily for 5 days.
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