A UV/Vis/NIR spectrophotometer (LAMBDA 1050; Perkin Elmer, MA, USA) defined the transmittance of specimens (25 mm × 25 mm × 2 mm) for wavelengths between 200 to 1000 nm. Data was collected using UV WinLab software (LAMBDA 1050; Perkin Elmer, MA, USA).
Lambda 1050
Manufactured by PerkinElmer
Sourced in United States, United Kingdom, Germany
The Lambda 1050 is a high-performance UV-Vis-NIR spectrophotometer designed for a wide range of analytical applications. It features a dual-beam optical system and a broad wavelength range from 175 nm to 3300 nm, allowing for accurate and precise measurements across the ultraviolet, visible, and near-infrared spectrum.
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Lab products found in correlation
Nicolet 6700, by Thermo Fisher Scientific (9 mentions)
K alpha, by Thermo Fisher Scientific (8 mentions)
Escalab 250xi, by Thermo Fisher Scientific (4 mentions)
D8 advance, by Bruker (4 mentions)
S 4800, by Hitachi (4 mentions)
D max 2500, by Rigaku (3 mentions)
Invenio s, by Bruker (3 mentions)
Crossbeam 540, by Zeiss (3 mentions)
Nicolet is50, by Thermo Fisher Scientific (3 mentions)
Nicolet 6700 ft ir, by Thermo Fisher Scientific (3 mentions)
Sigma 300, by Zeiss (2 mentions)
Fluotime 300, by PicoQuant (2 mentions)
Nanolog spectrofluorometer, by Horiba (2 mentions)
Labram aramis raman microscope, by Horiba (2 mentions)
Smartlab, by Rigaku (2 mentions)
Spectrum one, by PerkinElmer (2 mentions)
X pert pro mpd, by Malvern Panalytical (2 mentions)
Jem 1400, by JEOL (2 mentions)
Empyrean x ray diffractometer, by Malvern Panalytical (2 mentions)
Helios nanolab 600, by Thermo Fisher Scientific (2 mentions)
168 protocols using lambda 1050
1
UV-Vis-NIR Spectroscopic Characterization
2
UV-Vis-NIR Spectroscopic Characterization
3
Quantifying Drug Encapsulation Efficiency
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Encapsulation efficiency is the quantity of drug captured in nanoparticles out of a certain quantity of drug added. It is percent efficiency of nanoparticles to encompass drug. Encapsulation efficiency was calculated using the following equations:
Briefly, 3 mg of the nanoparticles were dissolved in DMSO and investigated at 480 nm with the help of UV-Visible Spectrophotometer (LAMBDA 1050, PerkinElmer, USA). Concentration of doxorubicin was determined with the help of calibration curve. Calibration curve was drawn at different dilutions of doxorubicin (5–50 μg mL−1) using UV-Visible spectrophotometer (LAMBDA 1050, PerkinElmer, USA). Sample concentrations were plotted against the respective absorbance value. Straight line equation thus obtained from the graph was further utilized to determine drug concentration from the value of sample absorbance.
Briefly, 3 mg of the nanoparticles were dissolved in DMSO and investigated at 480 nm with the help of UV-Visible Spectrophotometer (LAMBDA 1050, PerkinElmer, USA). Concentration of doxorubicin was determined with the help of calibration curve. Calibration curve was drawn at different dilutions of doxorubicin (5–50 μg mL−1) using UV-Visible spectrophotometer (LAMBDA 1050, PerkinElmer, USA). Sample concentrations were plotted against the respective absorbance value. Straight line equation thus obtained from the graph was further utilized to determine drug concentration from the value of sample absorbance.
4
Comprehensive Material Characterization
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Microstructure and morphology were characterized by field emission scanning electron microscopy (SEM, Zeiss, Sigma 300, Jena, Germany). Optical absorbance spectra were obtained from a UV-Vis-NIR spectrophotometer with a 150 mm InGaAs integrating sphere (PerkinElmer, Lambda 1050S+, Shanghai, China). X-ray powder diffraction (XRD, Bruker D8 Advance, Karlsruher, Germany) was used to analyze the crystalline phase with the phase identification determined by using Cu Kα radiation. X-ray photoelectron spectroscopy (XPS, Thermo Fisher, ESCALab 250 Xi, MA, USA) with a monochromatic Al Kα source (1486.7 eV) was used to analyze the valence states of material elements at an operational pressure of 10−8 mBar, and the XPS spectrum was calibrated with the peak of carbon contamination C1 (284.8 eV). Inductively coupled plasma mass spectrometry (ICP-MS, ICAP RQ, MA, USA) was used to analyze the concentration of chromium element in the solution used for PEC measurements.
5
Optical and Structural Characterization of Coatings
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Optical total transmission spectra of the coatings on glass and diffuse reflection spectra of the samples on aluminum were both investigated using a UV-vis-NIR spectrophotometer (PerkinElmer Lambda 1050S), equipped with a spectralon-coated integrating sphere.
X-ray diffraction patterns were collected on a Bruker D5000 diffractometer working with Cu Kα radiation (λ = 0.154 nm, 40 kV, 40 mA).
Scanning electron microscopy (SEM) images were obtained on FEI Quanta200F equipped with EDX (EDAX genesis 4000) and on a JSM-IT500 LV (JEOL).
X-ray diffraction patterns were collected on a Bruker D5000 diffractometer working with Cu Kα radiation (λ = 0.154 nm, 40 kV, 40 mA).
Scanning electron microscopy (SEM) images were obtained on FEI Quanta200F equipped with EDX (EDAX genesis 4000) and on a JSM-IT500 LV (JEOL).
6
Liposome Fluorescence Characterization
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Liposomes (30 µL; 125 nmol of lipid) with pHrodo-DOPE were diluted in 2.7 mL transport buffer (20 mM MOPS-KOH, pH 7.0, 52.5 mM K 2 SO 4 ) with the pH value adjusted by addition of HCl. Absorption spectra were measured on a Lambda 1050 (PerkinElmer) with 2 nm slits and were corrected for scattered light by subtracting an absorption spectrum of a dye free sample. For the emission measurements pHrodo-DOPE and the reference dye were excited with a 509 nm pulsed laser with a laser power of 190 μW (model number P-C-510 PicoQuant GmbH) on a Fluotime 300 instrument (PicoQuant GmbH). All emission spectra were measured with a pulse repetition rate of 40 MHz and were corrected for the spectral response of the detector. Slit widths of 5 nm were used for all emission measurements. Time-correlated single photon counting experiments were conducted with the same 509 nm pulsed laser on a Fluotime 300 instrument. The pulse rate was 16 MHz and the emission was measured at 590 nm. Measurements were performed at 25 °C in a water thermostated cuvette holder. The fluorescence decays were fitted to a bi-exponential function. The full width at half-maximum (FWHM) of the instrument response function was typically in the order of 42 ps.
7
Optical Characterization of CsPbBr3 NPLs
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Samples for optical measurements in solution were prepared by diluting the stock solution of washed CsPbBr3 NPLs with hexane. Absorption spectra were measured on a double-beam PerkinElmer Lambda 1050 UV/vis spectrometer. For fast-spectroscopy measurements, solutions with an optical density 0.1 at 400 nm of CsPbBr3 NPLs and ~0.14 at 510 nm of CsPbBr3 NPLs + PDI hybrid were prepared (Supplementary Fig. 2 ). PL spectra were recorded on an Edinburgh Instruments FLS980 Spectro-fluorimeter equipped with a 450 W xenon lamp as excitation source and double grating monochromators.
8
Reflectance Spectroscopy of Etched Catalyst Films
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Total reflectance
spectroscopy was carried out using a PerkinElmer Lambda 1050 equipped
with a 150 mm integrating sphere and PbS and InGaAs detectors, collecting
both the diffuse and the specular reflectance. The catalyst film present
on the Si surface after MACE was removed before the reflectance measurements:
Pure Au films were etched in a KI/I2 solution (10 wt. %
KI, 5 wt. % I2) for 2 h, and Au/Ag films were etched first
in the same KI/I2 solution for 2 h and then in a 1:1 H2O:HNO3 solution for 2 h. A 5 mm circular pinhole
was used to select the area of interest. The baseline correction was
performed after aligning the light beam on the pinhole by using a
white Spectralon reference. The reflectance spectra were recorded
between 200 and 1200 nm with a 2 nm step.
spectroscopy was carried out using a PerkinElmer Lambda 1050 equipped
with a 150 mm integrating sphere and PbS and InGaAs detectors, collecting
both the diffuse and the specular reflectance. The catalyst film present
on the Si surface after MACE was removed before the reflectance measurements:
Pure Au films were etched in a KI/I2 solution (10 wt. %
KI, 5 wt. % I2) for 2 h, and Au/Ag films were etched first
in the same KI/I2 solution for 2 h and then in a 1:1 H2O:HNO3 solution for 2 h. A 5 mm circular pinhole
was used to select the area of interest. The baseline correction was
performed after aligning the light beam on the pinhole by using a
white Spectralon reference. The reflectance spectra were recorded
between 200 and 1200 nm with a 2 nm step.
9
Nanoparticle Composition Analysis by UV-Vis
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The composition of the nanoparticles was verified by UV-Vis spectrophotometry (Perkin Elmer Lambda 1050, USA). For this purpose, the UV-Vis spectra of pure res, pure Pluronic F127, pure Vitamin E TPGS, res-loaded-nanoparticles and empty nanoparticles were attained. The samples were scanned at a wavelength of 250-850 nm. All solids and lyophilized nanoparticles were dissolved in DMSO prior to analysis.
10
UV-Vis-IR Spectrophotometry Analysis
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UV-vis-infrared spectrophotometry (Perkin-Elmer Lambda 1050) was performed for both clear and pigment fluids.
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