Log In Sign up
The largest database of trusted experimental protocols

Raptinal

Manufactured by Merck Group
Visit Supplier

Raptinal is a laboratory equipment product developed by Merck Group. It is designed for the analysis and processing of biological samples. The core function of Raptinal is to facilitate the extraction, separation, and purification of target analytes from complex matrices. The device utilizes advanced chromatographic techniques to achieve high-performance separation and isolation of desired compounds.

Automatically generated - may contain errors

Lab products found in correlation

Z vad fmk, by BD (2 mentions) Z devd fmk, by BD (2 mentions) Celltiter 96 kit, by Promega (2 mentions) Vybrant did dye, by Thermo Fisher Scientific (2 mentions) Desferoxamine dfo, by Merck Group (2 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Sytox green, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) A5 fitc, by BD (1 mentions) A5 binding buffer, by BD (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Dextran sulfate sodium (dss), by Merck Group (1 mentions) Streptavidin peroxidase, by Merck Group (1 mentions) Bcl2 associated x (bax), by BioLegend (1 mentions) 1 2 dimethylhydrazine, by Merck Group (1 mentions) Biotinylated goat anti rabbit immunoglobulin g, by Merck Group (1 mentions)

7 protocols using raptinal

1

Apoptosis Induction Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Raptinal, anti-Fas (clone CH11), cytochalasin D (Cyto-D), carbenoxolone (CBX), trovafloxacin (trova), dimethyl sulfoxide (DMSO) and poly-l-lysine were purchased from Sigma-Aldrich. A5-FITC, A5-PE and A5 binding buffer were purchased from BD Bioscience. TO-PRO-3, RPMI 1640 medium, DMEM (1 g/L glucose) medium, Penicillin Streptomycin solution and Hoechst 33342 were purchased from Thermo-Fisher Scientific. Fetal calf serum (FCS) was purchased from Scientifix. ABT-737 and S63845 were purchased from Jomar Life Research.
Santavanond J.P., Chiu Y.H., Tixeira R., Liu Z., Yap J.K., Chen K.W., Li C.L., Lu Y.R., Roncero-Carol J., Hoijman E., Rutter S.F., Shi B., Ryan G.F., Hodge A.L., Caruso S., Baxter A.A., Ozkocak D.C., Johnson C., Day Z.I., Mayfosh A.J., Hulett M.D., Phan T.K., Atkin-Smith G.K, & Poon I.K. (2024). The small molecule raptinal can simultaneously induce apoptosis and inhibit PANX1 activity. Cell Death & Disease, 15(2), 123.
+ Open protocol
+ Expand
2

Evaluating Cell Death Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Raptinal, EGTA, necrostatin-1s (Nec-1s), ferrostatin-1 (Fer-1), α-tocopherol (Vit. E), and desferoxamine (DFO) were from Sigma. TRIzol was from Life Technologies. TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences. SYTOX green was from Invitrogen. Vybrant DiD dye was from ThermoFisher Scientific. CellTiter 96 kit (Promega) was used to measure cell proliferation.
Zhang Z., Zhang Y., Xia S., Kong Q., Li S., Liu X., Junqueira C., Meza-Sosa K.F., Temy Mok M.Y., Ansara J., Sengupta S., Yao Y., Wu H, & Lieberman J. (2020). Gasdermin E suppresses tumor growth by activating anti-tumor immunity. Nature, 579(7799), 415-420.
+ Open protocol
+ Expand
3

Decellularization of Extracellular Matrix

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
GL261 cells were plated and cultured for one week under crowded conditions with Ficoll 70/400, as described above. To isolate deposited matrix molecules for further experiments, we first decellularized the wells by treating with the pro-apoptotic agent raptinal [Palchaudhuri et al., 2015 (link)]. Wells for apoptosis were treated with 10 μm raptinal (Sigma SML1745) and incubated for 1.5–2 hours at 37°C until all the cells detach from the well-plate. The well-plates were then washed three times with PBS for 10 minutes each followed by a 2 day wash with PBS to ensure complete removal of the raptinal.
Louisthelmy R., Burke B.M, & Cornelison R.C. (2022). Brain cancer cell-derived matrices and effects on astrocyte migration. Cells, tissues, organs, 212(1), 21-31.
+ Open protocol
+ Expand
4

Evaluating Cell Death Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Raptinal, EGTA, necrostatin-1s (Nec-1s), ferrostatin-1 (Fer-1), α-tocopherol (Vit. E), and desferoxamine (DFO) were from Sigma. TRIzol was from Life Technologies. TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences. SYTOX green was from Invitrogen. Vybrant DiD dye was from ThermoFisher Scientific. CellTiter 96 kit (Promega) was used to measure cell proliferation.
Zhang Z., Zhang Y., Xia S., Kong Q., Li S., Liu X., Junqueira C., Meza-Sosa K.F., Temy Mok M.Y., Ansara J., Sengupta S., Yao Y., Wu H, & Lieberman J. (2020). Gasdermin E suppresses tumor growth by activating anti-tumor immunity. Nature, 579(7799), 415-420.
+ Open protocol
+ Expand
5

Colorectal Cancer Pathogenesis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Entire chemicals have been an analytical reagent brand. Raptinal (97% pure by HPLC), Dextran sulfate sodium (DSS), 1,2 dimethylhydrazine (DMH), streptavidin peroxidase, biotinylated goat anti-rabbit immunoglobulin G, proteinase K and 3,3'-diaminobenzidine (DAB) been obtained by Sigma Aldrich Chemical Co.(St. Louis, MO, USA). Rabbit anti-rat Bcl-2, p53, caspase-3, Bax, IL-6, TNF-α, and Ki-67 have been purchased through BioLegend (San Diego, California, USA). The apoptotic kit has been procured by Takara Bio Inc (Kusatsu, Japan).
Lan Y., Yang Y., Das A., Bhattacharya B, & Roy S. (2023). Raptinal ameliorates 1,2-dimethylhydrazine-induced colon cancer through p53/Bcl2/Bax/caspase-3-mediated apoptotic events in vitro and in vivo. Indian Journal of Pharmacology, 55(2), 97-107.
+ Open protocol
+ Expand
6

Assessing Toxin-Induced Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Raptinal (cat# SML1745), rotenone (cat# R8875), antimycin-A (cat# A8674) and 3-Nitropropionic acid (cat# N5636) were purchased from Sigma and resuspended in DMSO to make stock solutions. For Raptinal treatments, cells were exposed to final concentrations ranging from 1–10uM for 1h, and then incubated with fresh media and assessed at later timepoints. For antimycin-A treatments, cells were exposed to final concentrations ranging from 5–20 uM for 30 min and then incubated with fresh media. For rotenone and 3-NP treatments, cells were exposed to working concentrations ranging from 10–30uM (rotenone) and 1–3 mM (3-NP) for 2h and then incubated with fresh media. Z-VAD-FMK (R&D cat# FMK001) was resuspended in DMSO and used at a final concentration of 20 uM. For experiments using toxins and z-VAD, neurons were pretreated with 20uM z-VAD for 30 min, and then treated with a combination of zVAD (20uM) and toxins for the indicated timepoints.
Tan S.L., Nakao H., He Y., Vijaysri S., Neddermann P., Jacobs B.L., Mayer B.J, & Katze M.G. (1999). NS5A, a nonstructural protein of hepatitis C virus, binds growth factor receptor-bound protein 2 adaptor protein in a Src homology 3 domain/ligand-dependent manner and perturbs mitogenic signaling. Proceedings of the National Academy of Sciences of the United States of America, 96(10), 5533-5538.
+ Open protocol
+ Expand
7

Caspase 3/7 Assay in Thyrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thyrospheres and differentiated thyrocytes cultured in the presence or the absence of Na 2 WO 4 for 15 days were exposed for 4 h to the apoptosis-inducing agents staurosporine (1 μM, Sigma) or raptinal (10 μM, Sigma). Caspase 3/7 activity was measured using the Caspase-Glo 3/7 assay (Promega) according to the manufacturer's protocol.
Gianì F., Pandini G., Scalisi N.M., Vigneri P., Fazzari C., Malandrino P., Russo M., Masucci R., Belfiore A., Pellegriti G, & Vigneri R. (2019). Effect of low-dose tungsten on human thyroid stem/precursor cells and their progeny. Endocrine-related cancer, 26(8).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!