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Mouse ferritin elisa kit

Manufactured by ALPCO
Sourced in United States

The Mouse Ferritin ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of ferritin levels in mouse serum, plasma, or cell culture supernatants. The kit utilizes a specific antibody coated on a microplate to capture ferritin, which is then detected by a horseradish peroxidase (HRP) conjugated antibody and a colorimetric substrate. The absorbance is measured, and the ferritin concentration is determined using a standard curve.

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2 protocols using mouse ferritin elisa kit

1

Cytokine and Ferritin ELISA Assays

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Mouse tumor necrosis factor–α (TNF-α), IL-12/23, IL-12, IL-6, and IFN-γ enzyme-linked immunosorbent assay (ELISA) kits were purchased from BioLegend, USA. Mouse Ferritin ELISA kit was procured from ALPCO Diagnostics, USA. All kits were used according to the instructions of manufacturers, as detailed in our previous work (15 (link)).
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2

Measuring Iron and Inflammation Biomarkers

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Serum iron and transferrin saturation were measured using the Iron/TIBC reagent set (Pointe Scientific, US) according to the manufacturer’s instructions. Serum ALT and AST were determined using assay kits (Shensuoyoufu, China). Serum ferritin was quantitatively determined using mouse ferritin ELISA kit (ALPCO Diagnostics, US) and serum IL-6 was detected using mouse IL-6 ELISA kit (R&D systems, US). Measurement of tissue non-heme iron was performed using a ferrozine-based method described before14 (link).
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