Hy 112879

Manufactured by MedChemExpress

HY-112879 is a laboratory product offered by MedChemExpress. It is a piece of equipment used for scientific research and analysis purposes. The core function of this product is to facilitate various experimental and testing procedures in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Mito tempo, by MedChemExpress (1 mentions) Hy 15886, by MedChemExpress (1 mentions) Hy 10231, by MedChemExpress (1 mentions) Hy 12815, by MedChemExpress (1 mentions)

2 protocols using hy 112879

Liver Ischemia-Reperfusion Injury Model

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

As mentioned previously,⁷ (link)¹⁷ (link) a stable mouse model was established using 70% partial hepatic heat IR. Mice were anesthetized and a laparotomy was performed to expose the liver. The left and middle branches of the intrahepatic portal vein were clamped to block the blood supply. The clips were released for reperfusion after 90 minutes of ischemia. The sham mice underwent the same procedure but without clamping the blood vessels. The mice were sacrificed 6 hours after reperfusion, and liver and blood samples were collected for analysis.
To study the effects of ER stress, Control and HFD-fed mice were injected with tauroursodeoxycholic acid (TUDCA) i.p. (400 mg/kg) or PBS (Control) for 3 days, and then the model of liver IR was established.
To determine the role of mitochondrial oxidation, Control and HFD-fed mice were pretreated with the mitochondria-targeted antioxidant Mito-TEMPO (5 mg/kg, MedChemExpress, HY-112879) twice at 17 hours and 1 hour before surgery or PBS.

Li F., Guan Z., Gao Y., Bai Y., Zhan X., Ji X., Xu J., Zhou H, & Rao Z. (2024). ER stress promotes mitochondrial calcium overload and activates the ROS/NLRP3 axis to mediate fatty liver ischemic injury. Hepatology Communications, 8(4), e0399.

+ Open protocol

+ Expand

Pharmacological Modulation of HIF-1α, NLRP3, Drp1, and ROS in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

For HIF‐1α inhibition, mice were administered PX‐478 (5 mg/kg, HY‐10231, MedChemExpress) in phosphate‐buffered saline (PBS) solution by oral gavage every other day, and the control mice received an equivalent amount of PBS. For NLRP3 inhibition, mice were gavaged with MCC950 (20 mg/kg, HY‐12815, MedChemExpress) or vehicle (.9% NaCl) every day for 5 days/week. For Drp1 inhibition, mice were given mitochondrial division inhibitor 1 (Mdivi‐1) (20 mg/kg, dissolved in dimethyl sulfoxide (DMSO), HY‐15886, MedChemExpress) intraperitoneally, and the control mice were injected with the same volume of DMSO. For ROS scavenging, mice were intraperitoneally injected with mito‐TEMPO (MT, dissolved in PBS, 10 mg/kg, HY‐112879, MedChemExpress) every other day, and the mice in the control group were given an equal volume of PBS intraperitoneally.

Xiao Y., Liu X., Xie K., Luo J., Zhang Y., Huang X., Luo J, & Tan S. (2024). Mitochondrial dysfunction induced by HIF‐1α under hypoxia contributes to the development of gastric mucosal lesions. Clinical and Translational Medicine, 14(4), e1653.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!