Sodium alginate (MW = 250000 Da) and carboxymethyl cellulose (MW = 90.000 g/mol) were purchased from Sigma Aldrich as sources of natural polymers, FeCl3‧6H2O, FeCl3‧6H2O and FeCl2·4H2O (Merck Co.) were used as precursors of Fe3O4 NPs, CuSO4·5H2O (Merck Co.) was used as a precursor of Cu NPs, and 4-nitrophenol (Merck Co.), sodium borohydride (NaBH4), and sodium citrate (Merck Co.) were used. All of the materials were used as received without further purification.
Fecl3 6h2o
Manufactured by Merck Group
Sourced in United States, Germany, Spain, India, China, Switzerland, Singapore, United Kingdom, Denmark, France, Italy
FeCl3·6H2O is a chemical compound that consists of ferric chloride (FeCl3) crystalized with six water molecules (6H2O). It is a common inorganic compound used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Fecl2 4h2o, by Merck Group (74 mentions)
Sodium hydroxide (naoh), by Merck Group (33 mentions)
Feso4 7h2o, by Merck Group (28 mentions)
Hydrochloric acid (hcl), by Merck Group (27 mentions)
Ethanol, by Merck Group (18 mentions)
Gallic acid, by Merck Group (15 mentions)
Methanol, by Merck Group (15 mentions)
Sodium chloride (nacl), by Merck Group (14 mentions)
Cocl2 6h2o, by Merck Group (13 mentions)
Acetic acid, by Merck Group (12 mentions)
Trolox, by Merck Group (11 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (11 mentions)
Nh4oh, by Merck Group (11 mentions)
Cucl2 2h2o, by Merck Group (10 mentions)
Quercetin, by Merck Group (10 mentions)
Nicl2 6h2o, by Merck Group (10 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (10 mentions)
Oleic acid, by Merck Group (9 mentions)
Ascorbic acid, by Merck Group (9 mentions)
Mncl2 4h2o, by Merck Group (9 mentions)
285 protocols using fecl3 6h2o
1
Synthesis of Metal Nanoparticles
2
Nanoparticle Synthesis: Protocols and Materials
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FeCl2·4H2O, FeCl3·6H2O and NOR used in the nanoparticle synthesis were purchased from Sigma-Aldrich, Germany. 25% ammonia solution was purchased from Merck, Germany (GR grade).
RPMI-1640 and fetal bovine serum (FBS) used for the culturing of THP1 cells were purchased from HiMedia, India. Phorbol 12 myristate acetate (PMA) used for THP1 cell differentiation was purchased from Sigma-Aldrich, Germany.
RPMI-1640 and fetal bovine serum (FBS) used for the culturing of THP1 cells were purchased from HiMedia, India. Phorbol 12 myristate acetate (PMA) used for THP1 cell differentiation was purchased from Sigma-Aldrich, Germany.
3
Immobilization of Lipases on Magnetic Silica Nanoparticles
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Lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML), Candida antarctica lipase B (CALB), FeCl 2 .4H 2 O, FeCl 3 .6H 2 O, p-nitrophenyl butyrate (p-NPB), and tetraethyl orthosilicate (TEOS) were supplied from Sigma-Aldrich. Waste cooking oil was also obtained from a local restaurant and the fatty acids composition of waste cooking oil was 40.6% oleic acid, 17.58% linoleic acid, 32.24% palmitic acid, 5.23% stearic acid. The molecular weight of the waste cooking oil which was determined from the saponi cation value of 196.2 mg KOH/g and acid value of 76 mg KOH/g was calculated to be 856.3 g/mol. Water content in the oil measured by Karl Fischer (Verhoef et al. 1978 (link)) titration method was determined to be 0.01% (w/w).
Solvents used including n-hexane, methanol, ethanol, 1-propanol, toluene, t-butanol and blue silica gel were prepared from Merck chemicals. Triethylamine (Et 3 N) was from Titrachem and 3-aminopropyltrimethoxysilane (3-APTMS) 97% was purchased from Alfa Aesar. All the other chemicals were accessed commercially. ethyl alcohol for several times to reach the pH value of 7.0. Then, the magnetic nanoparticles were dried in a vacuum oven at 80°C and used directly for being coated with silica.
Solvents used including n-hexane, methanol, ethanol, 1-propanol, toluene, t-butanol and blue silica gel were prepared from Merck chemicals. Triethylamine (Et 3 N) was from Titrachem and 3-aminopropyltrimethoxysilane (3-APTMS) 97% was purchased from Alfa Aesar. All the other chemicals were accessed commercially. ethyl alcohol for several times to reach the pH value of 7.0. Then, the magnetic nanoparticles were dried in a vacuum oven at 80°C and used directly for being coated with silica.
4
Biochemical Assays for Cell Cytotoxicity
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3-ethyl benzothiazoline-6-sulfonic acid (ABTS) and 3-mercaptopropanoic acid (HSCH2CH2CO2H) were brought from Sigma–Aldrich (St. Louis, MO, USA). Other chemicals of analytical grade, FeCl3·6H2O, FeSO4·7H2O and 1-hydroxybenzotriazole (HBT) were obtained from Sigma Chemical Co., Cairo, Egypt. Methyl Orange (MO), Reactive Black-5 (RB-5), Brilliant blue (Bb), and Remazol Brilliant Blue R (RBBR) were purchased from Sigma–Aldrich. All other reagents and chemicals used in the present research were of analytical grade. MCF-7 (human breast cancer cells), HepG2 (human hepatocellular carcinoma cells), and A549 cell lines were brought from ATCC via the holding company for biological products and vaccines (VACSERA), Cairo, Egypt.
5
Antioxidant Evaluation of Natural Compounds
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Caffeic acid (98%, CAF) was purchased from Riedel de Haën (Seelze, Germany). Gallic acid (99.5%, GAL), DPPH radical (1,1-diphenyl-2-picrylhydrazyl, 90%), 2,4,6-tripyridyl-s-triazine (TPTZ), FeCl3·6H2O, and quercetin (QUERC) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Folin–Ciocalteu reagent, AlCl3, and Νa2CO3 (99.8%) were from Panreac Quimica (Barcelona, Spain). HPLC-grade methanol (MeOH) and ethanol (EtOH), as well as acetic acid were from Merck (Darmstadt, Germany). All other common reagents and solvents were of the appropriate purity from various suppliers. The water used was deionized, obtained by an ion-exchange resin system (ZALION 2000, IONEL, Athens, Greece) with a minimum resistance of 800,000 Ω/cm.
6
Synthesizing Magnetic Nanoparticles via Colloidal Methods
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Oleylamine (OLA, 70%), potassium chloride KCl (99%), the manganese precursor MnCl2 (99%) and the iron precursor FeCl3 × 6H2O (98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tri-octylphospine (TOP, 97%) was purchased from Strem chemicals (Newburyport, MA, USA). Organic solvents like acetone and hexane were of analytical grade and obtained from various sources. All chemicals were used as received without any further purification. Some of the experiments were carried out using standard airless techniques: a vacuum/dry nitrogen gas Schlenk line was used for synthesis and an Ar glovebox for storing and handling air- and moisture-sensitive chemicals.
7
Preparation of Fe-Siderophore Solutions
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To study the suitability of each bacterial media for Fe chlorosis remediation in soil applications, an Fe-siderophore solution (ISS) was prepared by rehydrating to the same volume the previously obtained powders; this procedure allowed to achieve the same initial concentration of siderophores. Then, a soluble salt of Fe(III), FeCl3.6H2O (Sigma), was added to the maximum complexation capacity at pH 9.0 (A. vinelandii ≈ 188 µmol L−1 and B. subtilis ≈ 225 µmol L−1) of each solution, which was previously determined (Ferreira et al., 2019b (link)). During the process, the pH of the solution was kept between 7.0 and 9.0, and the final pH was set at 9.0. The solution was allowed to settle overnight and filtered (0.45-µm pore size filter). The initial Fe concentration was then measured by atomic absorption spectroscopy with flame atomization (AAS-FA) using a Perkin-Elmer AAnalyst 400 spectrometer (Norwalk, CT, USA).
8
Sulfuric Acid-Metal Ion Co-Catalyst Pretreatment
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The main chemicals used were 96% sulfuric acid (catalog no. 9681–03; J. T. Baker Chemical Co., Phillipsburg, NJ, USA), FeCl2·4H2O (catalog no. 44939; Sigma-Aldrich, St. Louis, MO, USA), and FeCl3·6H2O (catalog no. 236480; Sigma-Aldrich, St. Louis, MO, USA). For DA/metal ion co-catalyst pretreatment, the 0.5 wt% H2SO4 was prepared beforehand. One hundred milliliters of acid solution was distributed into a 250-mL serum bottle, and the solvent was pre-sparged for 5 min with either air to make it oxygen-saturated (for aerobic setup condition), or N2 gas (for anaerobic setup condition) using a long needle that reached to the bottom of the bottle. Then, an appropriate amount of FeCl2 or FeCl3 was added into the bottle to reach the designated concentrations. Following that, the dilute sulfuric acid/metal ion co-catalyst solution was directly capped (for aerobic setup condition), or re-sparged with N2 gas for 5 min (for anaerobic setup condition), and then the bottles were capped with Teflon stoppers and aluminum crimps (Figure 4 , step 1).
9
Vibrio parahaemolyticus Aptamer Synthesis
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FeCl2·4H2O, FeCl3·6H2O, HAuCl4, KCl, MgCl2·6H2O, NaCl, and ethanol were
purchased from Sigma-Aldrich (Singapore). NaH2PO4·2H2O was obtained from Ajax Finechem (New Zealand).
Thiosulfate-citrate-bile-salts-sucrose agar (TCBS Agar) was purchased
from Difco (France). Trisodium citrate dihydrate and MgCl2·6H2O were obtained from Merck (Germany). Ammonia
solution (28% w/v) and Na2HPO4 were purchased
from QREC (New Zealand). N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich.
The DNA aptamer having a sequence specific to V. parahaemolyticus was selected and identified by whole-bacterium SELEX, which was
designed and reported by previously published research work.28 (link),31 (link),33 (link),34 (link) The sequence of the V. parahaemolyticus aptamer is 5′-NH2-TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT-3′
(NH2-aptamer) and 5′-SH-TCTAAAAATGGGCAAAAAACAGTGACTCGTTGAGATACT-3′
(SH-aptamer). The aptamers were synthesized by the Integrated and
Technologies Company (Bangkok, Thailand). The binding buffer was prepared
with 50 mM Tris–HCl, 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2 at pH 7.4. Phosphate buffer solution was prepared with 0.1
M Na2HPO4 and 0.1 M NaH2PO4·2H2O.
purchased from Sigma-Aldrich (Singapore). NaH2PO4·2H2O was obtained from Ajax Finechem (New Zealand).
Thiosulfate-citrate-bile-salts-sucrose agar (TCBS Agar) was purchased
from Difco (France). Trisodium citrate dihydrate and MgCl2·6H2O were obtained from Merck (Germany). Ammonia
solution (28% w/v) and Na2HPO4 were purchased
from QREC (New Zealand). N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich.
The DNA aptamer having a sequence specific to V. parahaemolyticus was selected and identified by whole-bacterium SELEX, which was
designed and reported by previously published research work.28 (link),31 (link),33 (link),34 (link) The sequence of the V. parahaemolyticus aptamer is 5′-NH2-TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT-3′
(NH2-aptamer) and 5′-SH-TCTAAAAATGGGCAAAAAACAGTGACTCGTTGAGATACT-3′
(SH-aptamer). The aptamers were synthesized by the Integrated and
Technologies Company (Bangkok, Thailand). The binding buffer was prepared
with 50 mM Tris–HCl, 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2 at pH 7.4. Phosphate buffer solution was prepared with 0.1
M Na2HPO4 and 0.1 M NaH2PO4·2H2O.
10
Synthesis and Evaluation of Tocopherol Derivatives
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FeCl2·4 H2O, FeCl3·6 H2O, N,N-dimethylacrylamide (DMA), 4,4′-azobis(4-cyanovaleric acid) (ACVA), NaCl, Tris, thiobarbituric acid (TBA), trichloroacetic acid, copper iron oxide (CuFe2O4), 1,10-phenanthroline, Toc, and cell culture medium 199 were obtained from Sigma-Aldrich (St Louis, MO, USA). The Toc derivative with an isoprenoid side chain shortened to 6 carbon atoms (Figure 1 ) was synthesized both in its phenolic (Toc-6-OH) and acetate forms (Toc-6-Ac) according to a method described previously.21 Doxorubicin was obtained from Arterium (Kiev, Ukraine), and a sodium hypochlorite solution was obtained from Bochemie (Bohumín, Czech Republic). Other reagents and solvents were purchased from LachNer (Neratovice, Czech Republic). Ultrapure Q water, ultrafiltered in a Milli-Q Gradient A10 system (Millipore; Molsheim, France), was used to prepare solutions.
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