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Monosialoganglioside gm2

Manufactured by Matreya
Sourced in United States

Monosialoganglioside GM2 is a glycosphingolipid compound found in cell membranes. It serves as a structural component and plays a role in cell signaling processes. The core function of Monosialoganglioside GM2 is to participate in cellular interactions and communication.

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2 protocols using monosialoganglioside gm2

1

Neuronal Culture and Characterization

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Dulbecco’s Modified Eagle’s Medium (DMEM) (#11995065), Neurobasal Medium (#21103049), trypsin-EDTA 0.25% (#25200056), penicillin-streptomycin (#15140122), B27 Supplement (#17504944), and Glutamax (#35050061) were from Life Technologies (Carlsbad, CA, USA), Fetal calf serum (FCS) was from Internegocios SA (Mercedes, Argentina). The protease inhibitors, purchased from Santa Cruz Biotechnology (Dallas, TX, USA), were leupeptin hemisulfate (#295358), pepstatin A (#45036), aprotinin (#3595), and phenylmethylsulfonyl fluoride (#329-98-6). Monosialoganglioside GM2 (#1502) was obtained from Matreya (State College, PA, USA). The primary antibodies used were anti-P-PERK (#3179), anti-CHOP (#L63F7) (Cell Signaling Technology, Danvers, MA, USA), anti-CHOP (#MA1-250) (Thermo Fisher, Waltham, MA, USA), anti-MAP-2 (#PCK-554P) (Covance Inc. Princeton, NJ, USA), and anti-MAP-2 (#M2320) (Sigma, St. Louis, MO, USA).
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2

Reconstitution of Cholera Toxin Receptors

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Cholera toxin (CT) from Vibrio cholera, Triton X-100, 1-octadecanethiol (98%), and n-octadecyltrichlorosilane (OTS, 90+%) were from Sigma-Aldrich (St. Louis, MO). Trehalose was from Swanson Health Products (Fargo, ND).
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-|6-[(7-nitro-2−1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) were from Avanti Polar Lipids (Alabaster, AL). Monosialoganglioside GM1 (NH4+ salt) and monosialoganglioside GM2 (NH4+ salt) were from Matreya (Pleasant Gap, PA). Monosialoganglioside GM3 was from EMD Biosciences (La Jolla, CA). BK-7 glass substrates were from Corning (Painted Post, NY). Chromium and gold used for electron-beam evaporation were acquired as pellets of 99.99% purity from Kurt J. Lesker (Jefferson Hills, PA).
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