A small layer of gold was applied to the film specimens before they were placed on aluminum stubs with double-sided tape. A scanning electron microscope (Jeol, model JSM-5800, Tokyo, Japan) operating at 5 kV and a magnification of 1000 kx was used for morphological observations on the surface of the films (which were broken under liquid nitrogen before imaging).
Jsm 5800
Manufactured by JEOL
Sourced in Japan
The JSM-5800 is a Scanning Electron Microscope (SEM) manufactured by JEOL. It is designed to provide high-resolution imaging of a wide range of materials. The core function of the JSM-5800 is to generate and focus an electron beam, which is then scanned across the surface of a sample, generating signals that are used to create an image.
Automatically generated - may contain errors
Lab products found in correlation
Pw2404, by Philips (2 mentions)
Digital caliper, by Mitutoyo (1 mentions)
Celltracker green cmfda, by Thermo Fisher Scientific (1 mentions)
Celltracker orange cmtmr, by Thermo Fisher Scientific (1 mentions)
Jem 2100, by JEOL (1 mentions)
D max 2200 pc, by Rigaku (1 mentions)
Nicolet in10 mx, by Thermo Fisher Scientific (1 mentions)
D max rc x ray diffractometer, by Rigaku (1 mentions)
Microscope objective, by Olympus (1 mentions)
Ix73 inverted microscope, by Olympus (1 mentions)
Ir spectroscopy grade, by Merck Group (1 mentions)
Jsm 7500fam, by JEOL (1 mentions)
Energy dispersive spectrometer, by JEOL (1 mentions)
Malvern laser particle size analyzer zs90, by Malvern Panalytical (1 mentions)
Nicolet 6700 ft ir instrument, by Thermo Fisher Scientific (1 mentions)
X ray diffractometer xrd, by Malvern Panalytical (1 mentions)
Lake shore 7400, by Lake Shore Cryotronics (1 mentions)
Eclipse lv150n, by Nikon (1 mentions)
Dma q800, by TA Instruments (1 mentions)
D max 2200, by Rigaku (1 mentions)
35 protocols using jsm 5800
1
Surface Morphology of Film Specimens
2
Characterizing Polyurethane Foam Microstructure
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The fabricated foam’s
microstructure was examined by electron
microscopy, by Philips-type JEOL JSM 5800 functioning at 10 kV was
put to use. A 1 mm breadth was cut off the foam samples in the x-direction.
The pore measurement of the foams was identified from the images obtained
from electron microscopy through the open-source ImageJ software.
We have obtained good pictures that show how the loads are spread
out on the surfaces of the polyurethane foam cells. ImageJ software
evaluated the morphological characteristics of the polyurethane foam.
microstructure was examined by electron
microscopy, by Philips-type JEOL JSM 5800 functioning at 10 kV was
put to use. A 1 mm breadth was cut off the foam samples in the x-direction.
The pore measurement of the foams was identified from the images obtained
from electron microscopy through the open-source ImageJ software.
We have obtained good pictures that show how the loads are spread
out on the surfaces of the polyurethane foam cells. ImageJ software
evaluated the morphological characteristics of the polyurethane foam.
3
Scanning Electron Microscopy of Gold-Coated Samples
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To examine the surface morphology, scanning electron microscopic (SEM) studies were performed on gold-coated samples using a scanning electron microscope JSM 5800 (JEOL, Tokyo, Japan) at 10 kV at a magnification of 5k.
4
Cyclic Fatigue Testing of Heat-Treated Endodontic Files
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A non-tapered custom-made stainless-steel tube model apparatus was used for the
cyclic fatigue test as reported in previous studies 10 (link),12 (link),14 (link). Ten instruments of each type (original Profile,
yellowish Profile - heat-treat at 450° C, and blueish Profile - heat-treated at
500° C) were activated in a continuous clockwise rotation by a 6:1 reduction
handpiece (Sirona Dental Systems GmbH, Bensheim, Germany) powered by a
torque-controlled motor (VDW Silver; VDW GmbH, Munich, Germany) at a static
position using glycerin as lubricant. The time to fracture (in seconds) was
established when the fracture was detected by visual and auditory inspection,
and the size (in mm) of the fractured segments recorded using a digital caliper
(Mitutoyo) for experimental control.
After tests, a scanning electron microscope (SEM) (JSM 5800; JEOL, Tokyo, Japan)
was used to analyze the fracture surfaces of all the tested instruments in order
to observe the fracture mode. Different magnifications were used (× 250, and ×
1500).
cyclic fatigue test as reported in previous studies 10 (link),12 (link),14 (link). Ten instruments of each type (original Profile,
yellowish Profile - heat-treat at 450° C, and blueish Profile - heat-treated at
500° C) were activated in a continuous clockwise rotation by a 6:1 reduction
handpiece (Sirona Dental Systems GmbH, Bensheim, Germany) powered by a
torque-controlled motor (VDW Silver; VDW GmbH, Munich, Germany) at a static
position using glycerin as lubricant. The time to fracture (in seconds) was
established when the fracture was detected by visual and auditory inspection,
and the size (in mm) of the fractured segments recorded using a digital caliper
(Mitutoyo) for experimental control.
After tests, a scanning electron microscope (SEM) (JSM 5800; JEOL, Tokyo, Japan)
was used to analyze the fracture surfaces of all the tested instruments in order
to observe the fracture mode. Different magnifications were used (× 250, and ×
1500).
5
Osteoblast-Macrophage Co-Culture Imaging
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For direct co-culture the osteoblast (0.5 × 105/cm2) and macrophage (105 cells/cm2) cells were pre-stained with CellTracker™ Green CMFDA (Invitrogen) and CellTracker™ Orange CMTMR (Invitrogen) respectively for 30 min following the Manufacturer's protocol. Cell seeded surfaces were treated as per standard protocol and fixed with 2% PFA. Imaging was carried out using 488 and 543 nm laser in CLSM (FV1000, Olympous, Japan) in sequential mode to analyze the cell adhesion efficiency.
Adhered cell morphology in co-culture model was analyzed by fixing the surface with 2% PFA and prepared for SEM analysis by gradual dehydration. The samples were then gold sputtered and analyzed using SEM (JEOL JSM 5800).
Adhered cell morphology in co-culture model was analyzed by fixing the surface with 2% PFA and prepared for SEM analysis by gradual dehydration. The samples were then gold sputtered and analyzed using SEM (JEOL JSM 5800).
6
Atmospheric Mineral Dust Characterization
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ASD was collected from the atmosphere on March 16, 2009 outside the Gachon University building in Korea, using a high volume air sampler (HV500F; Sibata Scientific Technology, Ltd., Saitama, Japan) at a flow rate of 500 L/min. These ASD samples were prefiltered into filter packs (Prefilter AP, 124 mm; EMD Millipore, Bedford, MA, USA) and sieved through a filter with a 10-μm pore size, after mixing with phosphate buffered saline (PBS) in a 15-mL tube. The filtered ASD particles were sterilized in an autoclave at 121°C for 15 minutes; subsequently, these particles were weighed. Each sample was transferred to a 1.5-mL tube. The filtered PM was stored at –20°C until further use. The particle diameter of the samples was measured (a total of 600 particles) under a scanning electron microscope (JSM-5800; JEOL Ltd., Tokyo, Japan). The mean distribution peak of the ASD particle diameter was observed at 6 μm. Chemical components of the ASD particles were analyzed via X-ray fluorescence spectrometry (PHILIPS pw2404; Philips, Eindhoven, The Netherlands) at the Korea Basic Science Institute. The chemical composition of ASD was determined to be as follows: 78.4% SiO2, 9.35% Al2O3, 2.52% K2O, 2.41% Na2O, 2.06% Fe2O3, and 1.74% CaO. MaO, TiO2, P2O5, and MnO made up less than 0.1% of the total composition.
7
Visualization and Characterization of Lignin-Based Polymers
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HR-TEM was performed in JEOL JEM 2100 instrument. One mg/mL concentration of lignin and lig-b-POZ copolymer in aqueous solution was drop cast on a Cu-grid. After drying the grid at ambient temperature, the samples were directly imaged under TEM. To record the AFM images of lignin and Lig-g-POZ, 10 μL of 1 mg/mL concentrated samples in aqueous solution was directly spotted on the glass cover slip as a drop-caste method. Surface morphology was studied by using AFM and scanning electron microscope JEOL JSM 5800) with an accelerated voltage between 5–20 kV. Moreover, the bio-flim images were also carried out in same instruments. The dynamic light scattering experiment was performed with an argon ion laser system (DLS, Malvern Instruments, Series 4700) at 25 °C. One mg/mL concentration of aqueous solution of lignin and lig-g-POZ copolymer was used for analysis at pH 7 in aqueous media. DLS measurements of average particle size were performed with scattering angle of 90°. The standard deviation was calculated from more than twenty DLS measurements over a time period sufficient to reach equilibrium.
8
Mineral Particle Analysis using SEM-EDS
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Individual ochre particles were investigated using a field-emission scanning electron microscope (JEOL JSM-5800), fitted with an energy-dispersive X-ray analysis (EDS) to distinguish different mineral particles and their shape, size and elemental composition.
9
Pollen Preparation for SEM Imaging
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Using a dissecting scope, anthers from WT and mutant open flowers were removed with dissecting forceps (Sigma-Aldrich T4537). Pollen was released onto specimen stubs topped with double-sided sticky carbon tabs by gently tapping the forceps, or lightly tapping the anthers onto the stubs. The irx8 mutant anthers were first manually dissected to open them and pollen was gently scooped out using the forceps tip and transferred onto the stub surface. Samples were dehydrated and coated with gold particles for 120 s in a Sputter Coater, and imaged using either a JEOL JSM-5800 (SEM/EDAX) scanning electron microscope or a Topcon Aquila—Hybrid SEM.
10
Characterization of P(VDF-TrFE) Nanofibers
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The morphologies of the P(VDF-TrFE) nanofibers were examined by scanning electron microscopy (SEM, JSM-5800, JEOL, Akishima, Japan) at an accelerator voltage of 15 kV. Fast Fourier transforms (FFT) was performed on SEM images using the ImageJ software (National Institutes of Health, Bethesda, MD, USA) to determine the degrees of the nanofibers alignment. In order to analyze the compositions and crystallinities of the P(VDF-TrFE) nanofibers, Fourier transform infrared spectroscopy (FTIR, Nicolet iN10 MX, Thermo Fisher Scientific Inc., Waltham, MA, USA) in absorbance mode, and X-ray diffraction (XRD, D/MAX2200PC, Rigaku Corporation, Tokyo, Japan) over the 15–25° 2θ range were employed. A P(VDF-TrFE) spin-coated film was fabricated to study its diversity compared with the electrospun nanofibers. In addition, the P(VDF-TrFE) nanofibers were annealed at 130 and 140 °C for 2 h to investigate the variations in β-phase crystallinity.
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