Taz sirna

siRNA transfection was performed with siRNA reagent system (Santa Cruz, sc-45064) following the manufacturer’s instructions. Briefly, cells were replated in six-well plates to 40% confluence in antibiotic-free 10% FBS-containing medium. For each transfection, 6 μl of WT1 siRNA (Santa Cruz, sc-36846), TAZ siRNA (Thermo Fisher, 122501), and unconjugated control siRNA-A (Santa Cruz, sc-37007) in 100 μl of transfection medium (Santa Cruz, sc-36868) were mixed with 6 μl of transfection reagent (sc-29528) in another 100 μl of transfection medium. The mixtures were incubated for 30 min at room temperature. Each well was washed with 2 ml of transfection medium once and then filled with a prepared reagent mixture plus 0.8 ml of transfection medium. After incubation overnight, an additional 1 ml of medium supplemented with 20% FBS with 2% antibiotics was added to each well for another 24 h. The transfection mixture was removed and replaced with normal growth medium on the following day. All experimental measurements were performed 24 h following replacement of the medium. The sequence information of siRNAs will be available upon request.