The tissue and tumor samples were homogenized with a Bullet Blender (Next Advance, Averill Park, NY) according to the manufacturer’s instructions. Sequential extractions of frozen samples of tumors were performed using the CNMCS compartmental protein extraction kit (Millipore, Billerica, MA) as previously described [13 (link)]. In brief, frozen samples were homogenized and extracted sequentially to remove preferentially (1) cytosolic proteins, (2) nuclear proteins, (3) membrane proteins, (4) cytoskeletal proteins leaving a final insoluble fraction enriched for ECM proteins, although different tissues behave somewhat differently in the ease of extraction so that proteins sometimes appear in more than one fraction. The effectiveness of extraction of specific proteins was monitored by immunoblotting using the following antibodies: rabbit anti-collagen I, mouse anti-GAPDH and rabbit anti-histones (Millipore, Billerica, MA), the rabbit anti-actin antibody (serum 14–1) was generated in our laboratory.
Rabbit anti histones
Manufactured by Merck Group
Rabbit anti-histones is a laboratory reagent that can be used to detect and quantify histone proteins in biological samples. Histones are a group of proteins that play a crucial role in the packaging and organization of DNA within the cell nucleus. This product provides a specific antibody raised in rabbits that can recognize and bind to histone proteins, allowing for their identification and measurement in various experimental contexts.
Automatically generated - may contain errors
2 protocols using rabbit anti histones
1
Protein Extraction from Tissue Samples
2
ECM Enrichment of Islet Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Normal, hyperplastic and angiogenic islets and insulinomas were homogenized with a Bullet Blender (Next Advance, Averill Park, NY) according to the manufacturer’s instructions. Decellularization (removal of intracellular proteins) and concomitant enrichment of ECM proteins were performed using the CNMCS compartmental protein extraction kit (Millipore, Billerica, MA) as previously described8 (link)32 . The effectiveness of the ECM enrichment was monitored by immunoblotting using the following primary antibodies: rabbit anti-collagen I, mouse anti-GAPDH and rabbit anti-histones (Millipore, Billerica, MA), the rabbit anti-actin antibody (serum 14–1) was generated in our laboratory.
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