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Anti mcm2

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Anti-MCM2 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the MCM2 protein, which is a component of the mini-chromosome maintenance (MCM) complex involved in DNA replication. The primary function of Anti-MCM2 is to detect and quantify the presence of MCM2 in biological samples.

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Lab products found in correlation

Anti β actin, by Merck Group (3 mentions) Anti chk1, by Cell Signaling Technology (2 mentions) Anti cdk1, by Santa Cruz Biotechnology (2 mentions) P chk1, by Cell Signaling Technology (2 mentions) Anti pcdk1, by Cell Signaling Technology (2 mentions) Anti gh2ax, by Cell Signaling Technology (2 mentions) Anti parp1, by Cell Signaling Technology (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Colchicine, by Merck Group (2 mentions) Anti neun, by Merck Group (2 mentions) Nocodazole, by Merck Group (2 mentions) Anti sox2, by Merck Group (2 mentions) Anti ki67, by Agilent Technologies (2 mentions) Nimodipine, by Merck Group (2 mentions) Anti s100b, by Merck Group (2 mentions) Anti gfap, by Agilent Technologies (2 mentions) Anti mecp2, by Abcam (2 mentions) Anti map2, by Abcam (2 mentions) Anti mecp2, by Cell Signaling Technology (2 mentions) Anti nestin, by Aves Labs (2 mentions)

Close spelling variants of this product

Anti-Mcm2 polyclonal antibody N-19 (5 citations) Anti-MCM2 (N-19, (3 citations) Anti-MCM2 (sc-9839), (3 citations) Anti-MCM2 antibody (2 citations) Goat anti-MCM2 (2 citations)

9 protocols using anti mcm2

1

Comprehensive Immunoblotting Methodology

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Immunoblotting was performed as described previously (35 (link)). The following antibodies were used at a concentration of 0.1 to 0.5 μg/ml: anti-pMCM2 (3378-1; Epitomics Inc.), anti-MCM2 (sc-9839; Santa Cruz Biotechnology), anti–pPOL II (04-1571; Millipore), anti–POL II (05-952; Millipore), anti-Lamin B1 (ab16048; Abcam), anti-FANCD2 (sc-20022; Santa Cruz Biotechnology), anti-pCDC6 (ab76422; Abcam), anti-CDC6 (ab109315; Abcam), pChk1 (#2348; Cell Signaling Technology), anti-Chk1 (#2345; Cell Signaling Technology), anti-pCDK1 (#9111; Cell Signaling Technology), anti-CDK1 (sc-954; Santa Cruz Biotechnology), anti-gH2AX (#2577; Cell Signaling Technology), anti-PARP1 (#9542; Cell Signaling Technology), anti–cyclin B1 (sc-752; Santa Cruz Biotechnology), anti-CDC7 (sc-56274; Santa Cruz Biotechnology), anti-DBF4 (ab124707; Abcam), and anti-GAPDH (MAB374; Chemicon). Immunoblotted proteins were visualized by chemiluminescence.
Iwai K., Nambu T., Dairiki R., Ohori M., Yu J., Burke K., Gotou M., Yamamoto Y., Ebara S., Shibata S., Hibino R., Nishizawa S., Miyazaki T., Homma M., Oguro Y., Imada T., Cho N., Uchiyama N., Kogame A., Takeuchi T., Kurasawa O., Yamanaka K., Niu H, & Ohashi A. (2019). Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Science Advances, 5(5), eaav3660.
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2

Comprehensive Antibody and Drug Panel for Neurobiological Research

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The following primary antibodies were used: Anti-MeCP2 (Cell Signaling, 3456, 1:2,000), Anti-MeCP2 (Abcam, ab50005, 1:2,000), Anti phospho-S421 (custom made by Covance, 1:2,000), anti-β-Actin (Sigma-Aldrich, a5441, 1:5,000), anti-AURKB (Millipore, 04-1036, 1:1,000; Cell Signalling, 3094, 1:1,000), anti-DLL1(Abcam, Ab84620, 1:1,000), anti-NICD(Cell Signaling, 4147, 1:1,000), anti-phospho-Histone H3 (Ser10)(Cell Signalling, 3377, 1:1,000), anit-Histone H3 (Active Motif, 39163, 1:10,000), anti-S100b (SWANT, 1:500), anti-NeuN (Millipore, MAB377, 1:1,000), anti-BrdU (Accurate Chemical & Scientific, H7786, 1:1,000), anti-Ki67 (Dako, M7248, 1:500), anti-Tuj1 (Progema, G7121, 1:1,000), anti-MAP2(Abcam, ab32454, 1:1,000), anti-GFAP (Dako, Z0334, 1:1,000), anti-Nestin (Aves labs, NES, 1:500), anti-SOX2 (Millipore, MAB4343, 1:1,000), anti-MCM2 (Santa Cruz, sc-9839, 1:1,000). DyLight 680/800 conjugated secondary antibodies (Thermo Fisher Scientific) were used for Western blot. Alexa Fluor conjugated secondary antibodies (Invitrogen) were used for immunofluorescence staining. The following drugs were used: DAPT(Sigma-Aldrich), nocodazole (Sigma-Aldrich), colchicine (Sigma-Aldrich), roscovitine (Calbiochem), nimodipine (Sigma-Aldrich), Myr-CaMK IINtide (Calbiochem), STO-609 (Tocris), Bay K8644 (Calbiochem), Hesperadin (Selleckchem)
Li H., Zhong X., Chau K.F., Santistevan N.J., Guo W., Kong G., Li X., Kadakia M., Masliah J., Chi J., Jin P., Zhang J., Zhao X, & Chang Q. (2014). Cell cycle-linked MeCP2 phosphorylation modulates adult neurogenesis involving the Notch signaling pathway. Nature communications, 5, 5601.
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3

Detecting MCM Proteins in Glioma Tumors

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To detect MCM2, MCM3 and MCM7 protein in glioma tumors, we examined six glioma samples (two each of WHO stages II, III, IV). The protein concentration of cell lysates was determined by the Bio-Rad Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions. Thirty micrograms of protein per tissue lysate was electrophoresed on 7-12% gels and transferred to a polyvinylidene difluoride membrane filter (Millipore, Billerica, MA, USA). Blots were probed with the appropriate antibody. Quantitative determination of the protein was assessed by densitometric scanning of the band from film. An AlphaImager 2000 Documentation and Analysis System (Alpha Innotech Corp, San Leandro, CA, USA) was used to quantify bands of appropriate sizes.
The following primary antibodies were used: anti-β-actin (Sigma Chemical Co, St. Louis MO, USA), anti-MCM2 (sc-10771, Santa Cruz Biotechnology, USA), anti-MCM3 (sc-9849, Santa Cruz Biotechnology, USA), and anti-MCM7 (sc-22782, Santa Cruz Biotechnology, USA).
Hua C., Zhao G., Li Y, & Bie L. (2014). Minichromosome Maintenance (MCM) Family as potential diagnostic and prognostic tumor markers for human gliomas. BMC Cancer, 14, 526.
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4

Antibody Characterization for DNA Replication

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Antibodies against Treslin, MTBP, and TopBP1 were described (Boos et al, 2011 (link), 2013 (link); Köhler et al, 2019 (link)). Anti-BrdU-FITC (556028; Becton Dickinson); anti-HA (mouse, 16B12; Covance); anti-GFP nanobodies (kind gift from Kirill Alexandrov); anti-GFP (mouse, JL-8, 632381; Clonetech), anti-Mcm2 (goat, sc-9839; Santa Cruz Biotechnology), anti-Mcm5 (rabbit, ab17967; Abcam), anti-Cdc45 (rat, 3G10; kind gift from Helmut Pospiech), anti-PCNA (mouse, sc-56; Santa Cruz Biotechnology), NHS (N-hydroxysuccinimide) sepharose (10343240; Thermo Fisher Scientific), and Protein G magnetic beads (10004D; Life Technologies).
Ferreira P., Sanchez-Pulido L., Marko A., Ponting C.P, & Boos D. (2022). Refining the domain architecture model of the replication origin firing factor Treslin/TICRR. Life Science Alliance, 5(5), e202101088.
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5

Western Blot Analysis of Cell Signaling Proteins

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Cancer cells were collected and lysed in RIPA lysis buffer supplemented with protease and phosphatase inhibitors. Protein concentration was measured by BCA assay. Samples in SDS sample buffer were heat-denatured at 95 °C for 5 min. The proteins were separated by SDS-PAGE, transferred onto a nitrocellulose membrane, and blotted with primary antibodies including anti-MCM2 (sc-373702, 1:1000 dilution), anti-MCM3 (sc-390480, 1:1000 dilution), anti-MCM4 (sc-28317, 1:1000 dilution), anti-MCM6 (sc-393618, 1:1000 dilution), anti-AR (sc-7305, 1:1000 dilution), anti-SYP (sc-17750, 1:2000 dilution), anti-CD56 (sc-7326, 1:500 dilution), anti-cleaved PARP1 (sc-56196, 1:1000 dilution), anti-β-Actin (sc-47778, 1:2000 dilution), and anti-GAPDH (sc-47724, 1:2000 dilution) purchased from Santa Cruz Biotechnology. Secondary HRP conjugated antibody (PI31432, 1:6000 dilution) was purchased from Fisher Scientific. Western blot development and detection was performed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific) and IVIS Lumina optical imaging system (PerkinElmer). All the raw images of Western blot were summarized in Supplementary Fig. 4.
Hsu E.C., Shen M., Aslan M., Liu S., Kumar M., Garcia-Marques F., Nguyen H.M., Nolley R., Pitteri S.J., Corey E., Brooks J.D, & Stoyanova T. (2021). MCM2-7 complex is a novel druggable target for neuroendocrine prostate cancer. Scientific Reports, 11, 13305.
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6

Immunoblotting of Cell Signaling Proteins

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Immunoblotting was performed as described previously (44 (link)). The following antibodies were used at 0.1 to 0.5 μg/ml: anti-pMCM2 (3378-1; Epitomics Inc.), anti-MCM2 (sc-9839; Santa Cruz Biotechnology), pChk1 (#2348; Cell Signaling Technology), anti-Chk1 (#2345; Cell Signaling Technology), anti-pCDK1 (#9111; Cell Signaling Technology), anti-CDK1 (sc-954; Santa Cruz Biotechnology), anti-pBRCA1 (#9009; Cell Signaling Technology), anti-pp53 (#9286; Cell Signaling Technology), anti-pATM (2152-1; Epitomics Inc.), anti-p21 (#2947; Cell Signaling Technology), anti-gH2AX (#2577; Cell Signaling Technology), anti-p53 (sc-126; Santa Cruz Biotechnology), anti-PARP1 (#9542; Cell Signaling Technology), anti-BRCA1 (sc-6954; Santa Cruz Biotechnology), anti-CDC7 (sc-56274; Santa Cruz Biotechnology), anti-DBF4 (ab124707; Abcam), and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (MAB374; Chemicon). Immunoblotted proteins were visualized using chemiluminescence.
Iwai K., Nambu T., Kashima Y., Yu J., Eng K., Miyamoto K., Kakoi K., Gotou M., Takeuchi T., Kogame A., Sappal J., Murai S., Haeno H., Kageyama S.I., Kurasawa O., Niu H., Kannan K, & Ohashi A. (2021). A CDC7 inhibitor sensitizes DNA-damaging chemotherapies by suppressing homologous recombination repair to delay DNA damage recovery. Science Advances, 7(21), eabf0197.
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7

Comprehensive Antibody and Drug Panel for Neurobiological Research

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The following primary antibodies were used: Anti-MeCP2 (Cell Signaling, 3456, 1:2,000), Anti-MeCP2 (Abcam, ab50005, 1:2,000), Anti phospho-S421 (custom made by Covance, 1:2,000), anti-β-Actin (Sigma-Aldrich, a5441, 1:5,000), anti-AURKB (Millipore, 04-1036, 1:1,000; Cell Signalling, 3094, 1:1,000), anti-DLL1(Abcam, Ab84620, 1:1,000), anti-NICD(Cell Signaling, 4147, 1:1,000), anti-phospho-Histone H3 (Ser10)(Cell Signalling, 3377, 1:1,000), anit-Histone H3 (Active Motif, 39163, 1:10,000), anti-S100b (SWANT, 1:500), anti-NeuN (Millipore, MAB377, 1:1,000), anti-BrdU (Accurate Chemical & Scientific, H7786, 1:1,000), anti-Ki67 (Dako, M7248, 1:500), anti-Tuj1 (Progema, G7121, 1:1,000), anti-MAP2(Abcam, ab32454, 1:1,000), anti-GFAP (Dako, Z0334, 1:1,000), anti-Nestin (Aves labs, NES, 1:500), anti-SOX2 (Millipore, MAB4343, 1:1,000), anti-MCM2 (Santa Cruz, sc-9839, 1:1,000). DyLight 680/800 conjugated secondary antibodies (Thermo Fisher Scientific) were used for Western blot. Alexa Fluor conjugated secondary antibodies (Invitrogen) were used for immunofluorescence staining. The following drugs were used: DAPT(Sigma-Aldrich), nocodazole (Sigma-Aldrich), colchicine (Sigma-Aldrich), roscovitine (Calbiochem), nimodipine (Sigma-Aldrich), Myr-CaMK IINtide (Calbiochem), STO-609 (Tocris), Bay K8644 (Calbiochem), Hesperadin (Selleckchem)
Li H., Zhong X., Chau K.F., Santistevan N.J., Guo W., Kong G., Li X., Kadakia M., Masliah J., Chi J., Jin P., Zhang J., Zhao X, & Chang Q. (2014). Cell cycle-linked MeCP2 phosphorylation modulates adult neurogenesis involving the Notch signaling pathway. Nature communications, 5, 5601.
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8

Immunoblotting of Replication Proteins

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Anti‐Mcm2 (yN‐19, sc‐6680, Santa Cruz), anti‐Mcm4 (yC‐19, sc‐6685 Santa Cruz), anti‐Mcm5 (yN‐19, sc‐6686, Santa Cruz), anti‐Mcm7 (yN‐19, sc‐6688, Santa Cruz), anti‐Orc6 (SB49), anti‐Cdc6 (9H8/5). FLAG‐tagged proteins were detected with anti‐FLAG M2 (Sigma). Mcm3 was detected with anti‐CBP antibody (07‐482, Merck Millipore). Polyclonal antibodies against Mcm6, Sld3, Sld7 and Cdc45 were described previously (On et al, 2014). Psf1 antibodies were a gift from K. Labib.
Deegan T.D., Yeeles J.T, & Diffley J.F. (2016). Phosphopeptide binding by Sld3 links Dbf4‐dependent kinase to MCM replicative helicase activation. The EMBO Journal, 35(9), 961-973.
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9

Western Blot Analysis of Cell Proteins

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Protein extracts were separated by SDS-PAGE or NuPAGE (Invitrogen) and transferred to nitrocellulose membranes BA-85 (Whatman) for probing. Antibodies used were: anti-actin (Millipore, Mab1501 clone C4), anti-MCM2 (Santa Cruz, SC-6680), anti-tubulin (Thermo Fisher Scientific: MA1-80017), and anti-MYC (9E10).
Shimada K., Loon B.v., Gerhold C., Bregenhorn S., Hurst V., Roth G., Tarashev C., Heinis C., Jiricny J., & Gasser S.M. (2020). Uncoordinated long-patch base excision repair at juxtaposed DNA lesions generates a lethal accumulation of double-strand breaks.
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