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Geneprint 10 kit

Manufactured by Promega
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The GenePrint 10 kit is a genetic analysis tool used to amplify and detect specific DNA regions. It provides a standardized set of genetic markers for human identification and relationship testing.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) L glutamine, by Thermo Fisher Scientific (3 mentions) Mycoalert mycoplasma detection kit, by Lonza (3 mentions) Skov3, by American Type Culture Collection (3 mentions) Genemapper v4, by Thermo Fisher Scientific (3 mentions) Abi prism 3730xl genetic analyzer, by Promega (3 mentions) Rpmi 1640, by Thermo Fisher Scientific (3 mentions) Azd 2281 olaparib, by AstraZeneca (2 mentions) Fetal bovine serum (fbs), by Corning (2 mentions) Mycoalert plus mycoplasma detection kit, by Lonza (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Rpmi 1640, by Nacalai Tesque (1 mentions) Penicillin streptomycin, by American Type Culture Collection (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by American Type Culture Collection (1 mentions) Hff 1, by American Type Culture Collection (1 mentions) L glutamine, by American Type Culture Collection (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Capricorn (1 mentions)

Spelling variants of this product

GenePrint 10 (30 citations) GenePrint 10 System kit (27 citations) Geneprint Kit (5 citations) GenePrint 10 System PCR Amplification kit (3 citations) GenePrint 10.0 kit (2 citations) GenePrint 10 STR profiling kit (2 citations)

43 protocols using geneprint 10 kit

1

Cell Line Authentication and Maintenance

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All lung carcinoma cell lines except PC-9 were obtained directly from the American Type Culture Collection (ATCC) repository. The PC-9 cell line was a gift from A. Ali (CSI, Singapore). Cell lines were authenticated by DNA short tandem repeats analysis using GenePrint 10 kit (Promega). The cell lines were maintained in RPMI-1640 (Nacalai Tesque) growth medium supplemented with 10% fetal bovine serum (FBS, HyClone, Thermo Scientific), 2 mM L-glutamine (Gibco, Life Technologies) and 100 U/ml penicillin-streptomycin (Gibco, Life Technologies) at 37°C in a 5% CO2 atmosphere.
Chua K.N., Kong L.R., Sim W.J., Ng H.C., Ong W.R., Thiery J.P., Huynh H, & Goh B.C. (2015). Combinatorial treatment using targeted MEK and SRC inhibitors synergistically abrogates tumor cell growth and induces mesenchymal-epithelial transition in non-small-cell lung carcinoma. Oncotarget, 6(30), 29991-30005.
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2

Cell Line Authentication and Maintenance

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RMS cell lines RH30, RD, RMS-YM, RH4, JR-1, RMS-01, RH41, HFF-1 have been described previously [17 (link)]. Cell lines were cultured in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) (RD, RH30, RMS-01, RH4) or Roswell Park Memorial Institute 1640 (RPMI) medium (Thermo Fisher Scientific, Waltham, MA, USA)(RH41, RMS-YM) supplemented with 10% fetal calf serum (Gibco, Life Technologies Ltd., Thermo Fisher Scientific, Waltham, MA, USA), 2 mM L-glutamine (Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). HFF-1 cells were purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium supplemented with 15% fetal calf serum, 2 mM L-glutamine and 1% penicillin/streptomycin. Cells were maintained at 37 °C at 5% CO2.
Cell lines were authenticated using the Geneprint 10 kit according to manufacturer’s instructions (Promega, Madison, WI, USA) and subsequent fragment length analysis was carried out by Eurofins Genomics (Ebersberg, Germany). The resulting short tandem repeat (STR) typing results were compared with public databases.
Walters Z.S., Aladowicz E., Villarejo-Balcells B., Nugent G., Selfe J.L., Eve P., Blagg J., Rossanese O, & Shipley J. (2021). Role for the Histone Demethylase KDM4B in Rhabdomyosarcoma via CDK6 and CCNA2: Compensation by KDM4A and Apoptotic Response of Targeting Both KDM4B and KDM4A. Cancers, 13(7), 1734.
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3

Isogenic MCF10 Cell Line Series

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The isogenic MCF10 cell line series includes the initial untransformed normal cell line, MCF10A, the benign proliferation stages (MCF10AT1 and MCF10NeoT), the carcinoma in situ stage (MCF10DCIS.com), and the invasive carcinoma stages (MCF10Ca1a cl1, MCF10Ca1d cl1, and MCF10Ca1h). Cell lines were kindly provided by The Barbara Ann Karmanos Cancer Institute (Detroit, MI, USA), except for MCF10A cells, which were purchased from The American Type Culture Collection (LGC, Teddington, UK), and MCF10DCIS.com cells from Asterand (Royston, UK). Cells were authenticated by short tandem repeat typing with the Geneprint10 Kit (Promega, Southampton, UK), and routinely tested for mycoplasma infection by use of an enzyme‐linked immunosorbent assay‐based test (MycoAlert Mycoplasma detection kit; Lonza, Basel, Switzerland). Cells were grown as described previously 30 and in supplementary materials, Supplementary materials and methods.
Maguire S.L., Peck B., Wai P.T., Campbell J., Barker H., Gulati A., Daley F., Vyse S., Huang P., Lord C.J., Farnie G., Brennan K, & Natrajan R. (2016). Three‐dimensional modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 model. The Journal of Pathology, 240(3), 315-328.
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4

Cell Line Authentication and Modification

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BCPAP (65 (link)) and K1 (66 ) cells were purchased from ATCC in 2014 and maintained in RPMI supplemented with 10% fetal bovine serum, 1% penicillin, and 1% streptomycin. The identity of both cell lines was confirmed by DNA profiling of polymorphic short tandem repeat (STR) markers through the human cell line authentication analysis service at the Duke University DNA Analysis Facility. The resultant STR makers, assess by GenePrint 10 kit (Promega), were compared to those available for BCPAP (CVCL_0153) and K1 (CVCL_2537) cells lines through Cellosaurus (65 (link)) on February 2018. Both cell lines were also confirmed to be free of mycoplasma infection, as assessed by the Duke Cell Culture Facility using MycoAlert PLUS test (Lonza) on January 2018. Both cell lines were used within 5 passages of being thawed. BCPAP cell lines were engineered to express ERK2GOF by stable infection using established methods (67 (link)) of a retrovirus derived from the plasmid pBABEpuro-HA-ERK2GOF encoding the ERK2R67S, D321N mutant form of ERK2, termed ERKGOF (40 (link)).
Xu M., Casio M., Range D.E., Sosa J.A, & Counter C.M. (2018). Copper chelation as targeted therapy in a mouse model of oncogenic BRAF-driven papillary thyroid cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(17), 4271-4281.
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5

HPV-Negative Cell Line Authentication Protocol

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The five HPV-negative cell lines Cal27, Cal33, UPCI:SCC040, UPCI:SCC131, UPCI:SCC099 (ATCC, Wesel and DSMZ, Braunschweig, Germany) were cultured in DMEM (Life Technologies, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Capricorn, Ebsdorfergrund, Germany) and maintained at 37°C in a humidified 5% CO2 atmosphere. The GenePrint 10 kit (Promega, Walldorf, Germany) and GeneMapper 5.0 software were used for short-tandem repeat analysis. To confirm the authenticity of all cell lines, the data were compared to Expasy and DSMZ databases (30 (link)). Cell lines are routinely tested free from mycoplasma contamination using a PCR-based assay (31 (link)).
Tiwari D.K., Hannen R., Unger K., Kohl S., Heß J., Lauber K., Subtil F.S., Dikomey E., Engenhart-Cabillic R, & Schötz U. (2022). IL1 Pathway in HPV-Negative HNSCC Cells Is an Indicator of Radioresistance After Photon and Carbon Ion Irradiation Without Functional Involvement. Frontiers in Oncology, 12, 878675.
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6

Authenticated Cell Line Characterization

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HMT3522 T4-2 (human breast cancer derived; from Valerie Weaver), NCI-H1299 (human lung cancer derived; from ATCC), HEK293-FT (human kidney derived; from Thermo Fisher Scientific). All cell lines used in this study were authenticated through STR profiling using Promega Geneprint 10 Kit. Gene fragment analysis was performed on a 3130xl Genetic Analyser, and Genemapper v5 was used for analysis. Cell lines were confirmed negative for mycoplasma.
Nászai M., Bellec K., Yu Y., Román-Fernández A., Sandilands E., Johansson J., Campbell A.D., Norman J.C., Sansom O.J., Bryant D.M, & Cordero J.B. (2021). RAL GTPases mediate EGFR-driven intestinal stem cell proliferation and tumourigenesis. eLife, 10, e63807.
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7

Ovarian Cancer Cell Line Characterization

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The epithelial ovarian cancer cell lines SKOV-3, UWB1.289+BRCA1 wild-type BRCA1 wild type (Brca1 WT) and UWB1.289 BRCA1 null (Brca1 Null) cell lines (American Type Culture Collection, Manassas, VA), OVCAR-8 and NCI/ADR-RES (National Cancer Institute, Bethesda, MD) were maintained in culture as previously described [23 (link), 24 (link)]. The SKOV-3, OVCAR-8 and NCI/ADR-RES cell lines are represented in the National Cancer Institute 60 (NCI 60) Cancer Panel [25 (link), 26 (link)], and the BRCA1 WT and BRCA null cell lines have been well described [27 ]. Cell lines were authenticated in December 2013 by the Vanderbilt VANTAGE Genomics Core using the GenePrint 10 kit (Promega, Madison, WI). Gene loci profiles were verified using, where applicable, NCI 60 (25, 26), COGCELL, and ATCC reference databases. All cell lines used tested negative for mycoplasma. Cells were treated with 0.01% DMSO as vehicle control; vorinostat (SAHA) (synthesized at the Broad Institute, Cambridge, MA); AZD-2281 (olaparib) (Astra Zeneca Pharmaceuticals, Wilmington, DE); and the combination of SAHA and olaparib.
Konstantinopoulos P.A., Wilson A.J., Saskowski J., Wass E, & Khabele D. (2014). Suberoylanilide Hydroxamic Acid (SAHA) enhances olaparib activity by targeting homologous recombination DNA repair in ovarian cancer. Gynecologic oncology, 133(3), 599-606.
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8

HepG2 Cell Culture and Peptide Treatment

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The HCC line HepG2 (American Type Culture Collection (ATCC) Manassas, VA, USA) was maintained in Dulbecco’s modified Eagle’s media (DMEM) with 4.5 g/l glucose (Corning, Corning, NY), 10% fetal bovine serum (Corning), and 100 U/ml penicillin and streptomycin (Gibco). These cells were authenticated using the GenePrint 10 kit (Promega) to obtain a short tandem repeat (STR) profile, which was then compared to the ATCC STR database. Mycoplasma contamination was not tested. AXT050 was produced by solid-phase synthesis and purchased from New England Peptide. High-performance liquid chromatography and mass spectrometry analysis indicated a purity >90%. For working solutions, the lyophilized peptide was dissolved in 100% DMSO to a concentration of 40 mM and stored at −20 °C until used. For cell-based experiments, aliquots were diluted to 2 mM working stocks in water. Excess dimethyl sulfoxide (DMSO) was added to each culture to normalize the final DMSO concentration to 0.06% in all samples.
Jafarnejad M., Sové R.J., Danilova L., Mirando A.C., Zhang Y., Yarchoan M., Tran P.T., Pandey N.B., Fertig E.J, & Popel A.S. (2019). Mechanistically detailed systems biology modeling of the HGF/Met pathway in hepatocellular carcinoma. NPJ Systems Biology and Applications, 5, 29.
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9

STR Analysis of hEM3 Cell Line

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STR analysis was performed on hEM3 by the Genetic Resources Core Facility, Johns Hopkins University, using a Promega GenePrint 10 Kit. The PCR products were electrophoresed on an ABI Prism® 3730xl Genetic Analyzer using an Internal Lane Standard 600 (Promega). Data were analyzed using GeneMapper® v 4.0 software (Applied Biosystems).
Park Y., Jung J.G., Yu Z.C., Asaka R., Shen W., Wang Y., Jung W.H., Tomaszewski A., Shimberg G., Chen Y., Parimi V., Gaillard S., Shih I.M, & Wang T.L. (2021). A novel human endometrial epithelial cell line for modeling gynecological diseases and for drug screening. Laboratory investigation; a journal of technical methods and pathology, 101(11), 1505-1512.
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10

Standardized STR Profiling of Cell Lines

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STR profiling was carried out following the ANSI/ATCC ASN-0002-2011 guidance, Authentication of Human Cell Lines: Standardization of STR Profiling. Briefly, a Promega GenePrint 10 Kit was used to polymerase chain (PCR) amplify eight short tandem repeat (STR) loci plus a gender-determining marker, Amelogenin. The PCR product was electrophoresed on an ABI Prism® 3730xl Genetic Analyzer using an Internal Lane Standard 600 (Promega). Data was analyzed using GeneMapper version 4.0 software (Applied Biosystems). Appropriate positive and negative controls were used.
All STR experiments have been performed in one laboratory (JHU) by one individual to exclude any possible inter-laboratory and/or inter-operator effects on reproducibility.
Data and materials availability. The CGH datasets have been deposited in the Gene Expression Omnibus (GEO) under GSE80760 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80760). The transcriptomics microarray datasets have been deposited in the Gene Expression Omnibus (GEO) under GSE77244 (http://www.dtd.nlm.nih.gov/geo/query/acc.cgi?acc=GSE77244).
Kleensang A., Vantangoli M.M., Odwin-DaCosta S., Andersen M.E., Boekelheide K., Bouhifd M., Fornace AJ J.r., Li H.H., Livi C.B., Madnick S., Maertens A., Rosenberg M., Yager J.D., Zhaog L, & Hartung T. (2016). Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. Scientific Reports, 6, 28994.
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