Western blotting was carried out as previously described [27 (link)]. The primary antibodies used were anti-N-Cadherin (BD Biosciences, Breda, Netherlands #610920), anti-α-Smooth Muscle Actin (Sigma, Zwijndrecht, Netherlands #A2547), anti-Snail (Cell Signaling, Leiden, Netherlands #3879), anti-Smad2 (BD Biosciences, Breda, Netherlands #610842), anti-p-Smad2 (Cell Signaling, Leiden, Netherlands #3108), anti-Smad4 (Santa Cruz #sc7966), anti-TGFβRI (Santa Cruz, Heidelberg, Germany #sc 398), anti-TGFβRII (Santa Cruz, Heidelberg, Germany #sc-400), anti-Smad3 (Epitomics, Duiven, Netherlands #1735–1), anti-p-Smad3 (a kind gift from Dr Edward B Leof, Mayo Clinic, Rochester, Minnesota) and anti-β-actin (Sigma, Zwijndrecht, Netherlands #A5441). All the secondary antibodies were from Sigma, Zwijndrecht, Netherlands. Western quantification was performed using image J software.
Anti smad2
Manufactured by BD
Sourced in Netherlands, United States
Anti-Smad2 is a laboratory equipment product that functions as an antibody. It is designed to detect and quantify the Smad2 protein, which is a key component of the TGF-beta signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti α smooth muscle actin, by Merck Group (1 mentions)
Anti smad4, by Santa Cruz Biotechnology (1 mentions)
Anti tgfβri, by Santa Cruz Biotechnology (1 mentions)
Anti tgf β rii, by Santa Cruz Biotechnology (1 mentions)
Anti smad3, by Abcam (1 mentions)
Anti p smad3, by Merck Group (1 mentions)
Anti snail, by Cell Signaling Technology (1 mentions)
Anti p smad2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti n cadherin, by BD (1 mentions)
Amersham hyperfilm ecl, by GE Healthcare (1 mentions)
2 protocols using anti smad2
1
Western Blotting of EMT Markers
2
Western Blot Analysis of SMAD Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell lysates were determined by a bicinchoninic acid (BCA) protein assay kit. The proteins were subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). After that, the gels were transferred to polyvinylidene difluoride (PVDF) membranes, which were incubated with rabbit polyclonal anti-SMAD2 (dilution 1:800), anti-SMAD3 (1:1,000), anti-SMAD7 (1:1,200), and anti-GAPDH (1:600) antibodies (all BD, Franklin Lakes, NJ, USA) overnight at 4°C. After washing with PBS three times, the membranes were incubated with a secondary polyclonal peroxidase labeled antibody for 2 h, and detected using autoradiography films (Amersham HyperFilm ECL; GE Healthcare, Fairfield, Connecticut, USA). Quantification was performed on Quantity One.
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