Sodium cyanide

Sodium cyanide of 95% purity was procured from Loba Chemie Pvt. Ltd., Mumbai, India. Stock solution was prepared by dissolving Sodium cyanide in double distilled water in a standard volumetric flask. Water was renewed every day over test periods. Henceforth, the replacement of the water medium was followed by the addition of the desired dose of the test compound. The fish were exposed in batches of 10 to a fixed concentration of Sodium cyanide with 20 L of water in three replicates for each concentration. One tenth (0.1 mg/L) of the 96 h LC₅₀ (1mg/L) was selected as sub lethal concentration for studies and the durations of exposure were 10 and 20 days. Further, the fish were allowed to undergo a recovery period of 14 days. This study was conducted under the guidelines issued by Organization for economic co-operation and development (OECD, 1992) for static-renewal test conditions. At the end of 10 and 20 days of exposure and that of 14 days of recovery, the fish were sacrificed and sampled for histopathological studies.

Sodium cyanide of 95% purity was procured from Loba Chemie Pvt., Ltd., Mumbai, India. All other chemicals used in the current study were of analytical grade and commercially available.