Denville 260d

Manufactured by Thomas Scientific

The Denville 260D is a laboratory centrifuge designed for general-purpose applications. It features a brushless motor, digital speed and time controls, and an easy-to-use interface. The centrifuge can accommodate a variety of rotor types and sample volumes, making it a versatile piece of equipment for various laboratory tasks.

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Lab products found in correlation

Quantichrom α amylase assay kit, by BioAssay Systems (1 mentions) Cobas fara centrifugal analyser, by Roche (1 mentions)

Close spelling variants of this product

Denville Micro 260D Microcentrifuge

2 protocols using denville 260d

Optimized TTR Fibrillation for SAXS

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Prior to the initiation of TTR fibrillation process, the sample was centrifuged for 60 s at 13,400 rpm (Denville 260D, Denville Scientific Inc). The experimental conditions for the fibrillation reaction were optimized such as to allow for SAXS data collection from adequately concentrated protein samples, within a time-window of maximally 24 hours38 (link). The fibrillation of 5 mg/ml TTR was induced by adding a stock solution of 1 M acetic acid, 2 M NaCl to a final concentration of 50 mM acetic acid and 100 mM NaCl (final pH 3.0). The sample was mixed using a Vibromix (Practical Science, Nederlands) for 5 s placed in a waterbath at 4 °C. Aliquots were taken as needed for further analysis. Mature protofibrils were obtained by incubation at these conditions for 1 month.

Groenning M., Campos R.I., Hirschberg D., Hammarström P, & Vestergaard B. (2015). Considerably Unfolded Transthyretin Monomers Preceed and Exchange with Dynamically Structured Amyloid Protofibrils. Scientific Reports, 5, 11443.

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Plasma and Serum Amylase Analysis

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At 4.5 hours after AP induction blood was collected from the femoral artery catheter in a heparinzed syringe and centrifuged (Denville 260D; Denville Scientific; Metuchen, NJ) at 3000 rpf for 10 min at 4 °C to generate plasma. Amylase was measured by ELISA (QuantiChrom α-amylase Assay Kit, BioAssay Systems, Hayward, CA) according the manufacturer's instructions and expressed as IU/L. Mouse blood was left at room temperature for 30 minustes then microcentrifuged at 5000 × g for 10 minutes to generate serum. Serum aliquots were stored at -80 °C. Serum amylase activity was measured using a commercial kit (Alpha Laboratories Ltd., Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd, Welwyn Garden City, UK.).

Fishman J.E., Levy G., Alli V., Zheng X., Mole D.J, & Deitch E.A. (2014). The intestinal mucus layer is a critical component of the gut barrier that is damaged during acute pancreatitis. Shock (Augusta, Ga.), 42(3), 264-270.

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