Epicatechin gallate

The 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2, 4, 6-Tris(2-pyridyl)-s-triazine (TPTZ), 2, 6-di-tert-butyl-4-methylphenol (BHT), vitamin C (Vc), were obtained from Solarbio Life sciences (Beijing, China). All chemical reagents including methanol, ethyl acetate (EtOAc), petroleum ether (PE), Dichloromethane (DCM), and n-butanol (n-BuOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). Folin-Ciocalteu, pyrogallol, and other analytical grade chemicals were purchased from Aladdin (Shanghai, China). Myricetin, dihydroMyricetin, quercetin, resveratrol, apigenin, baicalein, hesperitin, gallic acid, rutin, luteolin, epicatechin, epicatechin gallate, and other standards for HPLC were purchased from Yuanye Bio-Technology (Shanghai, China). Cellulase (3, 000 μ/g) and pectinase (40, 000 μ/g) were purchased from Xiya reagent (Shandong, China). α-Glucosidase (G5003) and acetylcholinesterase (AChE, C3389) were purchased from Sigma-Aldrich (St. Louis, USA). p-Nitrophenol nitrophenol α-D-glycopyranoside (pNPG), acarbose, galantamine, and cisplatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HepG2, MCF-7, Raw264.7, and A549 cells were purchased from Conservation genetics CAS Kunming cell bank.

Fu brick tea (Camellia sinensis L.) was obtained from a dark tea factory in Yiyang city, Hunan province, China. The glutinous rice (Oryza sativa var.) was bought from a local supermarket.
The compounds 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris (2-pyridyl)-S-triazine (TPTZ), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), ferrozine, ascorbic acid (vitamin C), 1,10-phenanthroline, and pUC18 plasmid DNA were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The standards: gallic acid (GA), (−)-gallocatechin (GC), theobromine (Tb), 3,4-dihydroxybenzoic acid (DbA), theophylline (Tp), (−)-epigallocatechin (EGC), 3,4-dihydroxybenzaldehyde (Dbd), caffeine, catechin (C), caffeic acid (CA), (−)-epicatechin (EC), (−)-epigallocatechin gallate (EGCG), (−)-gallocatechin gallate (GCG), and (−)-epicatechin gallate (ECG) were purchased from Yuan-ye Biotech Co. (Shanghai, China). LC-grade methanol, acetonitrile, and trifluoroacetic acid were purchased from Merck (Darmstadt, Germany). Folin–Ciocalteu reagent and all other chemicals were analytical grade and purchased from Sinopharm Chemical Reagent Co. (Shanghai, China).