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Observer 5

Manufactured by Noldus
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Sourced in Netherlands, United States

Observer 5.0 is a software package designed for the collection, analysis, and management of observational data. The software provides a structured and systematic approach to recording and interpreting behavioral observations, without making claims about its intended use or applications.

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Lab products found in correlation

Ethovision 3, by Noldus (3 mentions) Ethovision 14, by Noldus (2 mentions) Middle square, by Panasonic (1 mentions) Ethovision, by Noldus (1 mentions) Ethovision software, by Noldus (1 mentions)

Close spelling variants of this product

Observer-Pro 5.0 system Observer 5.0 software package

27 protocols using observer 5

1

Pup Retrieval Assay in C57BL/6J Mice

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Before behavioural testing animals were housed individually for 5 – 7 d unless otherwise specified. Experiments started at the beginning of the dark phase and were performed under dim red light. Testing was performed in the home cage (with the exception of locomotion assays, see below) and preceded by a 30 min habituation period. Two 1 – 4 d old C57BL/6J pups were placed in different corners opposite the nest. Once retrieval occurred, a timer was started. Each test was recorded using a multi-camera surveillance system (GeoVision GV-1480) and behaviours were scored by an individual blind to the genotype using the Observer 5.0 or XT 8 software (Noldus Information Technology).
Kohl J., Babayan B.M., Rubinstein N.D., Autry A.E., Marin-Rodriguez B., Kapoor V., Miyamishi K., Zweifel L.S., Luo L., Uchida N, & Dulac C. (2018). Functional circuit architecture underlying parental behaviour. Nature, 556(7701), 326-331.
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2

Behavioral Assays in Dim Light

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Before behavior tests animals were housed individually for about one week. Experiments started at the beginning of the dark phase and were performed under dim red light, unless noted otherwise. Each test was videotaped (Sony DCR-HC65 camcorder in nightshot mode, Microsoft LifeCam HD-5000 or Geovision surveillance system) and the behaviors were scored by an individual blind to the genotype using the Observer 5.0 or XT 11 software (Noldus Information Technology). When one animal is tested in multiple behavior assays, they are allowed at least 48 hours rest between tests.
Wu Z., Autry A.E., Bergan J.F., Watabe-Uchida M, & Dulac C.G. (2014). Galanin neurons in the medial preoptic area govern parental behavior. Nature, 509(7500), 325-330.
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3

Elevated Plus Maze Anxiety Assay

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The apparatus was made of grey PVC and elevated 75 cm above the floor. The four arms (50×10 cm2) formed a cross with the central platform. A wall (height: 30 cm) of non-transparent material enclosed two arms, located opposite to each other. Each rat was placed on the central platform facing one of the enclosed arms and allowed to freely explore the maze for 5 min. Behaviour was scored manually using Observer 5.0 (Noldus Information Technology, Wageningen, The Netherlands). The time spent on the open arm of the maze was calculated as a measure of anxiety, whereas the total number of closed arm entries was considered as a measure of general activity.
Homberg J.R., Olivier J.D., VandenBroeke M., Youn J., Ellenbroek A.K., Karel P., Shan L., van Boxtel R., Ooms S., Balemans M., Langedijk J., Muller M., Vriend G., Cools A.R., Cuppen E, & Ellenbroek B.A. (2016). The role of the dopamine D1 receptor in social cognition: studies using a novel genetic rat model­. Disease Models & Mechanisms, 9(10), 1147-1158.
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4

Behavioral Assays in Dim Light

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Before behavior tests animals were housed individually for about one week. Experiments started at the beginning of the dark phase and were performed under dim red light, unless noted otherwise. Each test was videotaped (Sony DCR-HC65 camcorder in nightshot mode, Microsoft LifeCam HD-5000 or Geovision surveillance system) and the behaviors were scored by an individual blind to the genotype using the Observer 5.0 or XT 11 software (Noldus Information Technology). When one animal is tested in multiple behavior assays, they are allowed at least 48 hours rest between tests.
Wu Z., Autry A.E., Bergan J.F., Watabe-Uchida M, & Dulac C.G. (2014). Galanin neurons in the medial preoptic area govern parental behavior. Nature, 509(7500), 325-330.
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5

Nest Male Behavior During Reproduction

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We recorded the behavior of focal nest males during their NHP in order to obtain information about possible changes in activity patterns in the course of holding a nest. The behavior was monitored using a handheld computer in a waterproof housing, equipped with the software program Observer 5.0 (Noldus, Wageningen, the Netherlands). Focal nest males were observed for 7 min twice a day (morning and afternoon) for their entire NHP, while recording the following behavioral categories: (1) inactive: when the nest male remained immobile aboveground or was sitting on the bottom. (2) active: when the nest male was (a) foraging, (b) courting, (c) spawning, (d) aggressive, or (e) exploring (i.e., leaving the nest for unknown activities). Our aim was to conduct behavioral observations of each individual on all days of their experimentally assigned NHP. However, some of the observations were missing due to unexpected incidents (e.g., thunderstorms).
von Kuerthy C., Tschirren L, & Taborsky M. (2015). Alternative reproductive tactics in snail shell-brooding cichlids diverge in energy reserve allocation. Ecology and Evolution, 5(10), 2060-2069.
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6

Sexual Behavior Assessment in Rats

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The sexual behavior test was performed as previously described (Chan et al. 2010 ). Briefly, estrus was induced in female rats with estradiol injection 36 to 42 h before testing. Male rats were placed in an observation cage (30 × 40 × 60 cm) for a 30-min habituation period. Following the habituation period, an estrous female was placed in the cage and video recording commenced for 30 min. The frequencies of mounts (no vaginal penetration), intromissions (vaginal penetration), and ejaculations, and the latency to the first ejaculation (time between first mount to ejaculation) were measured and scored during the 30-min test using Observer® 5.0 (Noldus, Wageningen, The Netherlands). Copulatory efficiency was defined as [number of intromissions / (number of intromissions + number of mounts)] × 100 %. Data from the first ejaculation series, which included all events occurring before the first ejaculation, were reported for mounting frequency, ejaculation latency, and copulatory efficiency. The ejaculation frequency for the entire 30-min test was reported. All assessments were performed in the dark phase of the light/dark cycle under dim red light conditions.
Oosting R.S., Chan J.S., Olivier B., Banerjee P., Choi Y.K, & Tarazi F. (2016). Differential effects of vilazodone versus citalopram and paroxetine on sexual behaviors and serotonin transporter and receptors in male rats. Psychopharmacology, 233, 1025-1034.
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7

Quantitative Evaluation of Marmoset Parkinson's Behavior

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To assess gross motor impairment we recorded 20-minute videos of the males in their home cage twice weekly. Videos were then scored by a trained observer who was blind to the experiment. In order to compare more traditional qualitative PD impairment scores with quantitative evaluations of changes to marmoset species-specific behavior, the videos were scored two ways. First, the scorer assessed the video using a traditional impairment scoring regime in which they watched a 5-min. segment from the middle of the video and gave an impairment score based on their overall impression of the animal movement (PD score). An animal was scored for range of movement, bradykinesia, postural abnormality, and checking behavior using a 0 – 4 ranking scheme (see Table 1; [13 ]). Next the scorer assessed the same video segment using Observer 5.0 (Noldus Information Technology, Leesburg, VA) to record all occurrences of shifts in the animal’s ranking for each behavior assessed in the PD scoring scheme. At the end of the time frame Observer tallied the ranking for each behavior (Quantitative PD Score). Both the PD score and the Quantitative PD score were then weighted using the standard Fox scoring system (Johnston & Fox 2015). Impairment scores from the videos were averaged together for each individual animal as either pre- or post-dose for comparison.
Phillips K.A., Ross C.N., Spross J., Cheng C.J., Izquierdo A., Biju K.C., Chen C., Li S, & Tardif S.D. (2017). Behavioral Phenotypes Associated with MPTP Induction of Partial Lesions in Common Marmosets (Callithrix jacchus). Behavioural brain research, 325(Pt A), 51-62.
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8

Pup Retrieval Assay in C57BL/6J Mice

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Before behavioural testing animals were housed individually for 5 – 7 d unless otherwise specified. Experiments started at the beginning of the dark phase and were performed under dim red light. Testing was performed in the home cage (with the exception of locomotion assays, see below) and preceded by a 30 min habituation period. Two 1 – 4 d old C57BL/6J pups were placed in different corners opposite the nest. Once retrieval occurred, a timer was started. Each test was recorded using a multi-camera surveillance system (GeoVision GV-1480) and behaviours were scored by an individual blind to the genotype using the Observer 5.0 or XT 8 software (Noldus Information Technology).
Kohl J., Babayan B.M., Rubinstein N.D., Autry A.E., Marin-Rodriguez B., Kapoor V., Miyamishi K., Zweifel L.S., Luo L., Uchida N, & Dulac C. (2018). Functional circuit architecture underlying parental behaviour. Nature, 556(7701), 326-331.
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9

Piglet Behavior During Weaning

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On the day of weaning, and 15 and 27 days after weaning, behaviours of weaned piglets were scored live using 2-min instantaneous scan sampling for 6 h/day during 6 sessions of 1 h (8:00–9:00 h, 9:15–10:15 h, 10:30–11:30 h, 14:00–15:00 h, 15:15–16:15 h, 16:30–17:30 h). The behaviours (S1 Table) were recorded using a Psion hand-held computer with the Observer 5.0 software package (Noldus Information Technology B.V., Wageningen, The Netherlands).
Clouard C., Gerrits W.J., Kemp B., Val-Laillet D, & Bolhuis J.E. (2016). Perinatal Exposure to a Diet High in Saturated Fat, Refined Sugar and Cholesterol Affects Behaviour, Growth, and Feed Intake in Weaned Piglets. PLoS ONE, 11(5), e0154698.
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10

Multiparametric Holeboard Behavioral Assay

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The mHB combines the features of an OF, a hole board test and a LD. A detailed description of the test set-up can be found in Labots et al. [32] (link). In the conventional light regime, a stage light above the mHB lit the board to induce a light intensity difference of about 300 lx between the protected area (150 lx, box) and the unprotected area (450 lx, board). The light intensity difference was about 115 lx in the reverse light regime, where the board was lit with 120 lx and the Box 1-5 lx. The animals were free to explore the arena for 5 min. Several parameters being indicative for anxietyrelated behavior, exploration and locomotion were scored by a trained observer using the program Observer 5.0 (Noldus Information Technology, Wageningen, The Netherlands). The tests were also videotaped for raw data storage. An overview of the parameters can be found in Supplementary Table A.1. The apparatus was cleaned with water and tissues between every trial to avoid a bias based on mice olfactory cues.
Labots M., Zheng X., Moattari G., Lozeman-Van't Klooster J.G., Baars J.M., Hesseling P., Lavrijsen M., Kirchhoff S., Ohl F, & van Lith H.A. (2016). Substrain and light regime effects on integrated anxiety-related behavioral z-scores in male C57BL/6 mice-Hypomagnesaemia has only a small effect on avoidance behavior. Behavioural brain research, 306.
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