Ms hplc system

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The MS HPLC system is a high-performance liquid chromatography (HPLC) instrument coupled with a mass spectrometer (MS) for analytical applications. The system separates and detects chemical compounds within a sample, providing detailed information about their molecular composition.

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Lab products found in correlation

Surveyor autosampler, by Thermo Fisher Scientific (3 mentions) Ltq linear ion trap mass spectrometer, by Thermo Fisher Scientific (3 mentions) Msq plus, by Thermo Fisher Scientific (1 mentions) Lpg 3400rs pump, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Thermo HPLC-MS system Surveyor MS Pump Plus HPLC system HPLC-MS HPLC-MS/MS HPLC system

5 protocols using ms hplc system

Beetroot Extract Compound Identification

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For the identification of the compounds in the beetroot extract, a Thermo HPLC-MS system was used, equipped with a Diode Array and MSQ Plus detector, an Acclaim 120 C18 column (150 mm × 4.6 µm), acetonitrile (5–95%), and formic acid 0.1% (95–5%), with a flow rate of 0.4 mL/min as mobile phase and 10 µL injection volume. The MSQ Plus functioned in ESI mode (positive ion mode), with 250 °C probe temperature, and +75 V (cone). The UV absorptions were recorded at 4 wavelengths: 310 nm, 480 nm, 505 nm, and 541 nm.

Popescu V., Blaga A.C., Pruneanu M., Cristian I.N., Pîslaru M., Popescu A., Rotaru V., Crețescu I, & Cașcaval D. (2021). Green Chemistry in the Extraction of Natural Dyes from Colored Food Waste, for Dyeing Protein Textile Materials. Polymers, 13(22), 3867.

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HPLC-MS Analysis of Bioactive Fractions

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EtOAc and BuOH fractions were analysed by HPLC-MS system (Thermo Fisher Scientific, Germany) with an LPG3400RS pump, pressure 290 -15000 psi; automatic injection WPS3000TRS; Electrospray ionization, Ion Trap LCQ FLEET (Thermo Fisher Scientific, Germany). Column Ascentis Express C18 (2.7 µm, 10 cm x 4.6 mm, Waters) was maintained at 25 °C. The eluents were H2O adjusted 0,01 % H3PO4 (A) and methanol (MeOH, B). The mobile phase (v/v) was used in gradient mode: 0-5 min, A-B

do Carmo P.L., Mello R.J., Ramos I.F.O., de Jesus C.C.M., Pontes R.G.M.D.S., de Araújo M.H., Bonavita A.G.C., Pinto S.C., Muzitano M.F, & Raimundo J.M. (2023). Analgesic and anti-inflammatory activities of ethyl acetate and butanol fractions from Vitex polygama hydroalcoholic leaf extract. Natural product research, 37(5).

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Liquid Chromatography-Tandem Mass Spectrometry

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The digested samples were analyzed using a Thermo-Finnigan linear ion-trap (LTQ) mass spectrometer coupled with a Surveyor autosampler and MS HPLC system (Thermo-Finnigan). Tryptic peptides were injected onto the C18 microbore RP column (Zorbax SB-C18, 1.0 mm x 150 mm) at a flow rate of 50 μL/min. The mobile phases A, B, and C were 0.1% formic acid in water, 50% ACN with 0.1% formic acid in water, and 80% ACN with 0.1% formic acid in water, respectively. The gradient elution profile was as follows: 10% B (90% A) for 5 min, 10-95% B (90-5% A) for 120 min, 100% C for 5 min, and 10% B (90% A) for 12 min. The data were collected in the “Triple-Play” (MS scan, Zoom scan, and MS/MS scan) mode with the ESI interface using normalized collision energy of 35%. Dynamic exclusion settings were set to repeat count 1, repeat duration 30 s, exclusion duration 120 s, and exclusion mass width 0.75 m/z (low) and 2.0 m/z (high). Each sample was injected twice.

Lai X., Liangpunsakul S., Li K, & Witzmann F.A. (2015). Proteomic profiling of human sera for discovery of potential biomarkers to monitor abstinence from alcohol abuse. Electrophoresis, 36(4), 556-563.

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HK-2 Cell Proteome Profiling by LFQMS

Cited 1 time

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Triplicate protein extracts from HK-2 cells, treated with THP or vehicle, were analyzed using LFQMS as previously described (18 (link), 19 (link)). In brief, HK-2 proteins were digested with trypsin and peptides analyzed using a Thermo-Finnigan linear ion-trap (LTQ) mass spectrometer coupled with a Surveyor autosampler and MS HPLC system (Thermo-Finnigan). The data were collected in the “Data dependent MS/MS” mode with the ESI interface and searched against the International Protein Index (IPI) HUMAN database using SEQUEST (v. 28 rev. 12) algorithms in Bioworks (v. 3.3). The searched peptides and proteins were validated by PeptideProphet (64 (link)) and ProteinProphet (65 (link)) in the Trans-Proteomic Pipeline (TPP, v. 3.3.0) (http://tools.proteomecenter.org/software.php). Only proteins with probability ≥ 0.9000 and peptides with probability ≥ 0.8000 were used for quantitation.

LaFavers K.A., Macedo E., Garimella P.S., Lima C., Khan S., Myslinski J., McClintick J., Witzmann F.A., Winfree S., Phillips C., Hato T., Dagher P., Wu X.R., El-Achkar T.M, & Micanovic R. (2019). Circulating Uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel. Science translational medicine, 11(512), eaaw3639.

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Quantitative Proteomics of KCNQ1/KCNE1 Channels

Cited 2 times

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HEK293 cells expressing hKCNQ1 / hKCNE1 were incubated with ISO 100 nM. Cells were lysed and enriched membrane preparations (100 μg) were reduced, alkylated and digested with trypsin.^{16 (link)} Digested samples were analyzed using a Thermo-Finnigan linear ion-trap (LTQ) mass spectrometer coupled with a Surveyor autosampler and MS HPLC system (ThermoFinnigan^®). The complete LCMS/MS methods have been previously described in detail.^{16 (link)} Only proteins and peptides with protein probability ≥ 0.9000 and peptide probability ≥ 0.8000 are reported. Protein quantification was performed using a label-free quantification software package, IdentiQuantXL^™.^{17 (link)}

Shugg T., Johnson D.E., Shao M., Lai X., Witzmann F., Cummins T.R., Rubart-Von-der Lohe M., Hudmon A, & Overholser B.R. (2018). Calcium/Calmodulin-Dependent Protein Kinase II Regulation of IKs during Sustained Beta-Adrenergic Receptor Stimulation. Heart rhythm, 15(6), 895-904.

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