Vs120 automated slide scanning microscope

All animal procedures were approved by Boston University IACUC (PROTO201800533). The mouse tail-vein injection model was used to study the development of metastatic cancer. 6-week-old male homozygous nude (Foxn1nu/Foxn1nu) mice obtained from the Jackson Laboratory were used. 1205Lu melanoma cells stabling expressing GFP (Applied Biological Materials) were transfected with lentivirus carrying negative control shRNA (1205Lu-shNC) or FADS2 targeting shRNA (1205Lu-shFADS2 #2). The cells were then prepared in sterile PBS in a completely monocellular suspension without clumps at $1\times {10}^6\text{m}{\text{L}}^{-1}$ concentration. 100 μL of cell suspension was injected slowly via the lateral tail vein of the anesthetized mouse, after which the bleeding was stopped by applying pressure to the puncture site with a dry piece of gauze. The lung tissues were collected 46 days after tumor inoculation and sliced using a cryostat at 10 to 20 μm thickness. Then, the tissues were mounted with the antifade mounting medium with DAPI (Vector Laboratories). The tissues were imaged using an Olympus VS120 Automated Slide Scanning Microscope.